(1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei

KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the (1)H, (13)C and (15)N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4...

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Autores principales: Ludzia, Patryk, Akiyoshi, Bungo, Redfield, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462909/
https://www.ncbi.nlm.nih.gov/pubmed/32696260
http://dx.doi.org/10.1007/s12104-020-09968-1
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author Ludzia, Patryk
Akiyoshi, Bungo
Redfield, Christina
author_facet Ludzia, Patryk
Akiyoshi, Bungo
Redfield, Christina
author_sort Ludzia, Patryk
collection PubMed
description KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the (1)H, (13)C and (15)N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4(115–343)) from Trypanosoma brucei. These assignments provide the starting point for detailed investigations of the structure, dynamics and interactions of the microtubule-binding region of KKT4. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12104-020-09968-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-74629092020-09-11 (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei Ludzia, Patryk Akiyoshi, Bungo Redfield, Christina Biomol NMR Assign Article KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the (1)H, (13)C and (15)N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4(115–343)) from Trypanosoma brucei. These assignments provide the starting point for detailed investigations of the structure, dynamics and interactions of the microtubule-binding region of KKT4. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12104-020-09968-1) contains supplementary material, which is available to authorized users. Springer Netherlands 2020-07-21 2020 /pmc/articles/PMC7462909/ /pubmed/32696260 http://dx.doi.org/10.1007/s12104-020-09968-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ludzia, Patryk
Akiyoshi, Bungo
Redfield, Christina
(1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title_full (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title_fullStr (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title_full_unstemmed (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title_short (1)H, (13)C and (15)N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
title_sort (1)h, (13)c and (15)n resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein kkt4 from trypanosoma brucei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462909/
https://www.ncbi.nlm.nih.gov/pubmed/32696260
http://dx.doi.org/10.1007/s12104-020-09968-1
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