Cargando…

Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance

In this work, the metabolic elicitors extracted from the beneficial rhizobacterium Pseudomonas fluorescens N 21.4 were sequentially fragmented by vacuum liquid chromatography to isolate, purify and identify the compounds responsible for the extraordinary capacities of this strain to induce systemic...

Descripción completa

Detalles Bibliográficos
Autores principales: Martin-Rivilla, Helena, Gutierrez-Mañero, F. Javier, Gradillas, Ana, P. Navarro, Miguel O., Andrade, Galdino, Lucas, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463883/
https://www.ncbi.nlm.nih.gov/pubmed/32806693
http://dx.doi.org/10.3390/plants9081020
_version_ 1783577236376387584
author Martin-Rivilla, Helena
Gutierrez-Mañero, F. Javier
Gradillas, Ana
P. Navarro, Miguel O.
Andrade, Galdino
Lucas, José A.
author_facet Martin-Rivilla, Helena
Gutierrez-Mañero, F. Javier
Gradillas, Ana
P. Navarro, Miguel O.
Andrade, Galdino
Lucas, José A.
author_sort Martin-Rivilla, Helena
collection PubMed
description In this work, the metabolic elicitors extracted from the beneficial rhizobacterium Pseudomonas fluorescens N 21.4 were sequentially fragmented by vacuum liquid chromatography to isolate, purify and identify the compounds responsible for the extraordinary capacities of this strain to induce systemic resistance and to elicit secondary defensive metabolism in diverse plant species. To check if the fractions sequentially obtained were able to increase the synthesis of isoflavones and if, therefore, they still maintained the eliciting capacity of the live strain, rapid and controlled experiments were done with soybean seeds. The optimal action concentration of the fractions was established and all of them elicited isoflavone secondary metabolism—the fractions that had been extracted with n-hexane being more effective. The purest fraction was the one with the highest eliciting capacity and was also tested in Arabidopsis thaliana seedlings to induce systemic resistance against the pathogen Pseudomonas syringae pv. tomato DC 3000. This fraction was then analyzed by UHPLC/ESI–QTOF–MS, and an alkaloid, two amino lipids, three arylalkylamines and a terpenoid were tentatively identified. These identified compounds could be part of commercial plant inoculants of biological and sustainable origin to be applied in crops, due to their potential to enhance the plant immune response and since many of them have putative antibiotic and/or antifungal potential.
format Online
Article
Text
id pubmed-7463883
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74638832020-09-04 Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance Martin-Rivilla, Helena Gutierrez-Mañero, F. Javier Gradillas, Ana P. Navarro, Miguel O. Andrade, Galdino Lucas, José A. Plants (Basel) Article In this work, the metabolic elicitors extracted from the beneficial rhizobacterium Pseudomonas fluorescens N 21.4 were sequentially fragmented by vacuum liquid chromatography to isolate, purify and identify the compounds responsible for the extraordinary capacities of this strain to induce systemic resistance and to elicit secondary defensive metabolism in diverse plant species. To check if the fractions sequentially obtained were able to increase the synthesis of isoflavones and if, therefore, they still maintained the eliciting capacity of the live strain, rapid and controlled experiments were done with soybean seeds. The optimal action concentration of the fractions was established and all of them elicited isoflavone secondary metabolism—the fractions that had been extracted with n-hexane being more effective. The purest fraction was the one with the highest eliciting capacity and was also tested in Arabidopsis thaliana seedlings to induce systemic resistance against the pathogen Pseudomonas syringae pv. tomato DC 3000. This fraction was then analyzed by UHPLC/ESI–QTOF–MS, and an alkaloid, two amino lipids, three arylalkylamines and a terpenoid were tentatively identified. These identified compounds could be part of commercial plant inoculants of biological and sustainable origin to be applied in crops, due to their potential to enhance the plant immune response and since many of them have putative antibiotic and/or antifungal potential. MDPI 2020-08-12 /pmc/articles/PMC7463883/ /pubmed/32806693 http://dx.doi.org/10.3390/plants9081020 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martin-Rivilla, Helena
Gutierrez-Mañero, F. Javier
Gradillas, Ana
P. Navarro, Miguel O.
Andrade, Galdino
Lucas, José A.
Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title_full Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title_fullStr Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title_full_unstemmed Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title_short Identifying the Compounds of the Metabolic Elicitors of Pseudomonas fluorescens N 21.4 Responsible for Their Ability to Induce Plant Resistance
title_sort identifying the compounds of the metabolic elicitors of pseudomonas fluorescens n 21.4 responsible for their ability to induce plant resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463883/
https://www.ncbi.nlm.nih.gov/pubmed/32806693
http://dx.doi.org/10.3390/plants9081020
work_keys_str_mv AT martinrivillahelena identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance
AT gutierrezmanerofjavier identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance
AT gradillasana identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance
AT pnavarromiguelo identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance
AT andradegaldino identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance
AT lucasjosea identifyingthecompoundsofthemetabolicelicitorsofpseudomonasfluorescensn214responsiblefortheirabilitytoinduceplantresistance