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A Transmembrane Histidine Kinase Functions as a pH Sensor
The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—als...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465017/ https://www.ncbi.nlm.nih.gov/pubmed/32823946 http://dx.doi.org/10.3390/biom10081183 |
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author | Bortolotti, Ana Vazquez, Daniela Belén Almada, Juan Cruz Inda, Maria Eugenia Drusin, Salvador Iván Villalba, Juan Manuel Moreno, Diego M. Ruysschaert, Jean Marie Cybulski, Larisa Estefania |
author_facet | Bortolotti, Ana Vazquez, Daniela Belén Almada, Juan Cruz Inda, Maria Eugenia Drusin, Salvador Iván Villalba, Juan Manuel Moreno, Diego M. Ruysschaert, Jean Marie Cybulski, Larisa Estefania |
author_sort | Bortolotti, Ana |
collection | PubMed |
description | The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—also responds to pH and studied the mechanism of pH sensing. We propose that a helix linking the transmembrane region with the cytoplasmic catalytic domain is involved in pH sensing. This helix contains several glutamate, lysine, and arginine residues At neutral pH, the linker forms an alpha helix that is stabilized by hydrogen bonds in the i, i + 4 register and thus favors the kinase state. At low pH, protonation of glutamate residues breaks salt bridges, which results in helix destabilization and interruption of signaling. This mechanism inhibits unsaturated fatty acid synthesis and rigidifies the membrane when Bacillus grows in acidic conditions. |
format | Online Article Text |
id | pubmed-7465017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74650172020-09-04 A Transmembrane Histidine Kinase Functions as a pH Sensor Bortolotti, Ana Vazquez, Daniela Belén Almada, Juan Cruz Inda, Maria Eugenia Drusin, Salvador Iván Villalba, Juan Manuel Moreno, Diego M. Ruysschaert, Jean Marie Cybulski, Larisa Estefania Biomolecules Article The two-component system DesK-DesR regulates the synthesis of unsaturated fatty acids in the soil bacteria Bacillus subtilis. This system is activated at low temperature and maintains membrane lipid fluidity upon temperature variations. Here, we found that DesK—the transmembrane histidine kinase—also responds to pH and studied the mechanism of pH sensing. We propose that a helix linking the transmembrane region with the cytoplasmic catalytic domain is involved in pH sensing. This helix contains several glutamate, lysine, and arginine residues At neutral pH, the linker forms an alpha helix that is stabilized by hydrogen bonds in the i, i + 4 register and thus favors the kinase state. At low pH, protonation of glutamate residues breaks salt bridges, which results in helix destabilization and interruption of signaling. This mechanism inhibits unsaturated fatty acid synthesis and rigidifies the membrane when Bacillus grows in acidic conditions. MDPI 2020-08-14 /pmc/articles/PMC7465017/ /pubmed/32823946 http://dx.doi.org/10.3390/biom10081183 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bortolotti, Ana Vazquez, Daniela Belén Almada, Juan Cruz Inda, Maria Eugenia Drusin, Salvador Iván Villalba, Juan Manuel Moreno, Diego M. Ruysschaert, Jean Marie Cybulski, Larisa Estefania A Transmembrane Histidine Kinase Functions as a pH Sensor |
title | A Transmembrane Histidine Kinase Functions as a pH Sensor |
title_full | A Transmembrane Histidine Kinase Functions as a pH Sensor |
title_fullStr | A Transmembrane Histidine Kinase Functions as a pH Sensor |
title_full_unstemmed | A Transmembrane Histidine Kinase Functions as a pH Sensor |
title_short | A Transmembrane Histidine Kinase Functions as a pH Sensor |
title_sort | transmembrane histidine kinase functions as a ph sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465017/ https://www.ncbi.nlm.nih.gov/pubmed/32823946 http://dx.doi.org/10.3390/biom10081183 |
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