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Generation of Marker-Free pbd-2 Knock-in Pigs Using the CRISPR/Cas9 and Cre/loxP Systems

Porcine β-defensin 2 (PBD-2), expressed by different tissues of pigs, is a multifunctional cationic peptide with antimicrobial, immunomodulatory and growth-promoting abilities. As the latest generation of genome-editing tool, CRISPR/Cas9 system makes it possible to enhance the expression of PBD-2 in...

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Detalles Bibliográficos
Autores principales:	Huang, Jing, Wang, Antian, Huang, Chao, Sun, Yufan, Song, Bingxiao, Zhou, Rui, Li, Lu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465224/ https://www.ncbi.nlm.nih.gov/pubmed/32824735 http://dx.doi.org/10.3390/genes11080951

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