Cargando…

Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis

The gut microbiota comprises a large and diverse community of bacteria that play a significant role in swine health. Indeed, there is a tight association between the enteric immune system and the overall composition and richness of the microbiota, which is key in the induction, training and function...

Descripción completa

Detalles Bibliográficos
Autores principales: Munyaka, Peris M., Blanc, Fany, Estellé, Jordi, Lemonnier, Gaëtan, Leplat, Jean-Jacques, Rossignol, Marie-Noëlle, Jardet, Déborah, Plastow, Graham, Billon, Yvon, Willing, Benjamin P., Rogel-Gaillard, Claire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464067/
https://www.ncbi.nlm.nih.gov/pubmed/32751315
http://dx.doi.org/10.3390/microorganisms8081151
_version_ 1783577279031410688
author Munyaka, Peris M.
Blanc, Fany
Estellé, Jordi
Lemonnier, Gaëtan
Leplat, Jean-Jacques
Rossignol, Marie-Noëlle
Jardet, Déborah
Plastow, Graham
Billon, Yvon
Willing, Benjamin P.
Rogel-Gaillard, Claire
author_facet Munyaka, Peris M.
Blanc, Fany
Estellé, Jordi
Lemonnier, Gaëtan
Leplat, Jean-Jacques
Rossignol, Marie-Noëlle
Jardet, Déborah
Plastow, Graham
Billon, Yvon
Willing, Benjamin P.
Rogel-Gaillard, Claire
author_sort Munyaka, Peris M.
collection PubMed
description The gut microbiota comprises a large and diverse community of bacteria that play a significant role in swine health. Indeed, there is a tight association between the enteric immune system and the overall composition and richness of the microbiota, which is key in the induction, training and function of the host immunity, and may therefore, influence the immune response to vaccination. Using vaccination against Mycoplasma hyopneumoniae (M. hyo) as a model, we investigated the potential of early-life gut microbiota in predicting vaccine response and explored the post-vaccination dynamics of fecal microbiota at later time points. At 28 days of age (0 days post-vaccination; dpv), healthy piglets were vaccinated, and a booster vaccine was administered at 21 dpv. Blood samples were collected at 0, 21, 28, 35, and 118 dpv to measure M. hyo-specific IgG levels. Fecal samples for 16S rRNA gene amplicon sequencing were collected at 0, 21, 35, and 118 dpv. The results showed variability in antibody response among individual pigs, whilst pre-vaccination operational taxonomic units (OTUs) primarily belonging to Prevotella, [Prevotella], Anaerovibrio, and Sutterella appeared to best-predict vaccine response. Microbiota composition did not differ between the vaccinated and non-vaccinated pigs at post-vaccination time points, but the time effect was significant irrespective of the animals’ vaccination status. Our study provides insight into the role of pre-vaccination gut microbiota composition in vaccine response and emphasizes the importance of studies on full metagenomes and microbial metabolites aimed at deciphering the role of specific bacteria and bacterial genes in the modulation of vaccine response.
format Online
Article
Text
id pubmed-7464067
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74640672020-09-04 Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis Munyaka, Peris M. Blanc, Fany Estellé, Jordi Lemonnier, Gaëtan Leplat, Jean-Jacques Rossignol, Marie-Noëlle Jardet, Déborah Plastow, Graham Billon, Yvon Willing, Benjamin P. Rogel-Gaillard, Claire Microorganisms Article The gut microbiota comprises a large and diverse community of bacteria that play a significant role in swine health. Indeed, there is a tight association between the enteric immune system and the overall composition and richness of the microbiota, which is key in the induction, training and function of the host immunity, and may therefore, influence the immune response to vaccination. Using vaccination against Mycoplasma hyopneumoniae (M. hyo) as a model, we investigated the potential of early-life gut microbiota in predicting vaccine response and explored the post-vaccination dynamics of fecal microbiota at later time points. At 28 days of age (0 days post-vaccination; dpv), healthy piglets were vaccinated, and a booster vaccine was administered at 21 dpv. Blood samples were collected at 0, 21, 28, 35, and 118 dpv to measure M. hyo-specific IgG levels. Fecal samples for 16S rRNA gene amplicon sequencing were collected at 0, 21, 35, and 118 dpv. The results showed variability in antibody response among individual pigs, whilst pre-vaccination operational taxonomic units (OTUs) primarily belonging to Prevotella, [Prevotella], Anaerovibrio, and Sutterella appeared to best-predict vaccine response. Microbiota composition did not differ between the vaccinated and non-vaccinated pigs at post-vaccination time points, but the time effect was significant irrespective of the animals’ vaccination status. Our study provides insight into the role of pre-vaccination gut microbiota composition in vaccine response and emphasizes the importance of studies on full metagenomes and microbial metabolites aimed at deciphering the role of specific bacteria and bacterial genes in the modulation of vaccine response. MDPI 2020-07-29 /pmc/articles/PMC7464067/ /pubmed/32751315 http://dx.doi.org/10.3390/microorganisms8081151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Munyaka, Peris M.
Blanc, Fany
Estellé, Jordi
Lemonnier, Gaëtan
Leplat, Jean-Jacques
Rossignol, Marie-Noëlle
Jardet, Déborah
Plastow, Graham
Billon, Yvon
Willing, Benjamin P.
Rogel-Gaillard, Claire
Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title_full Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title_fullStr Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title_full_unstemmed Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title_short Discovery of Predictors of Mycoplasma hyopneumoniae Vaccine Response Efficiency in Pigs: 16S rRNA Gene Fecal Microbiota Analysis
title_sort discovery of predictors of mycoplasma hyopneumoniae vaccine response efficiency in pigs: 16s rrna gene fecal microbiota analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464067/
https://www.ncbi.nlm.nih.gov/pubmed/32751315
http://dx.doi.org/10.3390/microorganisms8081151
work_keys_str_mv AT munyakaperism discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT blancfany discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT estellejordi discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT lemonniergaetan discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT leplatjeanjacques discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT rossignolmarienoelle discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT jardetdeborah discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT plastowgraham discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT billonyvon discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT willingbenjaminp discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis
AT rogelgaillardclaire discoveryofpredictorsofmycoplasmahyopneumoniaevaccineresponseefficiencyinpigs16srrnagenefecalmicrobiotaanalysis