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Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans

The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low...

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Autores principales: Pimentel-Acosta, Citlalic A., Ramírez-Salcedo, Jorge, Morales-Serna, Francisco Neptalí, Fajer-Ávila, Emma J., Chávez-Sánchez, Cristina, Lara, Humberto H., García-Gasca, Alejandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460582/
https://www.ncbi.nlm.nih.gov/pubmed/32824343
http://dx.doi.org/10.3390/ijms21165889
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author Pimentel-Acosta, Citlalic A.
Ramírez-Salcedo, Jorge
Morales-Serna, Francisco Neptalí
Fajer-Ávila, Emma J.
Chávez-Sánchez, Cristina
Lara, Humberto H.
García-Gasca, Alejandra
author_facet Pimentel-Acosta, Citlalic A.
Ramírez-Salcedo, Jorge
Morales-Serna, Francisco Neptalí
Fajer-Ávila, Emma J.
Chávez-Sánchez, Cristina
Lara, Humberto H.
García-Gasca, Alejandra
author_sort Pimentel-Acosta, Citlalic A.
collection PubMed
description The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish.
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spelling pubmed-74605822020-09-03 Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans Pimentel-Acosta, Citlalic A. Ramírez-Salcedo, Jorge Morales-Serna, Francisco Neptalí Fajer-Ávila, Emma J. Chávez-Sánchez, Cristina Lara, Humberto H. García-Gasca, Alejandra Int J Mol Sci Article The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish. MDPI 2020-08-16 /pmc/articles/PMC7460582/ /pubmed/32824343 http://dx.doi.org/10.3390/ijms21165889 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pimentel-Acosta, Citlalic A.
Ramírez-Salcedo, Jorge
Morales-Serna, Francisco Neptalí
Fajer-Ávila, Emma J.
Chávez-Sánchez, Cristina
Lara, Humberto H.
García-Gasca, Alejandra
Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title_full Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title_fullStr Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title_full_unstemmed Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title_short Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans
title_sort molecular effects of silver nanoparticles on monogenean parasites: lessons from caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460582/
https://www.ncbi.nlm.nih.gov/pubmed/32824343
http://dx.doi.org/10.3390/ijms21165889
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