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Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores

The aim of this study was to report normal measurements of green-emitting fluorophores in the macula of healthy young individuals and to assess the repeatability of these quantitative metrics. To do so, healthy young volunteers were imaged twice (7 ± 3 days apart) using a confocal blue-light fundus...

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Autores principales: Borrelli, Enrico, Battista, Marco, Zuccaro, Biancamaria, Sacconi, Riccardo, Brambati, Maria, Querques, Lea, Prascina, Francesco, Sadda, SriniVas R., Bandello, Francesco, Querques, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464110/
https://www.ncbi.nlm.nih.gov/pubmed/32726903
http://dx.doi.org/10.3390/jcm9082388
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author Borrelli, Enrico
Battista, Marco
Zuccaro, Biancamaria
Sacconi, Riccardo
Brambati, Maria
Querques, Lea
Prascina, Francesco
Sadda, SriniVas R.
Bandello, Francesco
Querques, Giuseppe
author_facet Borrelli, Enrico
Battista, Marco
Zuccaro, Biancamaria
Sacconi, Riccardo
Brambati, Maria
Querques, Lea
Prascina, Francesco
Sadda, SriniVas R.
Bandello, Francesco
Querques, Giuseppe
author_sort Borrelli, Enrico
collection PubMed
description The aim of this study was to report normal measurements of green-emitting fluorophores in the macula of healthy young individuals and to assess the repeatability of these quantitative metrics. To do so, healthy young volunteers were imaged twice (7 ± 3 days apart) using a confocal blue-light fundus autofluorescence (FAF) device with a shorter excitation wavelength (peak at 450 nm) and the capability for separately detecting the red and green components of the emission spectrum. The main outcome measure was the percentage of area occupied by green-emitting fluorophores in the macula. In addition, this measure was performed in separate regions providing a topographical assessment in the foveal, parafoveal and perifoveal regions. Furthermore, the level of agreement between repeated measurements was evaluated. Thirty eyes from 30 healthy volunteers were included in this analysis. Mean age was 26.2 ± 2.8 years (median: 25.0 years; range: 23.0–32.0 years). Median (interquartile range—IQR) area occupied by green-emitting fluorophores was 3.6% (1.9–4.7%) in the macular region. In the topographical analysis, this percentage was higher in the foveal area (median = 33.3%, IQR = 21.9–41.2%), as compared with both the parafoveal (median = 5.3%; IQR = 2.4–8.1%; p < 0.0001) and perifoveal (median = 0.5%, IQR = 0.2–0.8%; p < 0.0001) regions. The coefficient of variation (CV; ranging from 1.1% to 1.7% in the analyzed regions) and the intraclass correlation coefficient (ICC; ranging from 0.93 to 0.97) indicated high levels of repeatability. In conclusion, the assessment of green-emitting fluorophores is repeatable. The distribution of these fluorophores is highest in the foveal region. Assuming that flavin adenine dinucleotide (FAD) emits in the green autofluorescence spectrum, this variability could be secondary to an increased quantity of mitochondria in the foveal region.
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spelling pubmed-74641102020-09-04 Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores Borrelli, Enrico Battista, Marco Zuccaro, Biancamaria Sacconi, Riccardo Brambati, Maria Querques, Lea Prascina, Francesco Sadda, SriniVas R. Bandello, Francesco Querques, Giuseppe J Clin Med Article The aim of this study was to report normal measurements of green-emitting fluorophores in the macula of healthy young individuals and to assess the repeatability of these quantitative metrics. To do so, healthy young volunteers were imaged twice (7 ± 3 days apart) using a confocal blue-light fundus autofluorescence (FAF) device with a shorter excitation wavelength (peak at 450 nm) and the capability for separately detecting the red and green components of the emission spectrum. The main outcome measure was the percentage of area occupied by green-emitting fluorophores in the macula. In addition, this measure was performed in separate regions providing a topographical assessment in the foveal, parafoveal and perifoveal regions. Furthermore, the level of agreement between repeated measurements was evaluated. Thirty eyes from 30 healthy volunteers were included in this analysis. Mean age was 26.2 ± 2.8 years (median: 25.0 years; range: 23.0–32.0 years). Median (interquartile range—IQR) area occupied by green-emitting fluorophores was 3.6% (1.9–4.7%) in the macular region. In the topographical analysis, this percentage was higher in the foveal area (median = 33.3%, IQR = 21.9–41.2%), as compared with both the parafoveal (median = 5.3%; IQR = 2.4–8.1%; p < 0.0001) and perifoveal (median = 0.5%, IQR = 0.2–0.8%; p < 0.0001) regions. The coefficient of variation (CV; ranging from 1.1% to 1.7% in the analyzed regions) and the intraclass correlation coefficient (ICC; ranging from 0.93 to 0.97) indicated high levels of repeatability. In conclusion, the assessment of green-emitting fluorophores is repeatable. The distribution of these fluorophores is highest in the foveal region. Assuming that flavin adenine dinucleotide (FAD) emits in the green autofluorescence spectrum, this variability could be secondary to an increased quantity of mitochondria in the foveal region. MDPI 2020-07-27 /pmc/articles/PMC7464110/ /pubmed/32726903 http://dx.doi.org/10.3390/jcm9082388 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borrelli, Enrico
Battista, Marco
Zuccaro, Biancamaria
Sacconi, Riccardo
Brambati, Maria
Querques, Lea
Prascina, Francesco
Sadda, SriniVas R.
Bandello, Francesco
Querques, Giuseppe
Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title_full Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title_fullStr Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title_full_unstemmed Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title_short Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
title_sort spectrally resolved fundus autofluorescence in healthy eyes: repeatability and topographical analysis of the green-emitting fluorophores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464110/
https://www.ncbi.nlm.nih.gov/pubmed/32726903
http://dx.doi.org/10.3390/jcm9082388
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