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A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation
Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464176/ https://www.ncbi.nlm.nih.gov/pubmed/32823780 http://dx.doi.org/10.3390/cells9081899 |
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author | Hannachi, Nadji Lepidi, Hubert Fontanini, Anthony Takakura, Tatsuki Bou-Khalil, Jacques Gouriet, Frédérique Habib, Gilbert Raoult, Didier Camoin-Jau, Laurence Baudoin, Jean-Pierre |
author_facet | Hannachi, Nadji Lepidi, Hubert Fontanini, Anthony Takakura, Tatsuki Bou-Khalil, Jacques Gouriet, Frédérique Habib, Gilbert Raoult, Didier Camoin-Jau, Laurence Baudoin, Jean-Pierre |
author_sort | Hannachi, Nadji |
collection | PubMed |
description | Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the pathophysiological development and organization of cells in the vegetation, most notably the primordial role of platelets and further triggered hemostasis, which is related to the diversity of infectious microorganisms involved. Our objective was to study the organization of IE vegetations due to different bacteria species in order to understand the related pathophysiological mechanism of vegetation development. We present an approach for ultrastructural analysis of whole-infected heart valve tissue based on scanning electron microscopy and energy-dispersive X-ray spectroscopy. Our approach allowed us to detect differences in cell organization between the analyzed vegetations and revealed a distinct chemical feature in viridans Streptococci ones. Our results illustrate the benefits that such an approach may bring for guiding therapy, considering the germ involved for each IE patient. |
format | Online Article Text |
id | pubmed-7464176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74641762020-09-04 A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation Hannachi, Nadji Lepidi, Hubert Fontanini, Anthony Takakura, Tatsuki Bou-Khalil, Jacques Gouriet, Frédérique Habib, Gilbert Raoult, Didier Camoin-Jau, Laurence Baudoin, Jean-Pierre Cells Communication Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the pathophysiological development and organization of cells in the vegetation, most notably the primordial role of platelets and further triggered hemostasis, which is related to the diversity of infectious microorganisms involved. Our objective was to study the organization of IE vegetations due to different bacteria species in order to understand the related pathophysiological mechanism of vegetation development. We present an approach for ultrastructural analysis of whole-infected heart valve tissue based on scanning electron microscopy and energy-dispersive X-ray spectroscopy. Our approach allowed us to detect differences in cell organization between the analyzed vegetations and revealed a distinct chemical feature in viridans Streptococci ones. Our results illustrate the benefits that such an approach may bring for guiding therapy, considering the germ involved for each IE patient. MDPI 2020-08-13 /pmc/articles/PMC7464176/ /pubmed/32823780 http://dx.doi.org/10.3390/cells9081899 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Hannachi, Nadji Lepidi, Hubert Fontanini, Anthony Takakura, Tatsuki Bou-Khalil, Jacques Gouriet, Frédérique Habib, Gilbert Raoult, Didier Camoin-Jau, Laurence Baudoin, Jean-Pierre A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title | A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title_full | A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title_fullStr | A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title_full_unstemmed | A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title_short | A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation |
title_sort | novel approach for detecting unique variations among infectious bacterial species in endocarditic cardiac valve vegetation |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464176/ https://www.ncbi.nlm.nih.gov/pubmed/32823780 http://dx.doi.org/10.3390/cells9081899 |
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