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Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines

This current study investigates the facilitative effects and mechanisms of decursin, a major component of Angelica gigas Nakai (AGN), and AGN root extract on hair growth in mice. We perform high-performance liquid chromatography on AGN extract to show it contains 7.3% decursin. Hairs in mouse dorsal...

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Autores principales: Lee, Tae-Kyeong, Kim, Bora, Kim, Dae Won, Ahn, Ji Hyeon, Sim, Hyejin, Lee, Jae-Chul, Yang, Go Eun, Her, Young, Park, Joon Ha, Kim, Hyun Sook, Sim, Tae Heung, Lee, Hyun Sam, Won, Moo-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464339/
https://www.ncbi.nlm.nih.gov/pubmed/32823713
http://dx.doi.org/10.3390/molecules25163697
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author Lee, Tae-Kyeong
Kim, Bora
Kim, Dae Won
Ahn, Ji Hyeon
Sim, Hyejin
Lee, Jae-Chul
Yang, Go Eun
Her, Young
Park, Joon Ha
Kim, Hyun Sook
Sim, Tae Heung
Lee, Hyun Sam
Won, Moo-Ho
author_facet Lee, Tae-Kyeong
Kim, Bora
Kim, Dae Won
Ahn, Ji Hyeon
Sim, Hyejin
Lee, Jae-Chul
Yang, Go Eun
Her, Young
Park, Joon Ha
Kim, Hyun Sook
Sim, Tae Heung
Lee, Hyun Sam
Won, Moo-Ho
author_sort Lee, Tae-Kyeong
collection PubMed
description This current study investigates the facilitative effects and mechanisms of decursin, a major component of Angelica gigas Nakai (AGN), and AGN root extract on hair growth in mice. We perform high-performance liquid chromatography on AGN extract to show it contains 7.3% decursin. Hairs in mouse dorsal skin are shaved distilled in water, 0.15% decursin, and 2% AGN root extract (0.15% decursin in the diluted extract) and topically applied twice a day for 17 days. Hematoxylin and eosin staining are done to examine the morphological changes in the hair follicles. To compare the effects of decursin and AGN extract on inflammatory cytokines in the dorsal skin, Western blot analysis and immunohistochemistry for tumor necrosis factor α (TNF-α) and interleukin (IL)-1β as pro-inflammatory cytokines, and IL-4 and IL-13 as anti-inflammatory cytokines are conducted. The results show that the application of decursin and AGN extract confer effects on hair growth. Hair growth is significantly facilitated from seven days after the treatments compared to that in the control group, and completely grown hair was found 17 days after the treatments. The protein levels and immunoreactivity of TNF-α and IL-1β in this case are significantly decreased, whereas the IL-4 and IL-13 levels and immunoreactivity are significantly increased compared to those in the control group. Additionally, high-mobility group box 1, an inflammatory mediator, is elevated by the topical application of decursin and AGN extract. Taken together, the treatment of mouse dorsal skin with AGE root extract containing decursin promotes hair growth by regulating pro- and/or anti-inflammatory cytokines. We, therefore, suggest that AGN root extract as well as decursin can be utilized as materials for developing hair growth-facilitating treatments.
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spelling pubmed-74643392020-09-04 Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines Lee, Tae-Kyeong Kim, Bora Kim, Dae Won Ahn, Ji Hyeon Sim, Hyejin Lee, Jae-Chul Yang, Go Eun Her, Young Park, Joon Ha Kim, Hyun Sook Sim, Tae Heung Lee, Hyun Sam Won, Moo-Ho Molecules Article This current study investigates the facilitative effects and mechanisms of decursin, a major component of Angelica gigas Nakai (AGN), and AGN root extract on hair growth in mice. We perform high-performance liquid chromatography on AGN extract to show it contains 7.3% decursin. Hairs in mouse dorsal skin are shaved distilled in water, 0.15% decursin, and 2% AGN root extract (0.15% decursin in the diluted extract) and topically applied twice a day for 17 days. Hematoxylin and eosin staining are done to examine the morphological changes in the hair follicles. To compare the effects of decursin and AGN extract on inflammatory cytokines in the dorsal skin, Western blot analysis and immunohistochemistry for tumor necrosis factor α (TNF-α) and interleukin (IL)-1β as pro-inflammatory cytokines, and IL-4 and IL-13 as anti-inflammatory cytokines are conducted. The results show that the application of decursin and AGN extract confer effects on hair growth. Hair growth is significantly facilitated from seven days after the treatments compared to that in the control group, and completely grown hair was found 17 days after the treatments. The protein levels and immunoreactivity of TNF-α and IL-1β in this case are significantly decreased, whereas the IL-4 and IL-13 levels and immunoreactivity are significantly increased compared to those in the control group. Additionally, high-mobility group box 1, an inflammatory mediator, is elevated by the topical application of decursin and AGN extract. Taken together, the treatment of mouse dorsal skin with AGE root extract containing decursin promotes hair growth by regulating pro- and/or anti-inflammatory cytokines. We, therefore, suggest that AGN root extract as well as decursin can be utilized as materials for developing hair growth-facilitating treatments. MDPI 2020-08-13 /pmc/articles/PMC7464339/ /pubmed/32823713 http://dx.doi.org/10.3390/molecules25163697 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Tae-Kyeong
Kim, Bora
Kim, Dae Won
Ahn, Ji Hyeon
Sim, Hyejin
Lee, Jae-Chul
Yang, Go Eun
Her, Young
Park, Joon Ha
Kim, Hyun Sook
Sim, Tae Heung
Lee, Hyun Sam
Won, Moo-Ho
Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title_full Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title_fullStr Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title_full_unstemmed Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title_short Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines
title_sort effects of decursin and angelica gigas nakai root extract on hair growth in mouse dorsal skin via regulating inflammatory cytokines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464339/
https://www.ncbi.nlm.nih.gov/pubmed/32823713
http://dx.doi.org/10.3390/molecules25163697
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