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Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alg...

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Autores principales: Ma, Yan, Li, Jie, Zhang, Xin-Yue, Ni, Hao-Dong, Wang, Feng-Biao, Wang, Hai-Ying, Wang, Zhi-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460510/
https://www.ncbi.nlm.nih.gov/pubmed/32784864
http://dx.doi.org/10.3390/md18080416
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author Ma, Yan
Li, Jie
Zhang, Xin-Yue
Ni, Hao-Dong
Wang, Feng-Biao
Wang, Hai-Ying
Wang, Zhi-Peng
author_facet Ma, Yan
Li, Jie
Zhang, Xin-Yue
Ni, Hao-Dong
Wang, Feng-Biao
Wang, Hai-Ying
Wang, Zhi-Peng
author_sort Ma, Yan
collection PubMed
description Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.
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spelling pubmed-74605102020-09-03 Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties Ma, Yan Li, Jie Zhang, Xin-Yue Ni, Hao-Dong Wang, Feng-Biao Wang, Hai-Ying Wang, Zhi-Peng Mar Drugs Article Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries. MDPI 2020-08-09 /pmc/articles/PMC7460510/ /pubmed/32784864 http://dx.doi.org/10.3390/md18080416 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Yan
Li, Jie
Zhang, Xin-Yue
Ni, Hao-Dong
Wang, Feng-Biao
Wang, Hai-Ying
Wang, Zhi-Peng
Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title_full Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title_fullStr Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title_full_unstemmed Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title_short Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
title_sort characterization of a new intracellular alginate lyase with metal ions-tolerant and ph-stable properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460510/
https://www.ncbi.nlm.nih.gov/pubmed/32784864
http://dx.doi.org/10.3390/md18080416
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