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Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection

Sensitive molecular assays are critical for coronavirus disease 2019 (COVID-19) diagnosis. Here, we designed and evaluated two single-tube nested (STN) real-time RT-PCR assays, targeting SARS-CoV-2 RdRp/Hel and N genes. Both STN assays had a low limit of detection and did not cross react with other...

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Detalles Bibliográficos
Autores principales:	Yip, Cyril Chik-Yan, Sridhar, Siddharth, Leung, Kit-Hang, Ng, Anthony Chin-Ki, Chan, Kwok-Hung, Chan, Jasper Fuk-Woo, Tsang, Owen Tak-Yin, Hung, Ivan Fan-Ngai, Cheng, Vincent Chi-Chung, Yuen, Kwok-Yung, To, Kelvin Kai-Wang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461039/ https://www.ncbi.nlm.nih.gov/pubmed/32784770 http://dx.doi.org/10.3390/ijms21165674

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