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Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant
Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to d...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462534/ https://www.ncbi.nlm.nih.gov/pubmed/32905045 http://dx.doi.org/10.1016/j.xcrm.2020.100099 |
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| author | Mlcochova, Petra Collier, Dami Ritchie, Allyson Assennato, Sonny M. Hosmillo, Myra Goel, Neha Meng, Bo Chatterjee, Krishna Mendoza, Vivien Temperton, Nigel Kiss, Leo James, Leo C. Ciazynska, Katarzyna A. Xiong, Xiaoli Briggs, John A.G. Nathan, James A. Mescia, Federica Bergamaschi, Laura Zhang, Hongyi Barmpounakis, Petros Demeris, Nikos Skells, Richard Lyons, Paul A. Bradley, John Baker, Steven Allain, Jean Pierre Smith, Kenneth G.C. Bousfield, Rachel Wilson, Michael Sparkes, Dominic Amoroso, Glenn Gkrania-Klotsas, Effrosyni Hardwick, Susie Boyle, Adrian Goodfellow, Ian Gupta, Ravindra K. |
| author_facet | Mlcochova, Petra Collier, Dami Ritchie, Allyson Assennato, Sonny M. Hosmillo, Myra Goel, Neha Meng, Bo Chatterjee, Krishna Mendoza, Vivien Temperton, Nigel Kiss, Leo James, Leo C. Ciazynska, Katarzyna A. Xiong, Xiaoli Briggs, John A.G. Nathan, James A. Mescia, Federica Bergamaschi, Laura Zhang, Hongyi Barmpounakis, Petros Demeris, Nikos Skells, Richard Lyons, Paul A. Bradley, John Baker, Steven Allain, Jean Pierre Smith, Kenneth G.C. Bousfield, Rachel Wilson, Michael Sparkes, Dominic Amoroso, Glenn Gkrania-Klotsas, Effrosyni Hardwick, Susie Boyle, Adrian Goodfellow, Ian Gupta, Ravindra K. |
| author_sort | Mlcochova, Petra |
| collection | PubMed |
| description | Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity. |
| format | Online Article Text |
| id | pubmed-7462534 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-74625342020-09-02 Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant Mlcochova, Petra Collier, Dami Ritchie, Allyson Assennato, Sonny M. Hosmillo, Myra Goel, Neha Meng, Bo Chatterjee, Krishna Mendoza, Vivien Temperton, Nigel Kiss, Leo James, Leo C. Ciazynska, Katarzyna A. Xiong, Xiaoli Briggs, John A.G. Nathan, James A. Mescia, Federica Bergamaschi, Laura Zhang, Hongyi Barmpounakis, Petros Demeris, Nikos Skells, Richard Lyons, Paul A. Bradley, John Baker, Steven Allain, Jean Pierre Smith, Kenneth G.C. Bousfield, Rachel Wilson, Michael Sparkes, Dominic Amoroso, Glenn Gkrania-Klotsas, Effrosyni Hardwick, Susie Boyle, Adrian Goodfellow, Ian Gupta, Ravindra K. Cell Rep Med Report Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity. Elsevier 2020-09-01 /pmc/articles/PMC7462534/ /pubmed/32905045 http://dx.doi.org/10.1016/j.xcrm.2020.100099 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Report Mlcochova, Petra Collier, Dami Ritchie, Allyson Assennato, Sonny M. Hosmillo, Myra Goel, Neha Meng, Bo Chatterjee, Krishna Mendoza, Vivien Temperton, Nigel Kiss, Leo James, Leo C. Ciazynska, Katarzyna A. Xiong, Xiaoli Briggs, John A.G. Nathan, James A. Mescia, Federica Bergamaschi, Laura Zhang, Hongyi Barmpounakis, Petros Demeris, Nikos Skells, Richard Lyons, Paul A. Bradley, John Baker, Steven Allain, Jean Pierre Smith, Kenneth G.C. Bousfield, Rachel Wilson, Michael Sparkes, Dominic Amoroso, Glenn Gkrania-Klotsas, Effrosyni Hardwick, Susie Boyle, Adrian Goodfellow, Ian Gupta, Ravindra K. Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title_full | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title_fullStr | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title_full_unstemmed | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title_short | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
| title_sort | combined point-of-care nucleic acid and antibody testing for sars-cov-2 following emergence of d614g spike variant |
| topic | Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462534/ https://www.ncbi.nlm.nih.gov/pubmed/32905045 http://dx.doi.org/10.1016/j.xcrm.2020.100099 |
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