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The effect of fluoride on enamel and dentin formation in the uremic rat incisor
Renal impairment in children is associated with tooth defects that include enamel pitting and hypoplasia. However, the specific effects of uremia on tooth formation are not known. In this study, we used rat mandibular incisors, which continuously erupt and contain all stages of tooth formation, to c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462915/ https://www.ncbi.nlm.nih.gov/pubmed/18563453 http://dx.doi.org/10.1007/s00467-008-0890-2 |
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author | Lyaruu, Donacian M. Bronckers, Antonius L. J. J. Santos, Fernando Mathias, Robert DenBesten, Pamela |
author_facet | Lyaruu, Donacian M. Bronckers, Antonius L. J. J. Santos, Fernando Mathias, Robert DenBesten, Pamela |
author_sort | Lyaruu, Donacian M. |
collection | PubMed |
description | Renal impairment in children is associated with tooth defects that include enamel pitting and hypoplasia. However, the specific effects of uremia on tooth formation are not known. In this study, we used rat mandibular incisors, which continuously erupt and contain all stages of tooth formation, to characterize the effects of uremia on tooth formation. We also tested the hypothesis that uremia aggravates the fluoride (F)-induced changes in developing teeth. Rats were subjected to a two-stage 5/6 nephrectomy or sham operation and then exposed to 0 (control) or 50 ppm NaF in drinking water for 14 days. The effects of these treatments on food intake, body growth rate, and biochemical serum parameters for renal function and calcium metabolism were monitored. Nephrectomy reduced food intake and weight gain. Intake of F by nephrectomized rats increased plasma F levels twofold and further decreased food intake and body weight gain. Uremia affected formation of dentin and enamel and was more extensive than the effect of F alone. Uremia also significantly increased predentin width and induced deposition of large amounts of osteodentin-like matrix-containing cells in the pulp chamber. In enamel formation, the cells most sensitive to uremia were the transitional-stage ameloblasts. These data demonstrate that intake of F by rats with reduced renal function impairs F clearance from the plasma and aggravates the already negative effects of uremia on incisor tooth development. |
format | Online Article Text |
id | pubmed-7462915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-74629152020-09-11 The effect of fluoride on enamel and dentin formation in the uremic rat incisor Lyaruu, Donacian M. Bronckers, Antonius L. J. J. Santos, Fernando Mathias, Robert DenBesten, Pamela Pediatr Nephrol Original Article Renal impairment in children is associated with tooth defects that include enamel pitting and hypoplasia. However, the specific effects of uremia on tooth formation are not known. In this study, we used rat mandibular incisors, which continuously erupt and contain all stages of tooth formation, to characterize the effects of uremia on tooth formation. We also tested the hypothesis that uremia aggravates the fluoride (F)-induced changes in developing teeth. Rats were subjected to a two-stage 5/6 nephrectomy or sham operation and then exposed to 0 (control) or 50 ppm NaF in drinking water for 14 days. The effects of these treatments on food intake, body growth rate, and biochemical serum parameters for renal function and calcium metabolism were monitored. Nephrectomy reduced food intake and weight gain. Intake of F by nephrectomized rats increased plasma F levels twofold and further decreased food intake and body weight gain. Uremia affected formation of dentin and enamel and was more extensive than the effect of F alone. Uremia also significantly increased predentin width and induced deposition of large amounts of osteodentin-like matrix-containing cells in the pulp chamber. In enamel formation, the cells most sensitive to uremia were the transitional-stage ameloblasts. These data demonstrate that intake of F by rats with reduced renal function impairs F clearance from the plasma and aggravates the already negative effects of uremia on incisor tooth development. Springer Berlin Heidelberg 2008-11-01 2008 /pmc/articles/PMC7462915/ /pubmed/18563453 http://dx.doi.org/10.1007/s00467-008-0890-2 Text en © The Author(s) 2008 https://creativecommons.org/licenses/by-nc/2.0/Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0 (https://creativecommons.org/licenses/by-nc/2.0/) ), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Article Lyaruu, Donacian M. Bronckers, Antonius L. J. J. Santos, Fernando Mathias, Robert DenBesten, Pamela The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title | The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title_full | The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title_fullStr | The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title_full_unstemmed | The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title_short | The effect of fluoride on enamel and dentin formation in the uremic rat incisor |
title_sort | effect of fluoride on enamel and dentin formation in the uremic rat incisor |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462915/ https://www.ncbi.nlm.nih.gov/pubmed/18563453 http://dx.doi.org/10.1007/s00467-008-0890-2 |
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