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Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460526/ https://www.ncbi.nlm.nih.gov/pubmed/32823904 http://dx.doi.org/10.3390/antibiotics9080517 |
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author | Sangboonruang, Sirikwan Kitidee, Kuntida Chantawannakul, Panuwan Tragoolpua, Khajornsak Tragoolpua, Yingmanee |
author_facet | Sangboonruang, Sirikwan Kitidee, Kuntida Chantawannakul, Panuwan Tragoolpua, Khajornsak Tragoolpua, Yingmanee |
author_sort | Sangboonruang, Sirikwan |
collection | PubMed |
description | Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent. |
format | Online Article Text |
id | pubmed-7460526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74605262020-09-03 Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment Sangboonruang, Sirikwan Kitidee, Kuntida Chantawannakul, Panuwan Tragoolpua, Khajornsak Tragoolpua, Yingmanee Antibiotics (Basel) Article Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent. MDPI 2020-08-14 /pmc/articles/PMC7460526/ /pubmed/32823904 http://dx.doi.org/10.3390/antibiotics9080517 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sangboonruang, Sirikwan Kitidee, Kuntida Chantawannakul, Panuwan Tragoolpua, Khajornsak Tragoolpua, Yingmanee Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title | Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title_full | Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title_fullStr | Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title_full_unstemmed | Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title_short | Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment |
title_sort | melittin from apis florea venom as a promising therapeutic agent for skin cancer treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460526/ https://www.ncbi.nlm.nih.gov/pubmed/32823904 http://dx.doi.org/10.3390/antibiotics9080517 |
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