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Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms

In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active marine bacteria was performed by combining the DNA stain SYBR Green with detecti...

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Detalles Bibliográficos
Autores principales:	Lindivat, Mathilde, Larsen, Aud, Hess-Erga, Ole Kristian, Bratbak, Gunnar, Hoell, Ingunn Alne
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461810/ https://www.ncbi.nlm.nih.gov/pubmed/33013733 http://dx.doi.org/10.3389/fmicb.2020.01929

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