Cargando…

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models

There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Mantecón-Oria, Marián, Diban, Nazely, Berciano, Maria T., Rivero, Maria J., David, Oana, Lafarga, Miguel, Tapia, Olga, Urtiaga, Ane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464335/
https://www.ncbi.nlm.nih.gov/pubmed/32708027
http://dx.doi.org/10.3390/membranes10080161
_version_ 1783577341007495168
author Mantecón-Oria, Marián
Diban, Nazely
Berciano, Maria T.
Rivero, Maria J.
David, Oana
Lafarga, Miguel
Tapia, Olga
Urtiaga, Ane
author_facet Mantecón-Oria, Marián
Diban, Nazely
Berciano, Maria T.
Rivero, Maria J.
David, Oana
Lafarga, Miguel
Tapia, Olga
Urtiaga, Ane
author_sort Mantecón-Oria, Marián
collection PubMed
description There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.
format Online
Article
Text
id pubmed-7464335
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74643352020-09-04 Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models Mantecón-Oria, Marián Diban, Nazely Berciano, Maria T. Rivero, Maria J. David, Oana Lafarga, Miguel Tapia, Olga Urtiaga, Ane Membranes (Basel) Article There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems. MDPI 2020-07-22 /pmc/articles/PMC7464335/ /pubmed/32708027 http://dx.doi.org/10.3390/membranes10080161 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mantecón-Oria, Marián
Diban, Nazely
Berciano, Maria T.
Rivero, Maria J.
David, Oana
Lafarga, Miguel
Tapia, Olga
Urtiaga, Ane
Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title_full Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title_fullStr Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title_full_unstemmed Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title_short Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models
title_sort hollow fiber membranes of pcl and pcl/graphene as scaffolds with potential to develop in vitro blood—brain barrier models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464335/
https://www.ncbi.nlm.nih.gov/pubmed/32708027
http://dx.doi.org/10.3390/membranes10080161
work_keys_str_mv AT manteconoriamarian hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT dibannazely hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT bercianomariat hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT riveromariaj hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT davidoana hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT lafargamiguel hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT tapiaolga hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels
AT urtiagaane hollowfibermembranesofpclandpclgrapheneasscaffoldswithpotentialtodevelopinvitrobloodbrainbarriermodels