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Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III
The growth of Legionella dumoffii can be inhibited by Galleria mellonella apolipophorin III (apoLp-III) which is an insect homologue of human apolipoprotein E., and choline-cultured L. dumoffii cells are considerably more susceptible to apoLp-III than bacteria grown without choline supplementation....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461559/ https://www.ncbi.nlm.nih.gov/pubmed/32823647 http://dx.doi.org/10.3390/ijms21165818 |
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author | Palusińska-Szysz, Marta Zdybicka-Barabas, Agnieszka Luchowski, Rafał Reszczyńska, Emilia Śmiałek, Justyna Mak, Paweł Gruszecki, Wiesław I. Cytryńska, Małgorzata |
author_facet | Palusińska-Szysz, Marta Zdybicka-Barabas, Agnieszka Luchowski, Rafał Reszczyńska, Emilia Śmiałek, Justyna Mak, Paweł Gruszecki, Wiesław I. Cytryńska, Małgorzata |
author_sort | Palusińska-Szysz, Marta |
collection | PubMed |
description | The growth of Legionella dumoffii can be inhibited by Galleria mellonella apolipophorin III (apoLp-III) which is an insect homologue of human apolipoprotein E., and choline-cultured L. dumoffii cells are considerably more susceptible to apoLp-III than bacteria grown without choline supplementation. In the present study, the interactions of apoLp-III with intact L. dumoffii cells cultured without and with exogenous choline were analyzed to explain the basis of this difference. Fluorescently labeled apoLp-III (FITC-apoLp-III) bound more efficiently to choline-grown L. dumoffii, as revealed by laser scanning confocal microscopy. The cell envelope of these bacteria was penetrated more deeply by FITC-apoLp-III, as demonstrated by fluorescence lifetime imaging microscopy analyses. The increased susceptibility of the choline-cultured L. dumoffii to apoLp-III was also accompanied by alterations in the cell surface topography and nanomechanical properties. A detailed analysis of the interaction of apoLp-III with components of the L. dumoffii cells was carried out using both purified lipopolysaccharide (LPS) and liposomes composed of L. dumoffii phospholipids and LPS. A single micelle of L. dumoffii LPS was formed from 12 to 29 monomeric LPS molecules and one L. dumoffii LPS micelle bound two molecules of apoLp-III. ApoLp-III exhibited the strongest interactions with liposomes with incorporated LPS formed of phospholipids isolated from bacteria cultured on exogenous choline. These results indicated that the differences in the phospholipid content in the cell membrane, especially PC, and LPS affected the interactions of apoLp-III with bacterial cells and suggested that these differences contributed to the increased susceptibility of the choline-cultured L. dumoffii to G. mellonella apoLp-III. |
format | Online Article Text |
id | pubmed-7461559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74615592020-09-04 Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III Palusińska-Szysz, Marta Zdybicka-Barabas, Agnieszka Luchowski, Rafał Reszczyńska, Emilia Śmiałek, Justyna Mak, Paweł Gruszecki, Wiesław I. Cytryńska, Małgorzata Int J Mol Sci Article The growth of Legionella dumoffii can be inhibited by Galleria mellonella apolipophorin III (apoLp-III) which is an insect homologue of human apolipoprotein E., and choline-cultured L. dumoffii cells are considerably more susceptible to apoLp-III than bacteria grown without choline supplementation. In the present study, the interactions of apoLp-III with intact L. dumoffii cells cultured without and with exogenous choline were analyzed to explain the basis of this difference. Fluorescently labeled apoLp-III (FITC-apoLp-III) bound more efficiently to choline-grown L. dumoffii, as revealed by laser scanning confocal microscopy. The cell envelope of these bacteria was penetrated more deeply by FITC-apoLp-III, as demonstrated by fluorescence lifetime imaging microscopy analyses. The increased susceptibility of the choline-cultured L. dumoffii to apoLp-III was also accompanied by alterations in the cell surface topography and nanomechanical properties. A detailed analysis of the interaction of apoLp-III with components of the L. dumoffii cells was carried out using both purified lipopolysaccharide (LPS) and liposomes composed of L. dumoffii phospholipids and LPS. A single micelle of L. dumoffii LPS was formed from 12 to 29 monomeric LPS molecules and one L. dumoffii LPS micelle bound two molecules of apoLp-III. ApoLp-III exhibited the strongest interactions with liposomes with incorporated LPS formed of phospholipids isolated from bacteria cultured on exogenous choline. These results indicated that the differences in the phospholipid content in the cell membrane, especially PC, and LPS affected the interactions of apoLp-III with bacterial cells and suggested that these differences contributed to the increased susceptibility of the choline-cultured L. dumoffii to G. mellonella apoLp-III. MDPI 2020-08-13 /pmc/articles/PMC7461559/ /pubmed/32823647 http://dx.doi.org/10.3390/ijms21165818 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Palusińska-Szysz, Marta Zdybicka-Barabas, Agnieszka Luchowski, Rafał Reszczyńska, Emilia Śmiałek, Justyna Mak, Paweł Gruszecki, Wiesław I. Cytryńska, Małgorzata Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title | Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title_full | Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title_fullStr | Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title_full_unstemmed | Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title_short | Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III |
title_sort | choline supplementation sensitizes legionella dumoffii to galleria mellonella apolipophorin iii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461559/ https://www.ncbi.nlm.nih.gov/pubmed/32823647 http://dx.doi.org/10.3390/ijms21165818 |
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