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Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation

Extracorporeal membrane oxygenation (ECMO) is an extracorporeal circulation used to manage patients with severe circulatory or respiratory failure. It is associated with both high bleeding and thrombosis risks, mainly as a result of biomaterial/blood interface phenomena, high shear stress, and compl...

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Autores principales: Mansour, Alexandre, Roussel, Mikael, Gaussem, Pascale, Nédelec-Gac, Fabienne, Pontis, Adeline, Flécher, Erwan, Bachelot-Loza, Christilla, Gouin-Thibault, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464627/
https://www.ncbi.nlm.nih.gov/pubmed/32718096
http://dx.doi.org/10.3390/jcm9082361
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author Mansour, Alexandre
Roussel, Mikael
Gaussem, Pascale
Nédelec-Gac, Fabienne
Pontis, Adeline
Flécher, Erwan
Bachelot-Loza, Christilla
Gouin-Thibault, Isabelle
author_facet Mansour, Alexandre
Roussel, Mikael
Gaussem, Pascale
Nédelec-Gac, Fabienne
Pontis, Adeline
Flécher, Erwan
Bachelot-Loza, Christilla
Gouin-Thibault, Isabelle
author_sort Mansour, Alexandre
collection PubMed
description Extracorporeal membrane oxygenation (ECMO) is an extracorporeal circulation used to manage patients with severe circulatory or respiratory failure. It is associated with both high bleeding and thrombosis risks, mainly as a result of biomaterial/blood interface phenomena, high shear stress, and complex inflammatory response involving the activation of coagulation and complement systems, endothelial cells, leukocytes, and platelets. Besides their critical role in hemostasis, platelets are important players in inflammatory reactions, especially due to their ability to bind and activate leukocytes. Hence, we reviewed studies on platelet function of ECMO patients. Moreover, we addressed the issue of platelet–leukocyte aggregates (PLAs), which is a key step in both platelet and leukocyte activation, and deserves to be investigated in these patients. A reduced expression of GPIb and GPVI was found under ECMO therapy, due to the shedding processes. However, defective platelet aggregation is inconsistently reported and is still not clearly defined. Due to the high susceptibility of PLAs to pre-analytical conditions, defining and strictly adhering to a rigorous laboratory methodology is essential for reliable and reproducible results, especially in the setting of complex inflammatory situations like ECMO. We provide results on sample preparation and flow cytometric whole blood evaluation of circulating PLAs.
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spelling pubmed-74646272020-09-04 Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation Mansour, Alexandre Roussel, Mikael Gaussem, Pascale Nédelec-Gac, Fabienne Pontis, Adeline Flécher, Erwan Bachelot-Loza, Christilla Gouin-Thibault, Isabelle J Clin Med Review Extracorporeal membrane oxygenation (ECMO) is an extracorporeal circulation used to manage patients with severe circulatory or respiratory failure. It is associated with both high bleeding and thrombosis risks, mainly as a result of biomaterial/blood interface phenomena, high shear stress, and complex inflammatory response involving the activation of coagulation and complement systems, endothelial cells, leukocytes, and platelets. Besides their critical role in hemostasis, platelets are important players in inflammatory reactions, especially due to their ability to bind and activate leukocytes. Hence, we reviewed studies on platelet function of ECMO patients. Moreover, we addressed the issue of platelet–leukocyte aggregates (PLAs), which is a key step in both platelet and leukocyte activation, and deserves to be investigated in these patients. A reduced expression of GPIb and GPVI was found under ECMO therapy, due to the shedding processes. However, defective platelet aggregation is inconsistently reported and is still not clearly defined. Due to the high susceptibility of PLAs to pre-analytical conditions, defining and strictly adhering to a rigorous laboratory methodology is essential for reliable and reproducible results, especially in the setting of complex inflammatory situations like ECMO. We provide results on sample preparation and flow cytometric whole blood evaluation of circulating PLAs. MDPI 2020-07-23 /pmc/articles/PMC7464627/ /pubmed/32718096 http://dx.doi.org/10.3390/jcm9082361 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Mansour, Alexandre
Roussel, Mikael
Gaussem, Pascale
Nédelec-Gac, Fabienne
Pontis, Adeline
Flécher, Erwan
Bachelot-Loza, Christilla
Gouin-Thibault, Isabelle
Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title_full Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title_fullStr Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title_full_unstemmed Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title_short Platelet Functions During Extracorporeal Membrane Oxygenation. Platelet–Leukocyte Aggregates Analyzed by Flow Cytometry as a Promising Tool to Monitor Platelet Activation
title_sort platelet functions during extracorporeal membrane oxygenation. platelet–leukocyte aggregates analyzed by flow cytometry as a promising tool to monitor platelet activation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464627/
https://www.ncbi.nlm.nih.gov/pubmed/32718096
http://dx.doi.org/10.3390/jcm9082361
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