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Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs
The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464425/ https://www.ncbi.nlm.nih.gov/pubmed/32784762 http://dx.doi.org/10.3390/metabo10080322 |
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author | Taniguchi, Masaaki Arakawa, Aisaku Nishio, Motohide Okamura, Toshihiro Ohnishi, Chika Kadowaki, Kouen Kohira, Kimiko Homma, Fumika Matsumoto, Kazunori Ishii, Kazuo |
author_facet | Taniguchi, Masaaki Arakawa, Aisaku Nishio, Motohide Okamura, Toshihiro Ohnishi, Chika Kadowaki, Kouen Kohira, Kimiko Homma, Fumika Matsumoto, Kazunori Ishii, Kazuo |
author_sort | Taniguchi, Masaaki |
collection | PubMed |
description | The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however, no studies have investigated their use during the fattening period of pigs. This study examined the metabolite profiles in the plasma of two groups of pigs derived from the same Duroc genetic line and fed the same diet. Five plasma samples were collected from each individual the day before slaughter. Capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS) was used to analyze the purified plasma from each sample. Principle component analysis (PCA) and partial least squares (PLS) were used to find the semi-quantitative values of the compounds. The results indicate that branched-chain amino acids are significantly associated with high IMF content, while amino acids are associated with low IMF content. These differences were validated using the quantification analyses by high-performance liquid chromatograph, which supported our results. These results suggest that the concentration of branched-chain amino acids in plasma could be an indicative biomarker for the IMF content in the loin eye area. |
format | Online Article Text |
id | pubmed-7464425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74644252020-09-04 Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs Taniguchi, Masaaki Arakawa, Aisaku Nishio, Motohide Okamura, Toshihiro Ohnishi, Chika Kadowaki, Kouen Kohira, Kimiko Homma, Fumika Matsumoto, Kazunori Ishii, Kazuo Metabolites Article The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however, no studies have investigated their use during the fattening period of pigs. This study examined the metabolite profiles in the plasma of two groups of pigs derived from the same Duroc genetic line and fed the same diet. Five plasma samples were collected from each individual the day before slaughter. Capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS) was used to analyze the purified plasma from each sample. Principle component analysis (PCA) and partial least squares (PLS) were used to find the semi-quantitative values of the compounds. The results indicate that branched-chain amino acids are significantly associated with high IMF content, while amino acids are associated with low IMF content. These differences were validated using the quantification analyses by high-performance liquid chromatograph, which supported our results. These results suggest that the concentration of branched-chain amino acids in plasma could be an indicative biomarker for the IMF content in the loin eye area. MDPI 2020-08-07 /pmc/articles/PMC7464425/ /pubmed/32784762 http://dx.doi.org/10.3390/metabo10080322 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Taniguchi, Masaaki Arakawa, Aisaku Nishio, Motohide Okamura, Toshihiro Ohnishi, Chika Kadowaki, Kouen Kohira, Kimiko Homma, Fumika Matsumoto, Kazunori Ishii, Kazuo Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title | Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title_full | Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title_fullStr | Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title_full_unstemmed | Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title_short | Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs |
title_sort | differential metabolomics profiles identified by ce-tofms between high and low intramuscular fat amount in fattening pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464425/ https://www.ncbi.nlm.nih.gov/pubmed/32784762 http://dx.doi.org/10.3390/metabo10080322 |
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