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Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina

In microalgae, photosynthesis provides energy and sugar phosphates for the biosynthesis of storage and structural carbohydrates, lipids, and nitrogenous proteins. The oleaginous alga Nannochloropsis salina does not preferentially partition photoassimilates among cellulose, chrysolaminarin, and lipid...

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Autores principales: Jeong, Seok Won, HwangBo, Kwon, Lim, Jong Min, Nam, Seung Won, Lee, Bong Soo, Jeong, Byeong-ryool, Chang, Yong Keun, Jeong, Won-Joong, Park, Youn-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464416/
https://www.ncbi.nlm.nih.gov/pubmed/32781613
http://dx.doi.org/10.3390/microorganisms8081195
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author Jeong, Seok Won
HwangBo, Kwon
Lim, Jong Min
Nam, Seung Won
Lee, Bong Soo
Jeong, Byeong-ryool
Chang, Yong Keun
Jeong, Won-Joong
Park, Youn-Il
author_facet Jeong, Seok Won
HwangBo, Kwon
Lim, Jong Min
Nam, Seung Won
Lee, Bong Soo
Jeong, Byeong-ryool
Chang, Yong Keun
Jeong, Won-Joong
Park, Youn-Il
author_sort Jeong, Seok Won
collection PubMed
description In microalgae, photosynthesis provides energy and sugar phosphates for the biosynthesis of storage and structural carbohydrates, lipids, and nitrogenous proteins. The oleaginous alga Nannochloropsis salina does not preferentially partition photoassimilates among cellulose, chrysolaminarin, and lipids in response to nitrogenous nutrient deprivation. In the present study, we investigated whether genetic impairment of the cellulose synthase gene (CesA) expression would lead to protein accumulation without the accumulation of storage C polymers in N. salina. Three cesA mutants were generated by the CRISPR/Cas9 approach. Cell wall thickness and cellulose content were reduced in the cesA1 mutant, but not in cesA2 or cesA4 cells. CesA1 mutation resulted in a reduction of chrysolaminarin and neutral lipid contents, by 66.3% and 37.1%, respectively, but increased the soluble protein content by 1.8-fold. Further, N. salina cells with a thinned cell wall were susceptible to mechanical stress, resulting in a 1.7-fold enhancement of lipid extractability. Taken together, the previous and current studies strongly suggest the presence of a controlling mechanism that regulates photoassimilate partitioning toward C and N metabolic pathways as well as the cellulose metabolism as a potential target for cost-effective microalgal cell disruption and as a useful protein production platform.
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spelling pubmed-74644162020-09-04 Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina Jeong, Seok Won HwangBo, Kwon Lim, Jong Min Nam, Seung Won Lee, Bong Soo Jeong, Byeong-ryool Chang, Yong Keun Jeong, Won-Joong Park, Youn-Il Microorganisms Article In microalgae, photosynthesis provides energy and sugar phosphates for the biosynthesis of storage and structural carbohydrates, lipids, and nitrogenous proteins. The oleaginous alga Nannochloropsis salina does not preferentially partition photoassimilates among cellulose, chrysolaminarin, and lipids in response to nitrogenous nutrient deprivation. In the present study, we investigated whether genetic impairment of the cellulose synthase gene (CesA) expression would lead to protein accumulation without the accumulation of storage C polymers in N. salina. Three cesA mutants were generated by the CRISPR/Cas9 approach. Cell wall thickness and cellulose content were reduced in the cesA1 mutant, but not in cesA2 or cesA4 cells. CesA1 mutation resulted in a reduction of chrysolaminarin and neutral lipid contents, by 66.3% and 37.1%, respectively, but increased the soluble protein content by 1.8-fold. Further, N. salina cells with a thinned cell wall were susceptible to mechanical stress, resulting in a 1.7-fold enhancement of lipid extractability. Taken together, the previous and current studies strongly suggest the presence of a controlling mechanism that regulates photoassimilate partitioning toward C and N metabolic pathways as well as the cellulose metabolism as a potential target for cost-effective microalgal cell disruption and as a useful protein production platform. MDPI 2020-08-06 /pmc/articles/PMC7464416/ /pubmed/32781613 http://dx.doi.org/10.3390/microorganisms8081195 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jeong, Seok Won
HwangBo, Kwon
Lim, Jong Min
Nam, Seung Won
Lee, Bong Soo
Jeong, Byeong-ryool
Chang, Yong Keun
Jeong, Won-Joong
Park, Youn-Il
Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title_full Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title_fullStr Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title_full_unstemmed Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title_short Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina
title_sort genetic impairment of cellulose biosynthesis increases cell wall fragility and improves lipid extractability from oleaginous alga nannochloropsis salina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464416/
https://www.ncbi.nlm.nih.gov/pubmed/32781613
http://dx.doi.org/10.3390/microorganisms8081195
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