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Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin

Background: Pinopode expression has been suggested as a marker of endometrial receptivity. Methods: We set up an experimental study comparing endometrial tissue from recurrent pregnancy loss (RPL, n = 30) and fertile control (CTR, n = 20) women in terms of pinopode expression/morphology; expression...

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Autores principales: D’Ippolito, Silvia, Di Nicuolo, Fiorella, Papi, Massimiliano, Castellani, Roberta, Palmieri, Valentina, Masciullo, Valeria, Arena, Vincenzo, Tersigni, Chiara, Bernabei, Micaela, Pontecorvi, Alfredo, Scambia, Giovanni, Di Simone, Nicoletta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464296/
https://www.ncbi.nlm.nih.gov/pubmed/32823767
http://dx.doi.org/10.3390/jcm9082634
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author D’Ippolito, Silvia
Di Nicuolo, Fiorella
Papi, Massimiliano
Castellani, Roberta
Palmieri, Valentina
Masciullo, Valeria
Arena, Vincenzo
Tersigni, Chiara
Bernabei, Micaela
Pontecorvi, Alfredo
Scambia, Giovanni
Di Simone, Nicoletta
author_facet D’Ippolito, Silvia
Di Nicuolo, Fiorella
Papi, Massimiliano
Castellani, Roberta
Palmieri, Valentina
Masciullo, Valeria
Arena, Vincenzo
Tersigni, Chiara
Bernabei, Micaela
Pontecorvi, Alfredo
Scambia, Giovanni
Di Simone, Nicoletta
author_sort D’Ippolito, Silvia
collection PubMed
description Background: Pinopode expression has been suggested as a marker of endometrial receptivity. Methods: We set up an experimental study comparing endometrial tissue from recurrent pregnancy loss (RPL, n = 30) and fertile control (CTR, n = 20) women in terms of pinopode expression/morphology; expression of thrombomodulin (TM) and ezrin; cytoskeletal organization. Endometrial samples were collected during implantation window and evaluated by scanning electron microscopy, western blot, and immunofluorescence. Results: We found that RPL endometrial tissue showed: (i) increased pinopodes density (* p < 0.05); (ii) a reduced diameter of pinopodes (* p < 0.05); (iii) a decreased TM and ezrin expression (p < 0.05). Additionally, confocal images showed a significantly reduced expression of phosphorylated (p)-ezrin, confirming the results obtained through immunoblot analysis. Immunofluorescence staining showed that in CTR samples, junctions between cells are intact and clearly visible, whereas actin filaments appear completely lost in RPL endometrial samples; this suggests that, due to the impaired expression and activity of TM and ezrin, actin does not bind to plasma membrane in order to orchestrate the cytoskeletal actin filaments. Conclusions: Our findings suggest that an impaired expression of TM and expression/activation of ezrin may affect the connection between the TM and actin cytoskeleton, impairing the organization of cytoskeleton and, eventually, the adequate pinopode development.
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spelling pubmed-74642962020-09-04 Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin D’Ippolito, Silvia Di Nicuolo, Fiorella Papi, Massimiliano Castellani, Roberta Palmieri, Valentina Masciullo, Valeria Arena, Vincenzo Tersigni, Chiara Bernabei, Micaela Pontecorvi, Alfredo Scambia, Giovanni Di Simone, Nicoletta J Clin Med Article Background: Pinopode expression has been suggested as a marker of endometrial receptivity. Methods: We set up an experimental study comparing endometrial tissue from recurrent pregnancy loss (RPL, n = 30) and fertile control (CTR, n = 20) women in terms of pinopode expression/morphology; expression of thrombomodulin (TM) and ezrin; cytoskeletal organization. Endometrial samples were collected during implantation window and evaluated by scanning electron microscopy, western blot, and immunofluorescence. Results: We found that RPL endometrial tissue showed: (i) increased pinopodes density (* p < 0.05); (ii) a reduced diameter of pinopodes (* p < 0.05); (iii) a decreased TM and ezrin expression (p < 0.05). Additionally, confocal images showed a significantly reduced expression of phosphorylated (p)-ezrin, confirming the results obtained through immunoblot analysis. Immunofluorescence staining showed that in CTR samples, junctions between cells are intact and clearly visible, whereas actin filaments appear completely lost in RPL endometrial samples; this suggests that, due to the impaired expression and activity of TM and ezrin, actin does not bind to plasma membrane in order to orchestrate the cytoskeletal actin filaments. Conclusions: Our findings suggest that an impaired expression of TM and expression/activation of ezrin may affect the connection between the TM and actin cytoskeleton, impairing the organization of cytoskeleton and, eventually, the adequate pinopode development. MDPI 2020-08-13 /pmc/articles/PMC7464296/ /pubmed/32823767 http://dx.doi.org/10.3390/jcm9082634 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Ippolito, Silvia
Di Nicuolo, Fiorella
Papi, Massimiliano
Castellani, Roberta
Palmieri, Valentina
Masciullo, Valeria
Arena, Vincenzo
Tersigni, Chiara
Bernabei, Micaela
Pontecorvi, Alfredo
Scambia, Giovanni
Di Simone, Nicoletta
Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title_full Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title_fullStr Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title_full_unstemmed Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title_short Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin
title_sort expression of pinopodes in the endometrium from recurrent pregnancy loss women. role of thrombomodulin and ezrin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464296/
https://www.ncbi.nlm.nih.gov/pubmed/32823767
http://dx.doi.org/10.3390/jcm9082634
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