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Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis

The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from stud...

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Autores principales: Wiener, Dominique J., Groch, Kátia R., Brunner, Magdalena A.T., Leeb, Tosso, Jagannathan, Vidhya, Welle, Monika M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463739/
https://www.ncbi.nlm.nih.gov/pubmed/32759649
http://dx.doi.org/10.3390/genes11080884
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author Wiener, Dominique J.
Groch, Kátia R.
Brunner, Magdalena A.T.
Leeb, Tosso
Jagannathan, Vidhya
Welle, Monika M.
author_facet Wiener, Dominique J.
Groch, Kátia R.
Brunner, Magdalena A.T.
Leeb, Tosso
Jagannathan, Vidhya
Welle, Monika M.
author_sort Wiener, Dominique J.
collection PubMed
description The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15. The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states.
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spelling pubmed-74637392020-09-02 Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis Wiener, Dominique J. Groch, Kátia R. Brunner, Magdalena A.T. Leeb, Tosso Jagannathan, Vidhya Welle, Monika M. Genes (Basel) Article The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15. The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states. MDPI 2020-08-04 /pmc/articles/PMC7463739/ /pubmed/32759649 http://dx.doi.org/10.3390/genes11080884 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wiener, Dominique J.
Groch, Kátia R.
Brunner, Magdalena A.T.
Leeb, Tosso
Jagannathan, Vidhya
Welle, Monika M.
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title_full Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title_fullStr Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title_full_unstemmed Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title_short Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
title_sort transcriptome profiling and differential gene expression in canine microdissected anagen and telogen hair follicles and interfollicular epidermis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463739/
https://www.ncbi.nlm.nih.gov/pubmed/32759649
http://dx.doi.org/10.3390/genes11080884
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