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RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will b...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463892/ https://www.ncbi.nlm.nih.gov/pubmed/32731493 http://dx.doi.org/10.3390/mi11080729 |
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author | Yadav, Sharda Kashaninejad, Navid Nguyen, Nam-Trung |
author_facet | Yadav, Sharda Kashaninejad, Navid Nguyen, Nam-Trung |
author_sort | Yadav, Sharda |
collection | PubMed |
description | Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients. |
format | Online Article Text |
id | pubmed-7463892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74638922020-09-04 RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation Yadav, Sharda Kashaninejad, Navid Nguyen, Nam-Trung Micromachines (Basel) Article Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients. MDPI 2020-07-28 /pmc/articles/PMC7463892/ /pubmed/32731493 http://dx.doi.org/10.3390/mi11080729 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yadav, Sharda Kashaninejad, Navid Nguyen, Nam-Trung RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title_full | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title_fullStr | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title_full_unstemmed | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title_short | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
title_sort | rhoa and rac1 in liver cancer cells: induction of overexpression using mechanical stimulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463892/ https://www.ncbi.nlm.nih.gov/pubmed/32731493 http://dx.doi.org/10.3390/mi11080729 |
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