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Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing
The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular het...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463515/ https://www.ncbi.nlm.nih.gov/pubmed/32707839 http://dx.doi.org/10.3390/cells9081751 |
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author | Gupta, Rishikesh Kumar Kuznicki, Jacek |
author_facet | Gupta, Rishikesh Kumar Kuznicki, Jacek |
author_sort | Gupta, Rishikesh Kumar |
collection | PubMed |
description | The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular heterogeneity that is present in cell populations with very high resolution of the transcriptomic landscape. We summarize scRNA-seq findings and applications of this technology to identify cell types, activity, and other features that are important for the function of different bodily organs. We discuss future directions for scRNA-seq techniques that can link gene expression, protein expression, cellular function, and their roles in pathology. We speculate on how the field could develop beyond its present limitations (e.g., performing scRNA-seq in situ and in vivo). Finally, we discuss the integration of machine learning and artificial intelligence with cutting-edge scRNA-seq technology, which could provide a strong basis for designing precision medicine and targeted therapy in the future. |
format | Online Article Text |
id | pubmed-7463515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74635152020-09-04 Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing Gupta, Rishikesh Kumar Kuznicki, Jacek Cells Review The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq), which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies (e.g., cancer and Alzheimer’s disease). We outline scRNA-seq techniques that are suitable for investigating cellular heterogeneity that is present in cell populations with very high resolution of the transcriptomic landscape. We summarize scRNA-seq findings and applications of this technology to identify cell types, activity, and other features that are important for the function of different bodily organs. We discuss future directions for scRNA-seq techniques that can link gene expression, protein expression, cellular function, and their roles in pathology. We speculate on how the field could develop beyond its present limitations (e.g., performing scRNA-seq in situ and in vivo). Finally, we discuss the integration of machine learning and artificial intelligence with cutting-edge scRNA-seq technology, which could provide a strong basis for designing precision medicine and targeted therapy in the future. MDPI 2020-07-22 /pmc/articles/PMC7463515/ /pubmed/32707839 http://dx.doi.org/10.3390/cells9081751 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Gupta, Rishikesh Kumar Kuznicki, Jacek Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title | Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title_full | Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title_fullStr | Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title_full_unstemmed | Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title_short | Biological and Medical Importance of Cellular Heterogeneity Deciphered by Single-Cell RNA Sequencing |
title_sort | biological and medical importance of cellular heterogeneity deciphered by single-cell rna sequencing |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463515/ https://www.ncbi.nlm.nih.gov/pubmed/32707839 http://dx.doi.org/10.3390/cells9081751 |
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