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Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing

The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular het...

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Detalles Bibliográficos
Autores principales: Gupta, Rishikesh Kumar, Kuznicki, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463515/
https://www.ncbi.nlm.nih.gov/pubmed/32707839
http://dx.doi.org/10.3390/cells9081751
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author Gupta, Rishikesh Kumar
Kuznicki, Jacek
author_facet Gupta, Rishikesh Kumar
Kuznicki, Jacek
author_sort Gupta, Rishikesh Kumar
collection PubMed
description The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular heterogeneity that is present in cell populations with very high resolution of the transcriptomic landscape. We summarize scRNA-seq findings and applications of this technology to identify cell types, activity, and other features that are important for the function of different bodily organs. We discuss future directions for scRNA-seq techniques that can link gene expression, protein expression, cellular function, and their roles in pathology. We speculate on how the field could develop beyond its present limitations (e.g., performing scRNA-seq in situ and in vivo). Finally, we discuss the integration of machine learning and artificial intelligence with cutting-edge scRNA-seq technology, which could provide a strong basis for designing precision medicine and targeted therapy in the future.
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spelling pubmed-74635152020-09-04 Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing Gupta, Rishikesh Kumar Kuznicki, Jacek Cells Review The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular heterogeneity that is present in cell populations with very high resolution of the transcriptomic landscape. We summarize scRNA-seq findings and applications of this technology to identify cell types, activity, and other features that are important for the function of different bodily organs. We discuss future directions for scRNA-seq techniques that can link gene expression, protein expression, cellular function, and their roles in pathology. We speculate on how the field could develop beyond its present limitations (e.g., performing scRNA-seq in situ and in vivo). Finally, we discuss the integration of machine learning and artificial intelligence with cutting-edge scRNA-seq technology, which could provide a strong basis for designing precision medicine and targeted therapy in the future. MDPI 2020-07-22 /pmc/articles/PMC7463515/ /pubmed/32707839 http://dx.doi.org/10.3390/cells9081751 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gupta, Rishikesh Kumar
Kuznicki, Jacek
Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title_full Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title_fullStr Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title_full_unstemmed Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title_short Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
title_sort biological and medical importance of cellular heterogeneity deciphered by single-cell rna sequencing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463515/
https://www.ncbi.nlm.nih.gov/pubmed/32707839
http://dx.doi.org/10.3390/cells9081751
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