Cargando…

A Transmembrane Histidine Kinase Functions as a pH Sensor

The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—als...

Descripción completa

Detalles Bibliográficos
Autores principales: Bortolotti, Ana, Vazquez, Daniela Belén, Almada, Juan Cruz, Inda, Maria Eugenia, Drusin, Salvador Iván, Villalba, Juan Manuel, Moreno, Diego M., Ruysschaert, Jean Marie, Cybulski, Larisa Estefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465017/
https://www.ncbi.nlm.nih.gov/pubmed/32823946
http://dx.doi.org/10.3390/biom10081183
_version_ 1783577494910140416
author Bortolotti, Ana
Vazquez, Daniela Belén
Almada, Juan Cruz
Inda, Maria Eugenia
Drusin, Salvador Iván
Villalba, Juan Manuel
Moreno, Diego M.
Ruysschaert, Jean Marie
Cybulski, Larisa Estefania
author_facet Bortolotti, Ana
Vazquez, Daniela Belén
Almada, Juan Cruz
Inda, Maria Eugenia
Drusin, Salvador Iván
Villalba, Juan Manuel
Moreno, Diego M.
Ruysschaert, Jean Marie
Cybulski, Larisa Estefania
author_sort Bortolotti, Ana
collection PubMed
description The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—also responds to pH and studied the mechanism of pH sensing. We propose that a helix linking the transmembrane region with the cytoplasmic catalytic domain is involved in pH sensing. This helix contains several glutamate, lysine, and arginine residues At neutral pH, the linker forms an alpha helix that is stabilized by hydrogen bonds in the i, i + 4 register and thus favors the kinase state. At low pH, protonation of glutamate residues breaks salt bridges, which results in helix destabilization and interruption of signaling. This mechanism inhibits unsaturated fatty acid synthesis and rigidifies the membrane when Bacillus grows in acidic conditions.
format Online
Article
Text
id pubmed-7465017
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74650172020-09-04 A Transmembrane Histidine Kinase Functions as a pH Sensor Bortolotti, Ana Vazquez, Daniela Belén Almada, Juan Cruz Inda, Maria Eugenia Drusin, Salvador Iván Villalba, Juan Manuel Moreno, Diego M. Ruysschaert, Jean Marie Cybulski, Larisa Estefania Biomolecules Article The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—also responds to pH and studied the mechanism of pH sensing. We propose that a helix linking the transmembrane region with the cytoplasmic catalytic domain is involved in pH sensing. This helix contains several glutamate, lysine, and arginine residues At neutral pH, the linker forms an alpha helix that is stabilized by hydrogen bonds in the i, i + 4 register and thus favors the kinase state. At low pH, protonation of glutamate residues breaks salt bridges, which results in helix destabilization and interruption of signaling. This mechanism inhibits unsaturated fatty acid synthesis and rigidifies the membrane when Bacillus grows in acidic conditions. MDPI 2020-08-14 /pmc/articles/PMC7465017/ /pubmed/32823946 http://dx.doi.org/10.3390/biom10081183 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bortolotti, Ana
Vazquez, Daniela Belén
Almada, Juan Cruz
Inda, Maria Eugenia
Drusin, Salvador Iván
Villalba, Juan Manuel
Moreno, Diego M.
Ruysschaert, Jean Marie
Cybulski, Larisa Estefania
A Transmembrane Histidine Kinase Functions as a pH Sensor
title A Transmembrane Histidine Kinase Functions as a pH Sensor
title_full A Transmembrane Histidine Kinase Functions as a pH Sensor
title_fullStr A Transmembrane Histidine Kinase Functions as a pH Sensor
title_full_unstemmed A Transmembrane Histidine Kinase Functions as a pH Sensor
title_short A Transmembrane Histidine Kinase Functions as a pH Sensor
title_sort transmembrane histidine kinase functions as a ph sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465017/
https://www.ncbi.nlm.nih.gov/pubmed/32823946
http://dx.doi.org/10.3390/biom10081183
work_keys_str_mv AT bortolottiana atransmembranehistidinekinasefunctionsasaphsensor
AT vazquezdanielabelen atransmembranehistidinekinasefunctionsasaphsensor
AT almadajuancruz atransmembranehistidinekinasefunctionsasaphsensor
AT indamariaeugenia atransmembranehistidinekinasefunctionsasaphsensor
AT drusinsalvadorivan atransmembranehistidinekinasefunctionsasaphsensor
AT villalbajuanmanuel atransmembranehistidinekinasefunctionsasaphsensor
AT morenodiegom atransmembranehistidinekinasefunctionsasaphsensor
AT ruysschaertjeanmarie atransmembranehistidinekinasefunctionsasaphsensor
AT cybulskilarisaestefania atransmembranehistidinekinasefunctionsasaphsensor
AT bortolottiana transmembranehistidinekinasefunctionsasaphsensor
AT vazquezdanielabelen transmembranehistidinekinasefunctionsasaphsensor
AT almadajuancruz transmembranehistidinekinasefunctionsasaphsensor
AT indamariaeugenia transmembranehistidinekinasefunctionsasaphsensor
AT drusinsalvadorivan transmembranehistidinekinasefunctionsasaphsensor
AT villalbajuanmanuel transmembranehistidinekinasefunctionsasaphsensor
AT morenodiegom transmembranehistidinekinasefunctionsasaphsensor
AT ruysschaertjeanmarie transmembranehistidinekinasefunctionsasaphsensor
AT cybulskilarisaestefania transmembranehistidinekinasefunctionsasaphsensor