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Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alg...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460510/ https://www.ncbi.nlm.nih.gov/pubmed/32784864 http://dx.doi.org/10.3390/md18080416 |
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author | Ma, Yan Li, Jie Zhang, Xin-Yue Ni, Hao-Dong Wang, Feng-Biao Wang, Hai-Ying Wang, Zhi-Peng |
author_facet | Ma, Yan Li, Jie Zhang, Xin-Yue Ni, Hao-Dong Wang, Feng-Biao Wang, Hai-Ying Wang, Zhi-Peng |
author_sort | Ma, Yan |
collection | PubMed |
description | Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries. |
format | Online Article Text |
id | pubmed-7460510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74605102020-09-03 Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties Ma, Yan Li, Jie Zhang, Xin-Yue Ni, Hao-Dong Wang, Feng-Biao Wang, Hai-Ying Wang, Zhi-Peng Mar Drugs Article Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries. MDPI 2020-08-09 /pmc/articles/PMC7460510/ /pubmed/32784864 http://dx.doi.org/10.3390/md18080416 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Yan Li, Jie Zhang, Xin-Yue Ni, Hao-Dong Wang, Feng-Biao Wang, Hai-Ying Wang, Zhi-Peng Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title | Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title_full | Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title_fullStr | Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title_full_unstemmed | Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title_short | Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties |
title_sort | characterization of a new intracellular alginate lyase with metal ions-tolerant and ph-stable properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460510/ https://www.ncbi.nlm.nih.gov/pubmed/32784864 http://dx.doi.org/10.3390/md18080416 |
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