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Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by excessive lipid deposition. Lipid metabolism disturbances are possibly associated with hepatocyte inflammation development and oxidative balance impairment. The aim of our experiment was to examine the first moment...

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Autores principales: Sztolsztener, Klaudia, Chabowski, Adrian, Harasim-Symbor, Ewa, Bielawiec, Patrycja, Konstantynowicz-Nowicka, Karolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464179/
https://www.ncbi.nlm.nih.gov/pubmed/32751983
http://dx.doi.org/10.3390/biom10081133
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author Sztolsztener, Klaudia
Chabowski, Adrian
Harasim-Symbor, Ewa
Bielawiec, Patrycja
Konstantynowicz-Nowicka, Karolina
author_facet Sztolsztener, Klaudia
Chabowski, Adrian
Harasim-Symbor, Ewa
Bielawiec, Patrycja
Konstantynowicz-Nowicka, Karolina
author_sort Sztolsztener, Klaudia
collection PubMed
description Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by excessive lipid deposition. Lipid metabolism disturbances are possibly associated with hepatocyte inflammation development and oxidative balance impairment. The aim of our experiment was to examine the first moment when changes in plasma and liver arachidonic acid (AA) levels as a pro-inflammatory precursor may occur during high-fat diet (HFD)-induced NAFLD development. Wistar rats were fed a diet rich in fat for five weeks, and after each week, inflammation and redox balance parameters were evaluated in the liver. The AA contents in lipid fractions were assessed by gas–liquid chromatography (GLC). Protein expression relevant to inflammatory and lipogenesis pathways was determined by immunoblotting. The oxidative system indicators were determined with assay kits. Our results revealed that a high-fat diet promoted an increase in AA levels, especially in the phospholipid (PL) fraction. Importantly, rapid inflammation development via increased inflammatory enzyme expression, elevated lipid peroxidation product content and oxidative system impairment was caused by the HFD as early as the first week of the experiment. Based on these results, we may postulate that changes in AA content may be an early indicator of inflammation and irreversible changes in NAFLD progression.
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spelling pubmed-74641792020-09-04 Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development Sztolsztener, Klaudia Chabowski, Adrian Harasim-Symbor, Ewa Bielawiec, Patrycja Konstantynowicz-Nowicka, Karolina Biomolecules Article Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease characterized by excessive lipid deposition. Lipid metabolism disturbances are possibly associated with hepatocyte inflammation development and oxidative balance impairment. The aim of our experiment was to examine the first moment when changes in plasma and liver arachidonic acid (AA) levels as a pro-inflammatory precursor may occur during high-fat diet (HFD)-induced NAFLD development. Wistar rats were fed a diet rich in fat for five weeks, and after each week, inflammation and redox balance parameters were evaluated in the liver. The AA contents in lipid fractions were assessed by gas–liquid chromatography (GLC). Protein expression relevant to inflammatory and lipogenesis pathways was determined by immunoblotting. The oxidative system indicators were determined with assay kits. Our results revealed that a high-fat diet promoted an increase in AA levels, especially in the phospholipid (PL) fraction. Importantly, rapid inflammation development via increased inflammatory enzyme expression, elevated lipid peroxidation product content and oxidative system impairment was caused by the HFD as early as the first week of the experiment. Based on these results, we may postulate that changes in AA content may be an early indicator of inflammation and irreversible changes in NAFLD progression. MDPI 2020-07-31 /pmc/articles/PMC7464179/ /pubmed/32751983 http://dx.doi.org/10.3390/biom10081133 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sztolsztener, Klaudia
Chabowski, Adrian
Harasim-Symbor, Ewa
Bielawiec, Patrycja
Konstantynowicz-Nowicka, Karolina
Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title_full Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title_fullStr Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title_full_unstemmed Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title_short Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development
title_sort arachidonic acid as an early indicator of inflammation during non-alcoholic fatty liver disease development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464179/
https://www.ncbi.nlm.nih.gov/pubmed/32751983
http://dx.doi.org/10.3390/biom10081133
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