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Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB in the regulat...

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Autores principales: Germanà, Antonino, Guerrera, Maria Cristina, Laurà, Rosaria, Levanti, Maria, Aragona, Marialuisa, Mhalhel, Kamel, Germanà, Germana, Montalbano, Giuseppe, Abbate, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460859/
https://www.ncbi.nlm.nih.gov/pubmed/32806650
http://dx.doi.org/10.3390/ijms21165787
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author Germanà, Antonino
Guerrera, Maria Cristina
Laurà, Rosaria
Levanti, Maria
Aragona, Marialuisa
Mhalhel, Kamel
Germanà, Germana
Montalbano, Giuseppe
Abbate, Francesco
author_facet Germanà, Antonino
Guerrera, Maria Cristina
Laurà, Rosaria
Levanti, Maria
Aragona, Marialuisa
Mhalhel, Kamel
Germanà, Germana
Montalbano, Giuseppe
Abbate, Francesco
author_sort Germanà, Antonino
collection PubMed
description Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB in the regulation of the acoustic system of mammals, the localization of BDNF/TrkB system in the inner ear of zebrafish during development, is not well known. Therefore, the goal of the present study is to analyze the age-dependent changes using RT-PCR, Western Blot and single and double immunofluorescence of the BDNF and its specific receptor in the zebrafish inner ear. The results showed the mRNA expression and the cell localization of BDNF and TrkB in the hair cells of the crista ampullaris and in the neuroepithelium of the utricle, saccule and macula lagena, analyzed at different ages. Our results demonstrate that the BDNF/TrkB system is present in the sensory cells of the inner ear, during whole life. Therefore, this system might play a key role in the development and maintenance of the hair cells in adults, suggesting that the zebrafish inner ear represents an interesting model to study the involvement of the neurotrophins in the biology of sensory cells
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spelling pubmed-74608592020-09-14 Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear Germanà, Antonino Guerrera, Maria Cristina Laurà, Rosaria Levanti, Maria Aragona, Marialuisa Mhalhel, Kamel Germanà, Germana Montalbano, Giuseppe Abbate, Francesco Int J Mol Sci Article Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB in the regulation of the acoustic system of mammals, the localization of BDNF/TrkB system in the inner ear of zebrafish during development, is not well known. Therefore, the goal of the present study is to analyze the age-dependent changes using RT-PCR, Western Blot and single and double immunofluorescence of the BDNF and its specific receptor in the zebrafish inner ear. The results showed the mRNA expression and the cell localization of BDNF and TrkB in the hair cells of the crista ampullaris and in the neuroepithelium of the utricle, saccule and macula lagena, analyzed at different ages. Our results demonstrate that the BDNF/TrkB system is present in the sensory cells of the inner ear, during whole life. Therefore, this system might play a key role in the development and maintenance of the hair cells in adults, suggesting that the zebrafish inner ear represents an interesting model to study the involvement of the neurotrophins in the biology of sensory cells MDPI 2020-08-12 /pmc/articles/PMC7460859/ /pubmed/32806650 http://dx.doi.org/10.3390/ijms21165787 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Germanà, Antonino
Guerrera, Maria Cristina
Laurà, Rosaria
Levanti, Maria
Aragona, Marialuisa
Mhalhel, Kamel
Germanà, Germana
Montalbano, Giuseppe
Abbate, Francesco
Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title_full Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title_fullStr Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title_full_unstemmed Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title_short Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear
title_sort expression and localization of bdnf/trkb system in the zebrafish inner ear
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460859/
https://www.ncbi.nlm.nih.gov/pubmed/32806650
http://dx.doi.org/10.3390/ijms21165787
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