Cargando…

Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases

Modification dependent restriction endonucleases (MDREs) restrict modified DNA, typically with limited sequence specificity (∼2–4 bp). Here, we focus on MDREs that have an SRA and/or SBD (sulfur binding domain) fused to an HNH endonuclease domain, cleaving cytosine modified or phosphorothioated (PT)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lutz, Thomas, Czapinska, Honorata, Fomenkov, Alexey, Potapov, Vladimir, Heiter, Daniel F., Cao, Bo, Dedon, Peter, Bochtler, Matthias, Xu, Shuang-yong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461809/ https://www.ncbi.nlm.nih.gov/pubmed/33013736 http://dx.doi.org/10.3389/fmicb.2020.01960

Ejemplares similares

A protein architecture guided screen for modification dependent restriction endonucleases
por: Lutz, Thomas, et al.
Publicado: (2019)

On the role of steric clashes in methylation control of restriction endonuclease activity
por: Mierzejewska, Karolina, et al.
Publicado: (2016)

Crystal structure of the ββα-Me type II restriction endonuclease Hpy99I with target DNA
por: Sokolowska, Monika, et al.
Publicado: (2009)

High-throughput sequencing of EcoWI restriction fragments maps the genome-wide landscape of phosphorothioate modification at base resolution
por: Yang, Weiwei, et al.
Publicado: (2022)

Crystal structure of the modification-dependent SRA-HNH endonuclease TagI
por: Kisiala, Marlena, et al.
Publicado: (2018)

Crystal structure and mechanism of action of the N6-methyladenine-dependent type IIM restriction endonuclease R.DpnI
por: Siwek, Wojciech, et al.
Publicado: (2012)

Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation
por: Kisiala, Marlena, et al.
Publicado: (2020)

Crystal structures of the EVE-HNH endonuclease VcaM4I in the presence and absence of DNA
por: Pastor, Michal, et al.
Publicado: (2021)

Crystal structure of the 5hmC specific endonuclease PvuRts1I
por: Kazrani, Asgar Abbas, et al.
Publicado: (2014)

Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically
por: Kaus-Drobek, Magdalena, et al.
Publicado: (2007)

Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity From BsaXI Specificity Subunit
por: Gidwani, Sonal, et al.
Publicado: (2022)

The Nϵ‐Rule for Serine, but Not Cysteine Catalytic Triads
por: Czapinska, Honorata, et al.
Publicado: (2022)

Crystal structure of the EcoKMcrA N-terminal domain (NEco): recognition of modified cytosine bases without flipping
por: Slyvka, Anton, et al.
Publicado: (2019)

Hpy188I–DNA pre- and post-cleavage complexes—snapshots of the GIY-YIG nuclease mediated catalysis
por: Sokolowska, Monika, et al.
Publicado: (2011)

DNA intercalation without flipping in the specific ThaI–DNA complex
por: Firczuk, Malgorzata, et al.
Publicado: (2011)

Nanopore Sequencing for Detection and Characterization of Phosphorothioate Modifications in Native DNA Sequences
por: Wadley, Taylor, et al.
Publicado: (2022)

Cleavage of Phosphorothioated DNA and Methylated DNA by the Type IV Restriction Endonuclease ScoMcrA
por: Liu, Guang, et al.
Publicado: (2010)

High resolution structure of an M23 peptidase with a substrate analogue
por: Grabowska, Maja, et al.
Publicado: (2015)

Enzymatic Cleavage of Type II Restriction Endonucleases on the 2′-O-Methyl Nucleotide and Phosphorothioate Substituted DNA
por: Zhao, Guojie, et al.
Publicado: (2013)

Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI
por: Horton, John R., et al.
Publicado: (2014)

Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family
por: Tamulaitiene, Giedre, et al.
Publicado: (2019)

Halogen Atoms in the Protein–Ligand System. Structural and Thermodynamic Studies of the Binding of Bromobenzotriazoles by the Catalytic Subunit of Human Protein Kinase CK2
por: Czapinska, Honorata, et al.
Publicado: (2021)

Activity and structure of EcoKMcrA
por: Czapinska, Honorata, et al.
Publicado: (2018)

Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase A
por: Xu, Shuang-yong, et al.
Publicado: (2021)

Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
por: Chan, Siu-hong, et al.
Publicado: (2007)

Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI
por: Shen, Betty W, et al.
Publicado: (2019)

Interactions of Dnd proteins involved in bacterial DNA phosphorothioate modification
por: Xiong, Wei, et al.
Publicado: (2015)

The road less traveled: A new phosphorothioate antiviral defense mechanism discovered in Archaea
por: DeMott, Michael S., et al.
Publicado: (2019)

DNA Phosphorothioate Modifications Are Widely Distributed in the Human Microbiome
por: Sun, Yihua, et al.
Publicado: (2020)

Central base pair flipping and discrimination by PspGI
por: Szczepanowski, Roman H., et al.
Publicado: (2008)

The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers
por: Piasecka, Anna, et al.
Publicado: (2017)

Competition between electrostatic interactions and halogen bonding in the protein–ligand system: structural and thermodynamic studies of 5,6-dibromobenzotriazole-hCK2α complexes
por: Winiewska-Szajewska, Maria, et al.
Publicado: (2022)

Oxidation of phosphorothioate DNA modifications leads to lethal genomic instability
por: Kellner, Stefanie, et al.
Publicado: (2017)

Engineering Infrequent DNA Nicking Endonuclease by Fusion of a BamHI Cleavage-Deficient Mutant and a DNA Nicking Domain
por: Xu, Shuang-yong
Publicado: (2022)

DNA phosphorothioate modification—a new multi-functional epigenetic system in bacteria
por: Wang, Lianrong, et al.
Publicado: (2018)

DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target
por: Shen, Betty W., et al.
Publicado: (2017)

DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans
por: Dai, Daofeng, et al.
Publicado: (2016)

Crystal structure of human Acinus RNA recognition motif domain
por: Fernandes, Humberto, et al.
Publicado: (2018)

Structural basis of the methylation specificity of R.DpnI
por: Mierzejewska, Karolina, et al.
Publicado: (2014)

Solitary restriction endonucleases in prokaryotic genomes
por: Ershova, Anna S., et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_ir140140u56kr54u9b67ngigb6