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Microbial Signature in Adipose Tissue of Crohn’s Disease Patients

Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis mig...

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Autores principales: Serena, Carolina, Queipo-Ortuño, Maribel, Millan, Monica, Sanchez-Alcoholado, Lidia, Caro, Aleidis, Espina, Beatriz, Menacho, Margarita, Bautista, Michelle, Monfort-Ferré, Diandra, Terrón-Puig, Margarida, Núñez-Roa, Catalina, Maymó-Masip, Elsa, Rodriguez, M. Mar, Tinahones, Francisco J., Espin, Eloy, Martí, Marc, Fernández-Veledo, Sonia, Vendrell, Joan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465250/
https://www.ncbi.nlm.nih.gov/pubmed/32751800
http://dx.doi.org/10.3390/jcm9082448
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author Serena, Carolina
Queipo-Ortuño, Maribel
Millan, Monica
Sanchez-Alcoholado, Lidia
Caro, Aleidis
Espina, Beatriz
Menacho, Margarita
Bautista, Michelle
Monfort-Ferré, Diandra
Terrón-Puig, Margarida
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Rodriguez, M. Mar
Tinahones, Francisco J.
Espin, Eloy
Martí, Marc
Fernández-Veledo, Sonia
Vendrell, Joan
author_facet Serena, Carolina
Queipo-Ortuño, Maribel
Millan, Monica
Sanchez-Alcoholado, Lidia
Caro, Aleidis
Espina, Beatriz
Menacho, Margarita
Bautista, Michelle
Monfort-Ferré, Diandra
Terrón-Puig, Margarida
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Rodriguez, M. Mar
Tinahones, Francisco J.
Espin, Eloy
Martí, Marc
Fernández-Veledo, Sonia
Vendrell, Joan
author_sort Serena, Carolina
collection PubMed
description Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.
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spelling pubmed-74652502020-09-04 Microbial Signature in Adipose Tissue of Crohn’s Disease Patients Serena, Carolina Queipo-Ortuño, Maribel Millan, Monica Sanchez-Alcoholado, Lidia Caro, Aleidis Espina, Beatriz Menacho, Margarita Bautista, Michelle Monfort-Ferré, Diandra Terrón-Puig, Margarida Núñez-Roa, Catalina Maymó-Masip, Elsa Rodriguez, M. Mar Tinahones, Francisco J. Espin, Eloy Martí, Marc Fernández-Veledo, Sonia Vendrell, Joan J Clin Med Article Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. MDPI 2020-07-31 /pmc/articles/PMC7465250/ /pubmed/32751800 http://dx.doi.org/10.3390/jcm9082448 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Serena, Carolina
Queipo-Ortuño, Maribel
Millan, Monica
Sanchez-Alcoholado, Lidia
Caro, Aleidis
Espina, Beatriz
Menacho, Margarita
Bautista, Michelle
Monfort-Ferré, Diandra
Terrón-Puig, Margarida
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Rodriguez, M. Mar
Tinahones, Francisco J.
Espin, Eloy
Martí, Marc
Fernández-Veledo, Sonia
Vendrell, Joan
Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title_full Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title_fullStr Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title_full_unstemmed Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title_short Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
title_sort microbial signature in adipose tissue of crohn’s disease patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465250/
https://www.ncbi.nlm.nih.gov/pubmed/32751800
http://dx.doi.org/10.3390/jcm9082448
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