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Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms

Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to fi...

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Autores principales: Soumoy, Laura, Wells, Mathilde, Najem, Ahmad, Krayem, Mohammad, Ghanem, Ghanem, Hambye, Stéphanie, Saussez, Sven, Blankert, Bertrand, Journe, Fabrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464305/
https://www.ncbi.nlm.nih.gov/pubmed/32785105
http://dx.doi.org/10.3390/biology9080218
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author Soumoy, Laura
Wells, Mathilde
Najem, Ahmad
Krayem, Mohammad
Ghanem, Ghanem
Hambye, Stéphanie
Saussez, Sven
Blankert, Bertrand
Journe, Fabrice
author_facet Soumoy, Laura
Wells, Mathilde
Najem, Ahmad
Krayem, Mohammad
Ghanem, Ghanem
Hambye, Stéphanie
Saussez, Sven
Blankert, Bertrand
Journe, Fabrice
author_sort Soumoy, Laura
collection PubMed
description Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to find new therapeutic agents to increase patient survival. Toad venoms are a rich source of potentially pharmaceutically active compounds and studies have highlighted their possible effect on cancer cells. We focused on the venoms of two different toad species: Bufo bufo and Rhinella marina. We screened the venom crude extracts, the fractions from crude extracts and isolated biomolecules by studying their antiproliferative properties on melanoma cells aiming to determine the compound or the combination of compounds with the highest antiproliferative effect. Our results indicated strong antiproliferative capacities of toad venoms on melanoma cells. We found that these effects were mainly due to bufadienolides that are cardiotonic steroids potentially acting on the Na(+)/K(+) ATPase pump which is overexpressed in melanoma. Finally, our results indicated that bufalin alone was the most interesting compound among the isolated bufadienolides because it had the highest antiproliferative activity on melanoma cells.
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spelling pubmed-74643052020-09-04 Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms Soumoy, Laura Wells, Mathilde Najem, Ahmad Krayem, Mohammad Ghanem, Ghanem Hambye, Stéphanie Saussez, Sven Blankert, Bertrand Journe, Fabrice Biology (Basel) Article Melanoma is the most common cancer in young adults, with a constantly increasing incidence. Metastatic melanoma is a very aggressive cancer with a 5-year survival rate of about 22−25%. This is, in most cases, due to a lack of therapies which are effective on the long term. Hence, it is crucial to find new therapeutic agents to increase patient survival. Toad venoms are a rich source of potentially pharmaceutically active compounds and studies have highlighted their possible effect on cancer cells. We focused on the venoms of two different toad species: Bufo bufo and Rhinella marina. We screened the venom crude extracts, the fractions from crude extracts and isolated biomolecules by studying their antiproliferative properties on melanoma cells aiming to determine the compound or the combination of compounds with the highest antiproliferative effect. Our results indicated strong antiproliferative capacities of toad venoms on melanoma cells. We found that these effects were mainly due to bufadienolides that are cardiotonic steroids potentially acting on the Na(+)/K(+) ATPase pump which is overexpressed in melanoma. Finally, our results indicated that bufalin alone was the most interesting compound among the isolated bufadienolides because it had the highest antiproliferative activity on melanoma cells. MDPI 2020-08-10 /pmc/articles/PMC7464305/ /pubmed/32785105 http://dx.doi.org/10.3390/biology9080218 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soumoy, Laura
Wells, Mathilde
Najem, Ahmad
Krayem, Mohammad
Ghanem, Ghanem
Hambye, Stéphanie
Saussez, Sven
Blankert, Bertrand
Journe, Fabrice
Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title_full Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title_fullStr Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title_full_unstemmed Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title_short Toad Venom Antiproliferative Activities on Metastatic Melanoma: Bio-Guided Fractionation and Screening of the Compounds of Two Different Venoms
title_sort toad venom antiproliferative activities on metastatic melanoma: bio-guided fractionation and screening of the compounds of two different venoms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464305/
https://www.ncbi.nlm.nih.gov/pubmed/32785105
http://dx.doi.org/10.3390/biology9080218
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