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Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties

Topographical modification of the dental implant surface is one of the main topics for the improvement of the material, however, the roughened surface has some risks for peri-implantitis. A hydrothermal treatment (HT) of titanium with calcium chloride solution was reported to improve osseointegratio...

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Autores principales: Haraguchi, Takuya, Ayukawa, Yasunori, Shibata, Yukie, Takeshita, Toru, Atsuta, Ikiru, Ogino, Yoichiro, Yasunami, Noriyuki, Yamashita, Yoshihisa, Koyano, Kiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463643/
https://www.ncbi.nlm.nih.gov/pubmed/32823609
http://dx.doi.org/10.3390/jcm9082627
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author Haraguchi, Takuya
Ayukawa, Yasunori
Shibata, Yukie
Takeshita, Toru
Atsuta, Ikiru
Ogino, Yoichiro
Yasunami, Noriyuki
Yamashita, Yoshihisa
Koyano, Kiyoshi
author_facet Haraguchi, Takuya
Ayukawa, Yasunori
Shibata, Yukie
Takeshita, Toru
Atsuta, Ikiru
Ogino, Yoichiro
Yasunami, Noriyuki
Yamashita, Yoshihisa
Koyano, Kiyoshi
author_sort Haraguchi, Takuya
collection PubMed
description Topographical modification of the dental implant surface is one of the main topics for the improvement of the material, however, the roughened surface has some risks for peri-implantitis. A hydrothermal treatment (HT) of titanium with calcium chloride solution was reported to improve osseointegration and soft tissue sealing without changing the surface topography; however, its mechanism is unclear. We herewith investigated the interaction between extracellular matrix (ECM) protein and HT titanium. Furthermore, we also clarified the bacterial interaction. We employed two kinds of HT, HT with water (DW-HT) and HT with calcium chloride solution (Ca-HT). As a result, the adsorptions of both laminin-332 and osteopontin onto the Ca-HT surface were enhanced. In contrast, the adsorption of albumin, which was reported to have no cell adhesion capacity, was not influenced by Ca-HT. Osteoblast adhesion onto Ca-HT was also enhanced. Although Ca-HT was reported to enhance both epithelial cell attachment strength and in vivo peri-implant epithelial bonding, the number of epithelial cell attachment was not increased even after HT. Ca-HT had no impact in the adhesion of Streptococcus gordonii. These results suggest that Ca-HT enhances cell adhesion onto titanium without increasing bacterial adhesion, and the improvement of ECM protein adsorption is supposed to contribute to cell adhesion.
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spelling pubmed-74636432020-09-02 Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties Haraguchi, Takuya Ayukawa, Yasunori Shibata, Yukie Takeshita, Toru Atsuta, Ikiru Ogino, Yoichiro Yasunami, Noriyuki Yamashita, Yoshihisa Koyano, Kiyoshi J Clin Med Article Topographical modification of the dental implant surface is one of the main topics for the improvement of the material, however, the roughened surface has some risks for peri-implantitis. A hydrothermal treatment (HT) of titanium with calcium chloride solution was reported to improve osseointegration and soft tissue sealing without changing the surface topography; however, its mechanism is unclear. We herewith investigated the interaction between extracellular matrix (ECM) protein and HT titanium. Furthermore, we also clarified the bacterial interaction. We employed two kinds of HT, HT with water (DW-HT) and HT with calcium chloride solution (Ca-HT). As a result, the adsorptions of both laminin-332 and osteopontin onto the Ca-HT surface were enhanced. In contrast, the adsorption of albumin, which was reported to have no cell adhesion capacity, was not influenced by Ca-HT. Osteoblast adhesion onto Ca-HT was also enhanced. Although Ca-HT was reported to enhance both epithelial cell attachment strength and in vivo peri-implant epithelial bonding, the number of epithelial cell attachment was not increased even after HT. Ca-HT had no impact in the adhesion of Streptococcus gordonii. These results suggest that Ca-HT enhances cell adhesion onto titanium without increasing bacterial adhesion, and the improvement of ECM protein adsorption is supposed to contribute to cell adhesion. MDPI 2020-08-13 /pmc/articles/PMC7463643/ /pubmed/32823609 http://dx.doi.org/10.3390/jcm9082627 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haraguchi, Takuya
Ayukawa, Yasunori
Shibata, Yukie
Takeshita, Toru
Atsuta, Ikiru
Ogino, Yoichiro
Yasunami, Noriyuki
Yamashita, Yoshihisa
Koyano, Kiyoshi
Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title_full Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title_fullStr Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title_full_unstemmed Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title_short Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
title_sort effect of calcium chloride hydrothermal treatment of titanium on protein, cellular, and bacterial adhesion properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463643/
https://www.ncbi.nlm.nih.gov/pubmed/32823609
http://dx.doi.org/10.3390/jcm9082627
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