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Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter

Variation in some caprine keratin-associated protein (KAP) genes has been associated with cashmere fiber traits, but many KAP genes remain unidentified in goats. In this study, we confirm the identification of a KAP27-1 gene (KRTAP27-1) and describe its effect on cashmere traits in 248 Longdong cash...

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Autores principales: Zhao, Mengli, Zhou, Huitong, Luo, Yuzhu, Wang, Jiqing, Hu, Jiang, Liu, Xiu, Li, Shaobin, Hao, Zhiyun, Jin, Xiayang, Song, Yize, Wu, Xinmiao, Hu, Liyan, Hickford, Jon G. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463587/
https://www.ncbi.nlm.nih.gov/pubmed/32823629
http://dx.doi.org/10.3390/genes11080934
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author Zhao, Mengli
Zhou, Huitong
Luo, Yuzhu
Wang, Jiqing
Hu, Jiang
Liu, Xiu
Li, Shaobin
Hao, Zhiyun
Jin, Xiayang
Song, Yize
Wu, Xinmiao
Hu, Liyan
Hickford, Jon G. H.
author_facet Zhao, Mengli
Zhou, Huitong
Luo, Yuzhu
Wang, Jiqing
Hu, Jiang
Liu, Xiu
Li, Shaobin
Hao, Zhiyun
Jin, Xiayang
Song, Yize
Wu, Xinmiao
Hu, Liyan
Hickford, Jon G. H.
author_sort Zhao, Mengli
collection PubMed
description Variation in some caprine keratin-associated protein (KAP) genes has been associated with cashmere fiber traits, but many KAP genes remain unidentified in goats. In this study, we confirm the identification of a KAP27-1 gene (KRTAP27-1) and describe its effect on cashmere traits in 248 Longdong cashmere goats. A polymerase chain reaction–single strand conformation polymorphism (PCR-SSCP) analysis was used to screen for sequence variation in this gene, and three sequence variants (named A to C) were found. These sequences have the highest similarity (77% identity) to a human KRTAP27-1 sequence, while sharing some homology with a predicted caprine KRTAP27-1 sequence ENSCHIG00000023347 in the goat genome construct (ARS1:CM004562.1) at chromosome 1 position 3,966,193–3,973,677 in the forward strand. There were two single nucleotide polymorphisms (SNPs) detected in the coding sequence, including one nonsynonymous SNP (c.413C/T; p.Ala138Val) and one synonymous SNP (c.495C/T). The C variant differed from A and B at c.413C/T, having cytosine in its nucleotide sequence, while the B variant differed from A and C at c.495C/T, having thymine in its nucleotide sequence. Goats of the genotypes AB and BB produced cashmere fibers of higher mean fiber diameter (MFD) than goats of genotype AA, but no difference in MFD was detected between the AB and BB goats. These results suggest that B is associated with increased MFD. Expression of the caprine KRTAP27-1 sequence was predominantly detected in the skin tissue of goats but not or only weakly detected in other tissues, including longissimus dorsi muscle, heart, kidney, liver, lung and spleen.
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spelling pubmed-74635872020-09-02 Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter Zhao, Mengli Zhou, Huitong Luo, Yuzhu Wang, Jiqing Hu, Jiang Liu, Xiu Li, Shaobin Hao, Zhiyun Jin, Xiayang Song, Yize Wu, Xinmiao Hu, Liyan Hickford, Jon G. H. Genes (Basel) Article Variation in some caprine keratin-associated protein (KAP) genes has been associated with cashmere fiber traits, but many KAP genes remain unidentified in goats. In this study, we confirm the identification of a KAP27-1 gene (KRTAP27-1) and describe its effect on cashmere traits in 248 Longdong cashmere goats. A polymerase chain reaction–single strand conformation polymorphism (PCR-SSCP) analysis was used to screen for sequence variation in this gene, and three sequence variants (named A to C) were found. These sequences have the highest similarity (77% identity) to a human KRTAP27-1 sequence, while sharing some homology with a predicted caprine KRTAP27-1 sequence ENSCHIG00000023347 in the goat genome construct (ARS1:CM004562.1) at chromosome 1 position 3,966,193–3,973,677 in the forward strand. There were two single nucleotide polymorphisms (SNPs) detected in the coding sequence, including one nonsynonymous SNP (c.413C/T; p.Ala138Val) and one synonymous SNP (c.495C/T). The C variant differed from A and B at c.413C/T, having cytosine in its nucleotide sequence, while the B variant differed from A and C at c.495C/T, having thymine in its nucleotide sequence. Goats of the genotypes AB and BB produced cashmere fibers of higher mean fiber diameter (MFD) than goats of genotype AA, but no difference in MFD was detected between the AB and BB goats. These results suggest that B is associated with increased MFD. Expression of the caprine KRTAP27-1 sequence was predominantly detected in the skin tissue of goats but not or only weakly detected in other tissues, including longissimus dorsi muscle, heart, kidney, liver, lung and spleen. MDPI 2020-08-13 /pmc/articles/PMC7463587/ /pubmed/32823629 http://dx.doi.org/10.3390/genes11080934 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Mengli
Zhou, Huitong
Luo, Yuzhu
Wang, Jiqing
Hu, Jiang
Liu, Xiu
Li, Shaobin
Hao, Zhiyun
Jin, Xiayang
Song, Yize
Wu, Xinmiao
Hu, Liyan
Hickford, Jon G. H.
Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title_full Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title_fullStr Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title_full_unstemmed Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title_short Variation in the Caprine Keratin-Associated Protein 27-1 Gene is Associated with Cashmere Fiber Diameter
title_sort variation in the caprine keratin-associated protein 27-1 gene is associated with cashmere fiber diameter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463587/
https://www.ncbi.nlm.nih.gov/pubmed/32823629
http://dx.doi.org/10.3390/genes11080934
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