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On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins

Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle...

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Detalles Bibliográficos
Autores principales: Zhang, Youjun, Fernie, Alisdair R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464641/
https://www.ncbi.nlm.nih.gov/pubmed/32722450
http://dx.doi.org/10.3390/biom10081107
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author Zhang, Youjun
Fernie, Alisdair R.
author_facet Zhang, Youjun
Fernie, Alisdair R.
author_sort Zhang, Youjun
collection PubMed
description Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle matrices, but also at membrane surfaces where, for example, receptor complexes, as well as those of key metabolic pathways, are common. Transporters also frequently come up in lists of interacting proteins, for example, binding proteins that catalyze the production of their substrates or that act as relays within signal transduction cascades. In this review, we provide an update of technologies that are used in the study of such interactions with mitochondrial transport proteins, highlighting the difficulties that arise in their use for membrane proteins and discussing our current understanding of the biological function of such interactions.
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spelling pubmed-74646412020-09-04 On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins Zhang, Youjun Fernie, Alisdair R. Biomolecules Review Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle matrices, but also at membrane surfaces where, for example, receptor complexes, as well as those of key metabolic pathways, are common. Transporters also frequently come up in lists of interacting proteins, for example, binding proteins that catalyze the production of their substrates or that act as relays within signal transduction cascades. In this review, we provide an update of technologies that are used in the study of such interactions with mitochondrial transport proteins, highlighting the difficulties that arise in their use for membrane proteins and discussing our current understanding of the biological function of such interactions. MDPI 2020-07-25 /pmc/articles/PMC7464641/ /pubmed/32722450 http://dx.doi.org/10.3390/biom10081107 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhang, Youjun
Fernie, Alisdair R.
On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title_full On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title_fullStr On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title_full_unstemmed On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title_short On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
title_sort on the detection and functional significance of the protein–protein interactions of mitochondrial transport proteins
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464641/
https://www.ncbi.nlm.nih.gov/pubmed/32722450
http://dx.doi.org/10.3390/biom10081107
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