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Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds

Mycoplasma bovis in cattle is difficult to diagnose. Recently, the ID screen(®) mycoplasma bovis indirect ELISA (ID screen) was commercially released by IDVet. The objectives of this study were to: (1) gain and share experience of using the ID screen in adult dairy cows under field conditions; (2) d...

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Autores principales: Petersen, Mette Bisgaard, Pedersen, Lars, Pedersen, Lone Møller, Nielsen, Liza Rosenbaum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460496/
https://www.ncbi.nlm.nih.gov/pubmed/32781506
http://dx.doi.org/10.3390/pathogens9080637
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author Petersen, Mette Bisgaard
Pedersen, Lars
Pedersen, Lone Møller
Nielsen, Liza Rosenbaum
author_facet Petersen, Mette Bisgaard
Pedersen, Lars
Pedersen, Lone Møller
Nielsen, Liza Rosenbaum
author_sort Petersen, Mette Bisgaard
collection PubMed
description Mycoplasma bovis in cattle is difficult to diagnose. Recently, the ID screen(®) mycoplasma bovis indirect ELISA (ID screen) was commercially released by IDVet. The objectives of this study were to: (1) gain and share experience of using the ID screen in adult dairy cows under field conditions; (2) determine the correlation between antibody levels in milk and serum and (3) compare the ID screen results with those of the Bio K 302 (BioX 302) ELISA from BioX Diagnostics. Paired serum and milk samples were collected from 270 cows from 12 Danish dairy herds with three categories of M. bovis disease history. The ID screen tested nearly all cows positive in all, but the three non-infected herds, while the BioX 302 tested very few cows positive. The ID screen is therefore a much more sensitive test than the BioX 302. However, cows in five exposed herds without signs of ongoing infection and two herds with no history of M. bovis infection also tested ID screen positive. Therefore, the performance and interpretation of the test must be investigated under field conditions in best practice test evaluation setups. A concordance correlation coefficient of 0.66 (95% CI: 0.59–0.72) between the ID screen serum and milk results indicates that milk samples can replace serum samples for the ID screen diagnosis of M. bovis in adult cows.
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spelling pubmed-74604962020-09-03 Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds Petersen, Mette Bisgaard Pedersen, Lars Pedersen, Lone Møller Nielsen, Liza Rosenbaum Pathogens Article Mycoplasma bovis in cattle is difficult to diagnose. Recently, the ID screen(®) mycoplasma bovis indirect ELISA (ID screen) was commercially released by IDVet. The objectives of this study were to: (1) gain and share experience of using the ID screen in adult dairy cows under field conditions; (2) determine the correlation between antibody levels in milk and serum and (3) compare the ID screen results with those of the Bio K 302 (BioX 302) ELISA from BioX Diagnostics. Paired serum and milk samples were collected from 270 cows from 12 Danish dairy herds with three categories of M. bovis disease history. The ID screen tested nearly all cows positive in all, but the three non-infected herds, while the BioX 302 tested very few cows positive. The ID screen is therefore a much more sensitive test than the BioX 302. However, cows in five exposed herds without signs of ongoing infection and two herds with no history of M. bovis infection also tested ID screen positive. Therefore, the performance and interpretation of the test must be investigated under field conditions in best practice test evaluation setups. A concordance correlation coefficient of 0.66 (95% CI: 0.59–0.72) between the ID screen serum and milk results indicates that milk samples can replace serum samples for the ID screen diagnosis of M. bovis in adult cows. MDPI 2020-08-06 /pmc/articles/PMC7460496/ /pubmed/32781506 http://dx.doi.org/10.3390/pathogens9080637 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petersen, Mette Bisgaard
Pedersen, Lars
Pedersen, Lone Møller
Nielsen, Liza Rosenbaum
Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title_full Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title_fullStr Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title_full_unstemmed Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title_short Field Experience of Antibody Testing against Mycoplasma bovis in Adult Cows in Commercial Danish Dairy Cattle Herds
title_sort field experience of antibody testing against mycoplasma bovis in adult cows in commercial danish dairy cattle herds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460496/
https://www.ncbi.nlm.nih.gov/pubmed/32781506
http://dx.doi.org/10.3390/pathogens9080637
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