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Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers

Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV...

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Autores principales: Reid, Paul, Staudacher, Alexander H., Marcu, Loredana G., Olver, Ian, Moghaddasi, Leyla, Brown, Michael P., Li, Yanrui, Bezak, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464531/
https://www.ncbi.nlm.nih.gov/pubmed/32727072
http://dx.doi.org/10.3390/cells9081788
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author Reid, Paul
Staudacher, Alexander H.
Marcu, Loredana G.
Olver, Ian
Moghaddasi, Leyla
Brown, Michael P.
Li, Yanrui
Bezak, Eva
author_facet Reid, Paul
Staudacher, Alexander H.
Marcu, Loredana G.
Olver, Ian
Moghaddasi, Leyla
Brown, Michael P.
Li, Yanrui
Bezak, Eva
author_sort Reid, Paul
collection PubMed
description Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV status, in their response to radiation dose. Previously unexamined changes in radio-responsiveness following fractionated X-ray irradiation were compared between HPV positive and negative statuses of HNSCC. Six HNSCC cell lines, 3 of each HPV status, were investigated for radiosensitivity by clonogenic assay and modelled by response as a function of dose. Generational cultures of each cell line were developed to follow changes in radiosensitivity after repeated irradiations simulating fractionated radiation therapy. As a group, the HPV positive cell lines were more radiosensitive, but with changes following repeated fractions of dose, and modelling of response as a function of dose, both statuses displayed large radiobiological heterogeneity. These findings challenge current radiobiological assumptions of head and neck cancers as early responding tissue to radiation and may go some way in explaining difficulties reaching consensus in stratification of treatment by HPV status. Consequently, results from this study do not support stratifying radiation therapy by HPV status.
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spelling pubmed-74645312020-09-04 Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers Reid, Paul Staudacher, Alexander H. Marcu, Loredana G. Olver, Ian Moghaddasi, Leyla Brown, Michael P. Li, Yanrui Bezak, Eva Cells Article Head and neck squamous cell carcinomas (HNSCC) resulting from human papillomavirus (HPV) are increasing in incidence but demonstrate significantly better treatment response than HNSCC from other causes such as tobacco and alcohol. This study sought to identify differences in HNSCC, intrinsic to HPV status, in their response to radiation dose. Previously unexamined changes in radio-responsiveness following fractionated X-ray irradiation were compared between HPV positive and negative statuses of HNSCC. Six HNSCC cell lines, 3 of each HPV status, were investigated for radiosensitivity by clonogenic assay and modelled by response as a function of dose. Generational cultures of each cell line were developed to follow changes in radiosensitivity after repeated irradiations simulating fractionated radiation therapy. As a group, the HPV positive cell lines were more radiosensitive, but with changes following repeated fractions of dose, and modelling of response as a function of dose, both statuses displayed large radiobiological heterogeneity. These findings challenge current radiobiological assumptions of head and neck cancers as early responding tissue to radiation and may go some way in explaining difficulties reaching consensus in stratification of treatment by HPV status. Consequently, results from this study do not support stratifying radiation therapy by HPV status. MDPI 2020-07-27 /pmc/articles/PMC7464531/ /pubmed/32727072 http://dx.doi.org/10.3390/cells9081788 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reid, Paul
Staudacher, Alexander H.
Marcu, Loredana G.
Olver, Ian
Moghaddasi, Leyla
Brown, Michael P.
Li, Yanrui
Bezak, Eva
Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title_full Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title_fullStr Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title_full_unstemmed Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title_short Intrinsic Radiosensitivity Is Not the Determining Factor in Treatment Response Differences between HPV Negative and HPV Positive Head and Neck Cancers
title_sort intrinsic radiosensitivity is not the determining factor in treatment response differences between hpv negative and hpv positive head and neck cancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464531/
https://www.ncbi.nlm.nih.gov/pubmed/32727072
http://dx.doi.org/10.3390/cells9081788
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