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Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function

Uromodulin and microRNAs (miRNAs) have recently been investigated as potential biomarkers for kidney graft associated pathology and outcome, with a special focus on biomarkers indicating specific disease processes and kidney graft survival. The study’s aim was to determine whether expression of seru...

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Autores principales: Borštnar, Špela, Večerić-Haler, Željka, Boštjančič, Emanuela, Pipan Tkalec, Živa, Kovač, Damjan, Lindič, Jelka, Kojc, Nika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460670/
https://www.ncbi.nlm.nih.gov/pubmed/32764335
http://dx.doi.org/10.3390/ijms21165592
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author Borštnar, Špela
Večerić-Haler, Željka
Boštjančič, Emanuela
Pipan Tkalec, Živa
Kovač, Damjan
Lindič, Jelka
Kojc, Nika
author_facet Borštnar, Špela
Večerić-Haler, Željka
Boštjančič, Emanuela
Pipan Tkalec, Živa
Kovač, Damjan
Lindič, Jelka
Kojc, Nika
author_sort Borštnar, Špela
collection PubMed
description Uromodulin and microRNAs (miRNAs) have recently been investigated as potential biomarkers for kidney graft associated pathology and outcome, with a special focus on biomarkers indicating specific disease processes and kidney graft survival. The study’s aim was to determine whether expression of serum uromodulin concentration and selected miRNAs might be related to renal function in kidney transplant recipients (KTRs). The uromodulin concentration and expression of six selected miRNAs (miR-29c, miR-126, miR-146a, miR-150, miR-155, and miR-223) were determined in the serum of 100 KTRs with stable graft function and chronic kidney disease of all five stages. Kidney graft function was estimated with routine parameters (creatinine, urea, cystatin C, and Chronic Kidney Disease Epidemiology Collaboration study equations) and precisely measured using chromium-51 labelled ethylenediaminetetraacetic-acid clearance. The selected miRNAs were shown to be independent of kidney graft function, indicating their potential as biomarkers of associated kidney graft disease processes. In contrast, the serum uromodulin level depended entirely on kidney graft function and thus reflected functioning tubules rather than any specific kidney graft injury. However, decreased concentrations of serum uromodulin can be observed in the early course of tubulointerstitial injury, thereby suggesting its useful role as an accurate, noninvasive biomarker of early (subclinical) kidney graft injury.
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spelling pubmed-74606702020-09-03 Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function Borštnar, Špela Večerić-Haler, Željka Boštjančič, Emanuela Pipan Tkalec, Živa Kovač, Damjan Lindič, Jelka Kojc, Nika Int J Mol Sci Article Uromodulin and microRNAs (miRNAs) have recently been investigated as potential biomarkers for kidney graft associated pathology and outcome, with a special focus on biomarkers indicating specific disease processes and kidney graft survival. The study’s aim was to determine whether expression of serum uromodulin concentration and selected miRNAs might be related to renal function in kidney transplant recipients (KTRs). The uromodulin concentration and expression of six selected miRNAs (miR-29c, miR-126, miR-146a, miR-150, miR-155, and miR-223) were determined in the serum of 100 KTRs with stable graft function and chronic kidney disease of all five stages. Kidney graft function was estimated with routine parameters (creatinine, urea, cystatin C, and Chronic Kidney Disease Epidemiology Collaboration study equations) and precisely measured using chromium-51 labelled ethylenediaminetetraacetic-acid clearance. The selected miRNAs were shown to be independent of kidney graft function, indicating their potential as biomarkers of associated kidney graft disease processes. In contrast, the serum uromodulin level depended entirely on kidney graft function and thus reflected functioning tubules rather than any specific kidney graft injury. However, decreased concentrations of serum uromodulin can be observed in the early course of tubulointerstitial injury, thereby suggesting its useful role as an accurate, noninvasive biomarker of early (subclinical) kidney graft injury. MDPI 2020-08-05 /pmc/articles/PMC7460670/ /pubmed/32764335 http://dx.doi.org/10.3390/ijms21165592 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borštnar, Špela
Večerić-Haler, Željka
Boštjančič, Emanuela
Pipan Tkalec, Živa
Kovač, Damjan
Lindič, Jelka
Kojc, Nika
Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title_full Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title_fullStr Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title_full_unstemmed Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title_short Uromodulin and microRNAs in Kidney Transplantation—Association with Kidney Graft Function
title_sort uromodulin and micrornas in kidney transplantation—association with kidney graft function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460670/
https://www.ncbi.nlm.nih.gov/pubmed/32764335
http://dx.doi.org/10.3390/ijms21165592
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