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A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation

Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge...

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Autores principales: Hannachi, Nadji, Lepidi, Hubert, Fontanini, Anthony, Takakura, Tatsuki, Bou-Khalil, Jacques, Gouriet, Frédérique, Habib, Gilbert, Raoult, Didier, Camoin-Jau, Laurence, Baudoin, Jean-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464176/
https://www.ncbi.nlm.nih.gov/pubmed/32823780
http://dx.doi.org/10.3390/cells9081899
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author Hannachi, Nadji
Lepidi, Hubert
Fontanini, Anthony
Takakura, Tatsuki
Bou-Khalil, Jacques
Gouriet, Frédérique
Habib, Gilbert
Raoult, Didier
Camoin-Jau, Laurence
Baudoin, Jean-Pierre
author_facet Hannachi, Nadji
Lepidi, Hubert
Fontanini, Anthony
Takakura, Tatsuki
Bou-Khalil, Jacques
Gouriet, Frédérique
Habib, Gilbert
Raoult, Didier
Camoin-Jau, Laurence
Baudoin, Jean-Pierre
author_sort Hannachi, Nadji
collection PubMed
description Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the pathophysiological development and organization of cells in the vegetation, most notably the primordial role of platelets and further triggered hemostasis, which is related to the diversity of infectious microorganisms involved. Our objective was to study the organization of IE vegetations due to different bacteria species in order to understand the related pathophysiological mechanism of vegetation development. We present an approach for ultrastructural analysis of whole-infected heart valve tissue based on scanning electron microscopy and energy-dispersive X-ray spectroscopy. Our approach allowed us to detect differences in cell organization between the analyzed vegetations and revealed a distinct chemical feature in viridans Streptococci ones. Our results illustrate the benefits that such an approach may bring for guiding therapy, considering the germ involved for each IE patient.
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spelling pubmed-74641762020-09-04 A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation Hannachi, Nadji Lepidi, Hubert Fontanini, Anthony Takakura, Tatsuki Bou-Khalil, Jacques Gouriet, Frédérique Habib, Gilbert Raoult, Didier Camoin-Jau, Laurence Baudoin, Jean-Pierre Cells Communication Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the pathophysiological development and organization of cells in the vegetation, most notably the primordial role of platelets and further triggered hemostasis, which is related to the diversity of infectious microorganisms involved. Our objective was to study the organization of IE vegetations due to different bacteria species in order to understand the related pathophysiological mechanism of vegetation development. We present an approach for ultrastructural analysis of whole-infected heart valve tissue based on scanning electron microscopy and energy-dispersive X-ray spectroscopy. Our approach allowed us to detect differences in cell organization between the analyzed vegetations and revealed a distinct chemical feature in viridans Streptococci ones. Our results illustrate the benefits that such an approach may bring for guiding therapy, considering the germ involved for each IE patient. MDPI 2020-08-13 /pmc/articles/PMC7464176/ /pubmed/32823780 http://dx.doi.org/10.3390/cells9081899 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hannachi, Nadji
Lepidi, Hubert
Fontanini, Anthony
Takakura, Tatsuki
Bou-Khalil, Jacques
Gouriet, Frédérique
Habib, Gilbert
Raoult, Didier
Camoin-Jau, Laurence
Baudoin, Jean-Pierre
A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title_full A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title_fullStr A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title_full_unstemmed A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title_short A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
title_sort novel approach for detecting unique variations among infectious bacterial species in endocarditic cardiac valve vegetation
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464176/
https://www.ncbi.nlm.nih.gov/pubmed/32823780
http://dx.doi.org/10.3390/cells9081899
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