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Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways
Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen in intensive care units and causes infections most often after surgeries in immune-compromised patients such as those undergoing chemotherapy. Outer membrane protein A (OmpA) is the most abundant of the outer membrane p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Microbiological Society of Korea
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463227/ https://www.ncbi.nlm.nih.gov/pubmed/32876914 http://dx.doi.org/10.1007/s12275-020-0235-9 |
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author | Wang, Xin Li, Yan Tang, Xueping Shang, Xueyi Zhao, Zunquan Jiang, Yongqiang Li, Yan |
author_facet | Wang, Xin Li, Yan Tang, Xueping Shang, Xueyi Zhao, Zunquan Jiang, Yongqiang Li, Yan |
author_sort | Wang, Xin |
collection | PubMed |
description | Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen in intensive care units and causes infections most often after surgeries in immune-compromised patients such as those undergoing chemotherapy. Outer membrane protein A (OmpA) is the most abundant of the outer membrane proteins in S. maltophilia. Previous studies on OmpA usually focus on its interaction with the host cells and its role in vaccine development. However, the impact of OmpA on the virulence of S. maltophilia to host cells and the effects on apoptosis remain unclear. In this study, we exposed purified recombinant S. maltophilia OmpA (rOmpA) to HEp-2 cells and investigated the effects of OmpA on epithelial cell apoptosis. Morphologic and flow cytometric analyses revealed that HEp-2 cells stimulated with rOmpA multiple apoptosis features, including nuclear roundness and pyknosis, chromatin aggregation, and phosphatidylserine eversion. We found that rOmpA regulated the protein levels of Bax and Bcl-xL in HEp-2 cells, leading to changes in mitochondria permeability and the release of cytochrome c and apoptosis-inducing factors into the cytoplasm. These subsequently activate the caspase-9/caspase-3 pathway that promote apoptosis. We also observed that rOmpA enhanced the generation of reactive oxygen species and increased intracellular Ca(2+) levels in HEp-2 cells. Collectively, our data suggested that rOmpA induced epithelial cells apoptosis via mi-tochondrial pathways. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s12275-020-0235-9 and is accessible for authorized users. |
format | Online Article Text |
id | pubmed-7463227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Microbiological Society of Korea |
record_format | MEDLINE/PubMed |
spelling | pubmed-74632272020-09-02 Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways Wang, Xin Li, Yan Tang, Xueping Shang, Xueyi Zhao, Zunquan Jiang, Yongqiang Li, Yan J Microbiol Article Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen in intensive care units and causes infections most often after surgeries in immune-compromised patients such as those undergoing chemotherapy. Outer membrane protein A (OmpA) is the most abundant of the outer membrane proteins in S. maltophilia. Previous studies on OmpA usually focus on its interaction with the host cells and its role in vaccine development. However, the impact of OmpA on the virulence of S. maltophilia to host cells and the effects on apoptosis remain unclear. In this study, we exposed purified recombinant S. maltophilia OmpA (rOmpA) to HEp-2 cells and investigated the effects of OmpA on epithelial cell apoptosis. Morphologic and flow cytometric analyses revealed that HEp-2 cells stimulated with rOmpA multiple apoptosis features, including nuclear roundness and pyknosis, chromatin aggregation, and phosphatidylserine eversion. We found that rOmpA regulated the protein levels of Bax and Bcl-xL in HEp-2 cells, leading to changes in mitochondria permeability and the release of cytochrome c and apoptosis-inducing factors into the cytoplasm. These subsequently activate the caspase-9/caspase-3 pathway that promote apoptosis. We also observed that rOmpA enhanced the generation of reactive oxygen species and increased intracellular Ca(2+) levels in HEp-2 cells. Collectively, our data suggested that rOmpA induced epithelial cells apoptosis via mi-tochondrial pathways. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s12275-020-0235-9 and is accessible for authorized users. The Microbiological Society of Korea 2020-09-02 2020 /pmc/articles/PMC7463227/ /pubmed/32876914 http://dx.doi.org/10.1007/s12275-020-0235-9 Text en © The Microbiological Society of Korea 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Wang, Xin Li, Yan Tang, Xueping Shang, Xueyi Zhao, Zunquan Jiang, Yongqiang Li, Yan Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title | Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title_full | Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title_fullStr | Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title_full_unstemmed | Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title_short | Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways |
title_sort | stenotrophomonas maltophilia outer membrane protein a induces epithelial cell apoptosis via mitochondrial pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463227/ https://www.ncbi.nlm.nih.gov/pubmed/32876914 http://dx.doi.org/10.1007/s12275-020-0235-9 |
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