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Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China
Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464434/ https://www.ncbi.nlm.nih.gov/pubmed/32784367 http://dx.doi.org/10.3390/molecules25163605 |
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author | Jin, Haijun Yu, Hua Wang, Haixia Zhang, Jia |
author_facet | Jin, Haijun Yu, Hua Wang, Haixia Zhang, Jia |
author_sort | Jin, Haijun |
collection | PubMed |
description | Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality. |
format | Online Article Text |
id | pubmed-7464434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74644342020-09-04 Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China Jin, Haijun Yu, Hua Wang, Haixia Zhang, Jia Molecules Article Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality. MDPI 2020-08-08 /pmc/articles/PMC7464434/ /pubmed/32784367 http://dx.doi.org/10.3390/molecules25163605 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jin, Haijun Yu, Hua Wang, Haixia Zhang, Jia Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title | Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title_full | Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title_fullStr | Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title_full_unstemmed | Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title_short | Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China |
title_sort | comparative proteomic analysis of dipsacus asperoides roots from different habitats in china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464434/ https://www.ncbi.nlm.nih.gov/pubmed/32784367 http://dx.doi.org/10.3390/molecules25163605 |
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