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Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis

The mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017–2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by...

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Autores principales: Ruiz-Ripa, Laura, Feßler, Andrea T., Hanke, Dennis, Eichhorn, Inga, Azcona-Gutiérrez, José Manuel, Pérez-Moreno, Mar Olga, Seral, Cristina, Aspiroz, Carmen, Alonso, Carla Andrea, Torres, Luis, Alós, Juan-Ignacio, Schwarz, Stefan, Torres, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464793/
https://www.ncbi.nlm.nih.gov/pubmed/32751552
http://dx.doi.org/10.3390/microorganisms8081155
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author Ruiz-Ripa, Laura
Feßler, Andrea T.
Hanke, Dennis
Eichhorn, Inga
Azcona-Gutiérrez, José Manuel
Pérez-Moreno, Mar Olga
Seral, Cristina
Aspiroz, Carmen
Alonso, Carla Andrea
Torres, Luis
Alós, Juan-Ignacio
Schwarz, Stefan
Torres, Carmen
author_facet Ruiz-Ripa, Laura
Feßler, Andrea T.
Hanke, Dennis
Eichhorn, Inga
Azcona-Gutiérrez, José Manuel
Pérez-Moreno, Mar Olga
Seral, Cristina
Aspiroz, Carmen
Alonso, Carla Andrea
Torres, Luis
Alós, Juan-Ignacio
Schwarz, Stefan
Torres, Carmen
author_sort Ruiz-Ripa, Laura
collection PubMed
description The mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017–2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by PCR and amplicon sequencing. Moreover, the susceptibility to 18 antimicrobial agents was investigated, and the respective molecular background was elucidated by PCR-amplicon sequencing and whole genome sequencing. The transferability of the linezolid resistance genes was evaluated by filter-mating experiments. The optrA gene was detected in all 13 E. faecalis isolates; and one optrA-positive isolate also carried the recently described cfr(D) gene. Moreover, one E. faecalis isolate displayed the nucleotide mutation G2576T in the 23S rDNA. This mutation was also present in all six E. faecium isolates. All linezolid-resistant enterococci showed a multiresistance phenotype and harbored several antimicrobial resistance genes, as well as many virulence determinants. The fexA gene was located upstream of the optrA gene in 12 of the E. faecalis isolates. Moreover, an erm(A)-like gene was located downstream of optrA in two isolates recovered from the same hospital. The optrA gene was transferable in all but one E. faecalis isolates, in all cases along with the fexA gene. The cfr(D) gene was not transferable. The presence of optrA and mutations in the 23S rDNA are the main mechanisms of linezolid resistance among E. faecalis and E. faecium, respectively. We report the first description of the cfr(D) gene in E. faecalis. The presence of the optrA and cfr(D) genes in Spanish hospitals is a public health concern.
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spelling pubmed-74647932020-09-04 Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis Ruiz-Ripa, Laura Feßler, Andrea T. Hanke, Dennis Eichhorn, Inga Azcona-Gutiérrez, José Manuel Pérez-Moreno, Mar Olga Seral, Cristina Aspiroz, Carmen Alonso, Carla Andrea Torres, Luis Alós, Juan-Ignacio Schwarz, Stefan Torres, Carmen Microorganisms Article The mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017–2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by PCR and amplicon sequencing. Moreover, the susceptibility to 18 antimicrobial agents was investigated, and the respective molecular background was elucidated by PCR-amplicon sequencing and whole genome sequencing. The transferability of the linezolid resistance genes was evaluated by filter-mating experiments. The optrA gene was detected in all 13 E. faecalis isolates; and one optrA-positive isolate also carried the recently described cfr(D) gene. Moreover, one E. faecalis isolate displayed the nucleotide mutation G2576T in the 23S rDNA. This mutation was also present in all six E. faecium isolates. All linezolid-resistant enterococci showed a multiresistance phenotype and harbored several antimicrobial resistance genes, as well as many virulence determinants. The fexA gene was located upstream of the optrA gene in 12 of the E. faecalis isolates. Moreover, an erm(A)-like gene was located downstream of optrA in two isolates recovered from the same hospital. The optrA gene was transferable in all but one E. faecalis isolates, in all cases along with the fexA gene. The cfr(D) gene was not transferable. The presence of optrA and mutations in the 23S rDNA are the main mechanisms of linezolid resistance among E. faecalis and E. faecium, respectively. We report the first description of the cfr(D) gene in E. faecalis. The presence of the optrA and cfr(D) genes in Spanish hospitals is a public health concern. MDPI 2020-07-30 /pmc/articles/PMC7464793/ /pubmed/32751552 http://dx.doi.org/10.3390/microorganisms8081155 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruiz-Ripa, Laura
Feßler, Andrea T.
Hanke, Dennis
Eichhorn, Inga
Azcona-Gutiérrez, José Manuel
Pérez-Moreno, Mar Olga
Seral, Cristina
Aspiroz, Carmen
Alonso, Carla Andrea
Torres, Luis
Alós, Juan-Ignacio
Schwarz, Stefan
Torres, Carmen
Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title_full Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title_fullStr Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title_full_unstemmed Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title_short Mechanisms of Linezolid Resistance Among Enterococci of Clinical Origin in Spain—Detection of optrA- and cfr(D)-Carrying E. faecalis
title_sort mechanisms of linezolid resistance among enterococci of clinical origin in spain—detection of optra- and cfr(d)-carrying e. faecalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464793/
https://www.ncbi.nlm.nih.gov/pubmed/32751552
http://dx.doi.org/10.3390/microorganisms8081155
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