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Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion
Non-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461124/ https://www.ncbi.nlm.nih.gov/pubmed/32785145 http://dx.doi.org/10.3390/ijms21165739 |
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author | Wegele, Christian Stump-Guthier, Carolin Moroniak, Selina Weiss, Christel Rohde, Manfred Ishikawa, Hiroshi Schroten, Horst Schwerk, Christian Karremann, Michael Borkowski, Julia |
author_facet | Wegele, Christian Stump-Guthier, Carolin Moroniak, Selina Weiss, Christel Rohde, Manfred Ishikawa, Hiroshi Schroten, Horst Schwerk, Christian Karremann, Michael Borkowski, Julia |
author_sort | Wegele, Christian |
collection | PubMed |
description | Non-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance of NTHI impede treatment and emphasize its relevance as a potential meningitis causing pathogen. Meningitis results from pathogens crossing blood–brain barriers and invading the immune privileged central nervous system (CNS). In this study, we addressed the potential of NTHI to enter the brain by invading cells of the choroid plexus (CP) prior to meningeal inflammation to enlighten NTHI pathophysiological mechanisms. A cell culture model of human CP epithelial cells, which form the blood–cerebrospinal fluid barrier (BCSFB) in vivo, was used to analyze adhesion and invasion by immunofluorescence and electron microscopy. NTHI invade CP cells in vitro in a polar fashion from the blood-facing side. Furthermore, NTHI invasion rates are increased compared to encapsulated HiB and HiF strains. Fimbriae occurrence attenuated adhesion and invasion. Thus, our findings underline the role of the BCSFB as a potential entry port for NTHI into the brain and provide strong evidence for a function of the CP during NTHI invasion into the CNS during the course of meningitis. |
format | Online Article Text |
id | pubmed-7461124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74611242020-09-14 Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion Wegele, Christian Stump-Guthier, Carolin Moroniak, Selina Weiss, Christel Rohde, Manfred Ishikawa, Hiroshi Schroten, Horst Schwerk, Christian Karremann, Michael Borkowski, Julia Int J Mol Sci Article Non-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance of NTHI impede treatment and emphasize its relevance as a potential meningitis causing pathogen. Meningitis results from pathogens crossing blood–brain barriers and invading the immune privileged central nervous system (CNS). In this study, we addressed the potential of NTHI to enter the brain by invading cells of the choroid plexus (CP) prior to meningeal inflammation to enlighten NTHI pathophysiological mechanisms. A cell culture model of human CP epithelial cells, which form the blood–cerebrospinal fluid barrier (BCSFB) in vivo, was used to analyze adhesion and invasion by immunofluorescence and electron microscopy. NTHI invade CP cells in vitro in a polar fashion from the blood-facing side. Furthermore, NTHI invasion rates are increased compared to encapsulated HiB and HiF strains. Fimbriae occurrence attenuated adhesion and invasion. Thus, our findings underline the role of the BCSFB as a potential entry port for NTHI into the brain and provide strong evidence for a function of the CP during NTHI invasion into the CNS during the course of meningitis. MDPI 2020-08-10 /pmc/articles/PMC7461124/ /pubmed/32785145 http://dx.doi.org/10.3390/ijms21165739 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wegele, Christian Stump-Guthier, Carolin Moroniak, Selina Weiss, Christel Rohde, Manfred Ishikawa, Hiroshi Schroten, Horst Schwerk, Christian Karremann, Michael Borkowski, Julia Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title | Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title_full | Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title_fullStr | Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title_full_unstemmed | Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title_short | Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion |
title_sort | non-typeable haemophilus influenzae invade choroid plexus epithelial cells in a polar fashion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461124/ https://www.ncbi.nlm.nih.gov/pubmed/32785145 http://dx.doi.org/10.3390/ijms21165739 |
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