Cargando…

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Plant-specific NAC (NAM, ATAF, CUC) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in Cleistogenes songorica, a prominent xerophyte desert grass in northwestern China. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Zong, Xifang, Yan, Qi, Wu, Fan, Ma, Qian, Zhang, Jiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464430/
https://www.ncbi.nlm.nih.gov/pubmed/32806602
http://dx.doi.org/10.3390/genes11080927
_version_ 1783577363321192448
author Zong, Xifang
Yan, Qi
Wu, Fan
Ma, Qian
Zhang, Jiyu
author_facet Zong, Xifang
Yan, Qi
Wu, Fan
Ma, Qian
Zhang, Jiyu
author_sort Zong, Xifang
collection PubMed
description Plant-specific NAC (NAM, ATAF, CUC) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in Cleistogenes songorica, a prominent xerophyte desert grass in northwestern China. In this study, 162 NAC genes were found from the Cleistogenes songorica genome, among which 156 C. songorica NAC (CsNAC) genes (96.3%) were mapped onto 20 chromosomes. The phylogenetic tree constructed by CsNAC and rice NAC TFs can be separated into 14 subfamilies. Syntenic and Ka/Ks analyses showed that CsNACs were primarily expanded by genomewide replication events, and purifying selection was the primary force driving the evolution of CsNAC family genes. The CsNAC gene expression profiles showed that 36 CsNAC genes showed differential expression between cleistogamous (CL) and chasmogamous (CH) flowers. One hundred and two CsNAC genes showed differential expression under heat, cold, drought, salt and ABA treatment. Twenty-three CsNAC genes were commonly differentially expressed both under stress responses and during dimorphic floret development. Gene Ontology (GO) annotation, coexpression network and qRT-PCR tests revealed that these CsNAC genes may simultaneously regulate dimorphic floret development and the response to stress. Our results may help to characterize the NAC transcription factors in C. songorica and provide new insights into the functional research and application of the NAC family in crop improvement, especially in dimorphic floret plants.
format Online
Article
Text
id pubmed-7464430
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74644302020-09-04 Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica Zong, Xifang Yan, Qi Wu, Fan Ma, Qian Zhang, Jiyu Genes (Basel) Article Plant-specific NAC (NAM, ATAF, CUC) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in Cleistogenes songorica, a prominent xerophyte desert grass in northwestern China. In this study, 162 NAC genes were found from the Cleistogenes songorica genome, among which 156 C. songorica NAC (CsNAC) genes (96.3%) were mapped onto 20 chromosomes. The phylogenetic tree constructed by CsNAC and rice NAC TFs can be separated into 14 subfamilies. Syntenic and Ka/Ks analyses showed that CsNACs were primarily expanded by genomewide replication events, and purifying selection was the primary force driving the evolution of CsNAC family genes. The CsNAC gene expression profiles showed that 36 CsNAC genes showed differential expression between cleistogamous (CL) and chasmogamous (CH) flowers. One hundred and two CsNAC genes showed differential expression under heat, cold, drought, salt and ABA treatment. Twenty-three CsNAC genes were commonly differentially expressed both under stress responses and during dimorphic floret development. Gene Ontology (GO) annotation, coexpression network and qRT-PCR tests revealed that these CsNAC genes may simultaneously regulate dimorphic floret development and the response to stress. Our results may help to characterize the NAC transcription factors in C. songorica and provide new insights into the functional research and application of the NAC family in crop improvement, especially in dimorphic floret plants. MDPI 2020-08-12 /pmc/articles/PMC7464430/ /pubmed/32806602 http://dx.doi.org/10.3390/genes11080927 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zong, Xifang
Yan, Qi
Wu, Fan
Ma, Qian
Zhang, Jiyu
Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title_full Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title_fullStr Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title_full_unstemmed Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title_short Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica
title_sort genome-wide analysis of the role of nac family in flower development and abiotic stress responses in cleistogenes songorica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464430/
https://www.ncbi.nlm.nih.gov/pubmed/32806602
http://dx.doi.org/10.3390/genes11080927
work_keys_str_mv AT zongxifang genomewideanalysisoftheroleofnacfamilyinflowerdevelopmentandabioticstressresponsesincleistogenessongorica
AT yanqi genomewideanalysisoftheroleofnacfamilyinflowerdevelopmentandabioticstressresponsesincleistogenessongorica
AT wufan genomewideanalysisoftheroleofnacfamilyinflowerdevelopmentandabioticstressresponsesincleistogenessongorica
AT maqian genomewideanalysisoftheroleofnacfamilyinflowerdevelopmentandabioticstressresponsesincleistogenessongorica
AT zhangjiyu genomewideanalysisoftheroleofnacfamilyinflowerdevelopmentandabioticstressresponsesincleistogenessongorica