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circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response
Deep sequencing technologies have revealed the once uncharted non-coding transcriptome of circular RNAs (circRNAs). Despite the lack of protein-coding potential, these unorthodox yet highly stable RNA species are known to act as critical gene regulatory hubs, particularly in malignancies. However, t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464896/ https://www.ncbi.nlm.nih.gov/pubmed/32785098 http://dx.doi.org/10.3390/cells9081868 |
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author | Chen, Yi-Tung Chang, Ian Yi-Feng Kan, Chia-Hua Liu, Yu-Hao Kuo, Yu-Ping Tseng, Hsin-Hao Chen, Hsing-Chun Liu, Hsuan Chang, Yu-Sun Yu, Jau-Song Chang, Kai-Ping Tan, Bertrand Chin-Ming |
author_facet | Chen, Yi-Tung Chang, Ian Yi-Feng Kan, Chia-Hua Liu, Yu-Hao Kuo, Yu-Ping Tseng, Hsin-Hao Chen, Hsing-Chun Liu, Hsuan Chang, Yu-Sun Yu, Jau-Song Chang, Kai-Ping Tan, Bertrand Chin-Ming |
author_sort | Chen, Yi-Tung |
collection | PubMed |
description | Deep sequencing technologies have revealed the once uncharted non-coding transcriptome of circular RNAs (circRNAs). Despite the lack of protein-coding potential, these unorthodox yet highly stable RNA species are known to act as critical gene regulatory hubs, particularly in malignancies. However, their mechanistic implications in tumor outcome and translational potential have not been fully resolved. Using RNA-seq data, we profiled the circRNAomes of tumor specimens derived from oral squamous cell carcinoma (OSCC), which is a prevalently diagnosed cancer with a persistently low survival rate. We further catalogued dysregulated circRNAs in connection with tumorigenic progression. Using comprehensive bioinformatics analyses focused on co-expression maps and miRNA-interaction networks, we delineated the regulatory networks that are centered on circRNAs. Interestingly, we identified a tumor-associated, pro-tumorigenic circRNA, named circFLNB, that was implicated in maintaining several tumor-associated phenotypes in vitro and in vivo. Correspondingly, transcriptome profiling of circFLNB-knockdown cells showed alterations in tumor-related genes. Integrated in silico analyses further deciphered the circFLNB-targeted gene network. Together, our current study demarcates the OSCC-associated circRNAome, and unveils a novel circRNA circuit with functional implication in OSCC progression. These systems-based findings broaden mechanistic understanding of oral malignancies and raise new prospects for translational medicine. |
format | Online Article Text |
id | pubmed-7464896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74648962020-09-04 circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response Chen, Yi-Tung Chang, Ian Yi-Feng Kan, Chia-Hua Liu, Yu-Hao Kuo, Yu-Ping Tseng, Hsin-Hao Chen, Hsing-Chun Liu, Hsuan Chang, Yu-Sun Yu, Jau-Song Chang, Kai-Ping Tan, Bertrand Chin-Ming Cells Article Deep sequencing technologies have revealed the once uncharted non-coding transcriptome of circular RNAs (circRNAs). Despite the lack of protein-coding potential, these unorthodox yet highly stable RNA species are known to act as critical gene regulatory hubs, particularly in malignancies. However, their mechanistic implications in tumor outcome and translational potential have not been fully resolved. Using RNA-seq data, we profiled the circRNAomes of tumor specimens derived from oral squamous cell carcinoma (OSCC), which is a prevalently diagnosed cancer with a persistently low survival rate. We further catalogued dysregulated circRNAs in connection with tumorigenic progression. Using comprehensive bioinformatics analyses focused on co-expression maps and miRNA-interaction networks, we delineated the regulatory networks that are centered on circRNAs. Interestingly, we identified a tumor-associated, pro-tumorigenic circRNA, named circFLNB, that was implicated in maintaining several tumor-associated phenotypes in vitro and in vivo. Correspondingly, transcriptome profiling of circFLNB-knockdown cells showed alterations in tumor-related genes. Integrated in silico analyses further deciphered the circFLNB-targeted gene network. Together, our current study demarcates the OSCC-associated circRNAome, and unveils a novel circRNA circuit with functional implication in OSCC progression. These systems-based findings broaden mechanistic understanding of oral malignancies and raise new prospects for translational medicine. MDPI 2020-08-10 /pmc/articles/PMC7464896/ /pubmed/32785098 http://dx.doi.org/10.3390/cells9081868 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yi-Tung Chang, Ian Yi-Feng Kan, Chia-Hua Liu, Yu-Hao Kuo, Yu-Ping Tseng, Hsin-Hao Chen, Hsing-Chun Liu, Hsuan Chang, Yu-Sun Yu, Jau-Song Chang, Kai-Ping Tan, Bertrand Chin-Ming circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title | circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title_full | circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title_fullStr | circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title_full_unstemmed | circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title_short | circRNAome Profiling in Oral Carcinoma Unveils a Novel circFLNB that Mediates Tumour Growth-Regulating Transcriptional Response |
title_sort | circrnaome profiling in oral carcinoma unveils a novel circflnb that mediates tumour growth-regulating transcriptional response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464896/ https://www.ncbi.nlm.nih.gov/pubmed/32785098 http://dx.doi.org/10.3390/cells9081868 |
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