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Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment

Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells....

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Autores principales: Sangboonruang, Sirikwan, Kitidee, Kuntida, Chantawannakul, Panuwan, Tragoolpua, Khajornsak, Tragoolpua, Yingmanee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460526/
https://www.ncbi.nlm.nih.gov/pubmed/32823904
http://dx.doi.org/10.3390/antibiotics9080517
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author Sangboonruang, Sirikwan
Kitidee, Kuntida
Chantawannakul, Panuwan
Tragoolpua, Khajornsak
Tragoolpua, Yingmanee
author_facet Sangboonruang, Sirikwan
Kitidee, Kuntida
Chantawannakul, Panuwan
Tragoolpua, Khajornsak
Tragoolpua, Yingmanee
author_sort Sangboonruang, Sirikwan
collection PubMed
description Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent.
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spelling pubmed-74605262020-09-03 Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment Sangboonruang, Sirikwan Kitidee, Kuntida Chantawannakul, Panuwan Tragoolpua, Khajornsak Tragoolpua, Yingmanee Antibiotics (Basel) Article Melittin, a major component found in bee venom, is produced by the Apis species of the honey bee. In this study, the effect of melittin derived from Apis florea (Mel-AF), which is a wild honey bee species that is indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. In this study, Mel-AF exhibited considerable potential in the anti-proliferative action of A375 cells. Subsequently, the cellular mechanism of Mel-AF that induced cell death was investigated in terms of apoptosis. As a result, gene and protein expression levels, which indicated the activation of cytochrome-c release and caspase-9 expression, eventually triggered the release of the caspase-3 executioner upon Mel-AF. We then determined that apoptosis-mediated cell death was carried out through the intrinsic mitochondrial pathway. Moreover, advanced abilities, including cell motility and invasion, were significantly suppressed. Mel-AF manipulated the actin arrangement via the trapping of stress fibers that were found underneath the membrane, which resulted in the defective actin cytoskeleton organization. Consequently, the expression of EGFR, a binding protein to F-actin, was also found to be suppressed. This outcome strongly supports the effects of Mel-AF in the inhibition of progressive malignant activity through the disruption of actin cytoskeleton-EGFR interaction and the EGFR signaling system. Thus, the findings of our current study indicate the potential usefulness of Mel-AF in cancer treatments as an apoptosis inducer and a potential actin-targeting agent. MDPI 2020-08-14 /pmc/articles/PMC7460526/ /pubmed/32823904 http://dx.doi.org/10.3390/antibiotics9080517 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sangboonruang, Sirikwan
Kitidee, Kuntida
Chantawannakul, Panuwan
Tragoolpua, Khajornsak
Tragoolpua, Yingmanee
Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title_full Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title_fullStr Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title_full_unstemmed Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title_short Melittin from Apis florea Venom as a Promising Therapeutic Agent for Skin Cancer Treatment
title_sort melittin from apis florea venom as a promising therapeutic agent for skin cancer treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460526/
https://www.ncbi.nlm.nih.gov/pubmed/32823904
http://dx.doi.org/10.3390/antibiotics9080517
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