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Evaluating the probability of CRISPR‐based gene drive contaminating another species
The probability D that a given clustered regularly interspaced short palindromic repeats (CRISPR)‐based gene drive element contaminates another, nontarget species can be estimated by the following Drive Risk Assessment Quantitative Estimate (DRAQUE) Equation: [Formula: see text] with hyb = probabili...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463340/ https://www.ncbi.nlm.nih.gov/pubmed/32908593 http://dx.doi.org/10.1111/eva.12939 |
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author | Courtier‐Orgogozo, Virginie Danchin, Antoine Gouyon, Pierre‐Henri Boëte, Christophe |
author_facet | Courtier‐Orgogozo, Virginie Danchin, Antoine Gouyon, Pierre‐Henri Boëte, Christophe |
author_sort | Courtier‐Orgogozo, Virginie |
collection | PubMed |
description | The probability D that a given clustered regularly interspaced short palindromic repeats (CRISPR)‐based gene drive element contaminates another, nontarget species can be estimated by the following Drive Risk Assessment Quantitative Estimate (DRAQUE) Equation: [Formula: see text] with hyb = probability of hybridization between the target species and a nontarget species; transf = probability of horizontal transfer of a piece of DNA containing the gene drive cassette from the target species to a nontarget species (with no hybridization); express = probability that the Cas9 and guide RNA genes are expressed; cut = probability that the CRISPR‐guide RNA recognizes and cuts at a DNA site in the new host; flank = probability that the gene drive cassette inserts at the cut site; immune = probability that the immune system does not reject Cas9‐expressing cells; nonextinct = probability of invasion of the drive within the population. We discuss and estimate each of the seven parameters of the equation, with particular emphasis on possible transfers within insects, and between rodents and humans. We conclude from current data that the probability of a gene drive cassette to contaminate another species is not insignificant. We propose strategies to reduce this risk and call for more work on estimating all the parameters of the formula. |
format | Online Article Text |
id | pubmed-7463340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74633402020-09-08 Evaluating the probability of CRISPR‐based gene drive contaminating another species Courtier‐Orgogozo, Virginie Danchin, Antoine Gouyon, Pierre‐Henri Boëte, Christophe Evol Appl Original Articles The probability D that a given clustered regularly interspaced short palindromic repeats (CRISPR)‐based gene drive element contaminates another, nontarget species can be estimated by the following Drive Risk Assessment Quantitative Estimate (DRAQUE) Equation: [Formula: see text] with hyb = probability of hybridization between the target species and a nontarget species; transf = probability of horizontal transfer of a piece of DNA containing the gene drive cassette from the target species to a nontarget species (with no hybridization); express = probability that the Cas9 and guide RNA genes are expressed; cut = probability that the CRISPR‐guide RNA recognizes and cuts at a DNA site in the new host; flank = probability that the gene drive cassette inserts at the cut site; immune = probability that the immune system does not reject Cas9‐expressing cells; nonextinct = probability of invasion of the drive within the population. We discuss and estimate each of the seven parameters of the equation, with particular emphasis on possible transfers within insects, and between rodents and humans. We conclude from current data that the probability of a gene drive cassette to contaminate another species is not insignificant. We propose strategies to reduce this risk and call for more work on estimating all the parameters of the formula. John Wiley and Sons Inc. 2020-04-17 /pmc/articles/PMC7463340/ /pubmed/32908593 http://dx.doi.org/10.1111/eva.12939 Text en © 2020 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Courtier‐Orgogozo, Virginie Danchin, Antoine Gouyon, Pierre‐Henri Boëte, Christophe Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title | Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title_full | Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title_fullStr | Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title_full_unstemmed | Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title_short | Evaluating the probability of CRISPR‐based gene drive contaminating another species |
title_sort | evaluating the probability of crispr‐based gene drive contaminating another species |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463340/ https://www.ncbi.nlm.nih.gov/pubmed/32908593 http://dx.doi.org/10.1111/eva.12939 |
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