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On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins
Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464641/ https://www.ncbi.nlm.nih.gov/pubmed/32722450 http://dx.doi.org/10.3390/biom10081107 |
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author | Zhang, Youjun Fernie, Alisdair R. |
author_facet | Zhang, Youjun Fernie, Alisdair R. |
author_sort | Zhang, Youjun |
collection | PubMed |
description | Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle matrices, but also at membrane surfaces where, for example, receptor complexes, as well as those of key metabolic pathways, are common. Transporters also frequently come up in lists of interacting proteins, for example, binding proteins that catalyze the production of their substrates or that act as relays within signal transduction cascades. In this review, we provide an update of technologies that are used in the study of such interactions with mitochondrial transport proteins, highlighting the difficulties that arise in their use for membrane proteins and discussing our current understanding of the biological function of such interactions. |
format | Online Article Text |
id | pubmed-7464641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74646412020-09-04 On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins Zhang, Youjun Fernie, Alisdair R. Biomolecules Review Protein–protein assemblies are highly prevalent in all living cells. Considerable evidence has recently accumulated suggesting that particularly transient association/dissociation of proteins represent an important means of regulation of metabolism. This is true not only in the cytosol and organelle matrices, but also at membrane surfaces where, for example, receptor complexes, as well as those of key metabolic pathways, are common. Transporters also frequently come up in lists of interacting proteins, for example, binding proteins that catalyze the production of their substrates or that act as relays within signal transduction cascades. In this review, we provide an update of technologies that are used in the study of such interactions with mitochondrial transport proteins, highlighting the difficulties that arise in their use for membrane proteins and discussing our current understanding of the biological function of such interactions. MDPI 2020-07-25 /pmc/articles/PMC7464641/ /pubmed/32722450 http://dx.doi.org/10.3390/biom10081107 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Zhang, Youjun Fernie, Alisdair R. On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title | On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title_full | On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title_fullStr | On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title_full_unstemmed | On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title_short | On the Detection and Functional Significance of the Protein–Protein Interactions of Mitochondrial Transport Proteins |
title_sort | on the detection and functional significance of the protein–protein interactions of mitochondrial transport proteins |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464641/ https://www.ncbi.nlm.nih.gov/pubmed/32722450 http://dx.doi.org/10.3390/biom10081107 |
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