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RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation

Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will b...

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Autores principales: Yadav, Sharda, Kashaninejad, Navid, Nguyen, Nam-Trung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463892/
https://www.ncbi.nlm.nih.gov/pubmed/32731493
http://dx.doi.org/10.3390/mi11080729
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author Yadav, Sharda
Kashaninejad, Navid
Nguyen, Nam-Trung
author_facet Yadav, Sharda
Kashaninejad, Navid
Nguyen, Nam-Trung
author_sort Yadav, Sharda
collection PubMed
description Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients.
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spelling pubmed-74638922020-09-04 RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation Yadav, Sharda Kashaninejad, Navid Nguyen, Nam-Trung Micromachines (Basel) Article Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients. MDPI 2020-07-28 /pmc/articles/PMC7463892/ /pubmed/32731493 http://dx.doi.org/10.3390/mi11080729 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yadav, Sharda
Kashaninejad, Navid
Nguyen, Nam-Trung
RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title_full RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title_fullStr RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title_full_unstemmed RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title_short RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
title_sort rhoa and rac1 in liver cancer cells: induction of overexpression using mechanical stimulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463892/
https://www.ncbi.nlm.nih.gov/pubmed/32731493
http://dx.doi.org/10.3390/mi11080729
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