Cargando…
Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice
Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genoty...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465205/ https://www.ncbi.nlm.nih.gov/pubmed/32796677 http://dx.doi.org/10.3390/antiox9080733 |
_version_ | 1783577536693796864 |
---|---|
author | Leyva-Jiménez, Francisco Javier Ruiz-Malagón, Antonio Jesús Molina-Tijeras, José Alberto Diez-Echave, Patricia Vezza, Teresa Hidalgo-García, Laura Lozano-Sánchez, Jesús Arráez-Román, David Cenis, José Luis Lozano-Pérez, Antonio Abel Rodríguez-Nogales, Alba Segura-Carretero, Antonio Gálvez, Julio |
author_facet | Leyva-Jiménez, Francisco Javier Ruiz-Malagón, Antonio Jesús Molina-Tijeras, José Alberto Diez-Echave, Patricia Vezza, Teresa Hidalgo-García, Laura Lozano-Sánchez, Jesús Arráez-Román, David Cenis, José Luis Lozano-Pérez, Antonio Abel Rodríguez-Nogales, Alba Segura-Carretero, Antonio Gálvez, Julio |
author_sort | Leyva-Jiménez, Francisco Javier |
collection | PubMed |
description | Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders. |
format | Online Article Text |
id | pubmed-7465205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74652052020-09-04 Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice Leyva-Jiménez, Francisco Javier Ruiz-Malagón, Antonio Jesús Molina-Tijeras, José Alberto Diez-Echave, Patricia Vezza, Teresa Hidalgo-García, Laura Lozano-Sánchez, Jesús Arráez-Román, David Cenis, José Luis Lozano-Pérez, Antonio Abel Rodríguez-Nogales, Alba Segura-Carretero, Antonio Gálvez, Julio Antioxidants (Basel) Article Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders. MDPI 2020-08-11 /pmc/articles/PMC7465205/ /pubmed/32796677 http://dx.doi.org/10.3390/antiox9080733 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Leyva-Jiménez, Francisco Javier Ruiz-Malagón, Antonio Jesús Molina-Tijeras, José Alberto Diez-Echave, Patricia Vezza, Teresa Hidalgo-García, Laura Lozano-Sánchez, Jesús Arráez-Román, David Cenis, José Luis Lozano-Pérez, Antonio Abel Rodríguez-Nogales, Alba Segura-Carretero, Antonio Gálvez, Julio Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title_full | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title_fullStr | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title_full_unstemmed | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title_short | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice |
title_sort | comparative study of the antioxidant and anti-inflammatory effects of leaf extracts from four different morus alba genotypes in high fat diet-induced obesity in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465205/ https://www.ncbi.nlm.nih.gov/pubmed/32796677 http://dx.doi.org/10.3390/antiox9080733 |
work_keys_str_mv | AT leyvajimenezfranciscojavier comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT ruizmalagonantoniojesus comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT molinatijerasjosealberto comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT diezechavepatricia comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT vezzateresa comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT hidalgogarcialaura comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT lozanosanchezjesus comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT arraezromandavid comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT cenisjoseluis comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT lozanoperezantonioabel comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT rodrigueznogalesalba comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT seguracarreteroantonio comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice AT galvezjulio comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentmorusalbagenotypesinhighfatdietinducedobesityinmice |