Cargando…

In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?

In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell...

Descripción completa

Detalles Bibliográficos
Autores principales: Clara-Trujillo, Sandra, Gallego Ferrer, Gloria, Gómez Ribelles, José Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460836/
https://www.ncbi.nlm.nih.gov/pubmed/32796596
http://dx.doi.org/10.3390/ijms21165747
_version_ 1783576687793930240
author Clara-Trujillo, Sandra
Gallego Ferrer, Gloria
Gómez Ribelles, José Luis
author_facet Clara-Trujillo, Sandra
Gallego Ferrer, Gloria
Gómez Ribelles, José Luis
author_sort Clara-Trujillo, Sandra
collection PubMed
description In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell fate under physiological and pathological conditions and serve as a reference for the native biological landscape to be replicated in engineered platforms attempting to reproduce blood cancer behavior. This review summarizes the different models used to efficiently reproduce certain aspects of BM in vitro; however, they still lack the complexity of this tissue, which is relevant for fundamental aspects such as drug resistance development in multiple myeloma. Extracellular matrix composition, material topography, vascularization, cellular composition or stemness vs. differentiation balance are discussed as variables that could be rationally defined in tissue engineering approaches for achieving more relevant in vitro models. Fully humanized platforms closely resembling natural interactions still remain challenging and the question of to what extent accurate tissue complexity reproduction is essential to reliably predict drug responses is controversial. However, the contributions of these approaches to the fundamental knowledge of non-solid tumor biology, its regulation by niches, and the advance of personalized medicine are unquestionable.
format Online
Article
Text
id pubmed-7460836
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74608362020-09-03 In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? Clara-Trujillo, Sandra Gallego Ferrer, Gloria Gómez Ribelles, José Luis Int J Mol Sci Review In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell fate under physiological and pathological conditions and serve as a reference for the native biological landscape to be replicated in engineered platforms attempting to reproduce blood cancer behavior. This review summarizes the different models used to efficiently reproduce certain aspects of BM in vitro; however, they still lack the complexity of this tissue, which is relevant for fundamental aspects such as drug resistance development in multiple myeloma. Extracellular matrix composition, material topography, vascularization, cellular composition or stemness vs. differentiation balance are discussed as variables that could be rationally defined in tissue engineering approaches for achieving more relevant in vitro models. Fully humanized platforms closely resembling natural interactions still remain challenging and the question of to what extent accurate tissue complexity reproduction is essential to reliably predict drug responses is controversial. However, the contributions of these approaches to the fundamental knowledge of non-solid tumor biology, its regulation by niches, and the advance of personalized medicine are unquestionable. MDPI 2020-08-11 /pmc/articles/PMC7460836/ /pubmed/32796596 http://dx.doi.org/10.3390/ijms21165747 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Clara-Trujillo, Sandra
Gallego Ferrer, Gloria
Gómez Ribelles, José Luis
In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title_full In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title_fullStr In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title_full_unstemmed In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title_short In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
title_sort in vitro modeling of non-solid tumors: how far can tissue engineering go?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460836/
https://www.ncbi.nlm.nih.gov/pubmed/32796596
http://dx.doi.org/10.3390/ijms21165747
work_keys_str_mv AT claratrujillosandra invitromodelingofnonsolidtumorshowfarcantissueengineeringgo
AT gallegoferrergloria invitromodelingofnonsolidtumorshowfarcantissueengineeringgo
AT gomezribellesjoseluis invitromodelingofnonsolidtumorshowfarcantissueengineeringgo