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In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460836/ https://www.ncbi.nlm.nih.gov/pubmed/32796596 http://dx.doi.org/10.3390/ijms21165747 |
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author | Clara-Trujillo, Sandra Gallego Ferrer, Gloria Gómez Ribelles, José Luis |
author_facet | Clara-Trujillo, Sandra Gallego Ferrer, Gloria Gómez Ribelles, José Luis |
author_sort | Clara-Trujillo, Sandra |
collection | PubMed |
description | In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell fate under physiological and pathological conditions and serve as a reference for the native biological landscape to be replicated in engineered platforms attempting to reproduce blood cancer behavior. This review summarizes the different models used to efficiently reproduce certain aspects of BM in vitro; however, they still lack the complexity of this tissue, which is relevant for fundamental aspects such as drug resistance development in multiple myeloma. Extracellular matrix composition, material topography, vascularization, cellular composition or stemness vs. differentiation balance are discussed as variables that could be rationally defined in tissue engineering approaches for achieving more relevant in vitro models. Fully humanized platforms closely resembling natural interactions still remain challenging and the question of to what extent accurate tissue complexity reproduction is essential to reliably predict drug responses is controversial. However, the contributions of these approaches to the fundamental knowledge of non-solid tumor biology, its regulation by niches, and the advance of personalized medicine are unquestionable. |
format | Online Article Text |
id | pubmed-7460836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74608362020-09-03 In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? Clara-Trujillo, Sandra Gallego Ferrer, Gloria Gómez Ribelles, José Luis Int J Mol Sci Review In hematological malignancies, leukemias or myelomas, malignant cells present bone marrow (BM) homing, in which the niche contributes to tumor development and drug resistance. BM architecture, cellular and molecular composition and interactions define differential microenvironments that govern cell fate under physiological and pathological conditions and serve as a reference for the native biological landscape to be replicated in engineered platforms attempting to reproduce blood cancer behavior. This review summarizes the different models used to efficiently reproduce certain aspects of BM in vitro; however, they still lack the complexity of this tissue, which is relevant for fundamental aspects such as drug resistance development in multiple myeloma. Extracellular matrix composition, material topography, vascularization, cellular composition or stemness vs. differentiation balance are discussed as variables that could be rationally defined in tissue engineering approaches for achieving more relevant in vitro models. Fully humanized platforms closely resembling natural interactions still remain challenging and the question of to what extent accurate tissue complexity reproduction is essential to reliably predict drug responses is controversial. However, the contributions of these approaches to the fundamental knowledge of non-solid tumor biology, its regulation by niches, and the advance of personalized medicine are unquestionable. MDPI 2020-08-11 /pmc/articles/PMC7460836/ /pubmed/32796596 http://dx.doi.org/10.3390/ijms21165747 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Clara-Trujillo, Sandra Gallego Ferrer, Gloria Gómez Ribelles, José Luis In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title | In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title_full | In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title_fullStr | In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title_full_unstemmed | In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title_short | In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go? |
title_sort | in vitro modeling of non-solid tumors: how far can tissue engineering go? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460836/ https://www.ncbi.nlm.nih.gov/pubmed/32796596 http://dx.doi.org/10.3390/ijms21165747 |
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