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Microbial Signature in Adipose Tissue of Crohn’s Disease Patients
Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis mig...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465250/ https://www.ncbi.nlm.nih.gov/pubmed/32751800 http://dx.doi.org/10.3390/jcm9082448 |
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author | Serena, Carolina Queipo-Ortuño, Maribel Millan, Monica Sanchez-Alcoholado, Lidia Caro, Aleidis Espina, Beatriz Menacho, Margarita Bautista, Michelle Monfort-Ferré, Diandra Terrón-Puig, Margarida Núñez-Roa, Catalina Maymó-Masip, Elsa Rodriguez, M. Mar Tinahones, Francisco J. Espin, Eloy Martí, Marc Fernández-Veledo, Sonia Vendrell, Joan |
author_facet | Serena, Carolina Queipo-Ortuño, Maribel Millan, Monica Sanchez-Alcoholado, Lidia Caro, Aleidis Espina, Beatriz Menacho, Margarita Bautista, Michelle Monfort-Ferré, Diandra Terrón-Puig, Margarida Núñez-Roa, Catalina Maymó-Masip, Elsa Rodriguez, M. Mar Tinahones, Francisco J. Espin, Eloy Martí, Marc Fernández-Veledo, Sonia Vendrell, Joan |
author_sort | Serena, Carolina |
collection | PubMed |
description | Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. |
format | Online Article Text |
id | pubmed-7465250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74652502020-09-04 Microbial Signature in Adipose Tissue of Crohn’s Disease Patients Serena, Carolina Queipo-Ortuño, Maribel Millan, Monica Sanchez-Alcoholado, Lidia Caro, Aleidis Espina, Beatriz Menacho, Margarita Bautista, Michelle Monfort-Ferré, Diandra Terrón-Puig, Margarida Núñez-Roa, Catalina Maymó-Masip, Elsa Rodriguez, M. Mar Tinahones, Francisco J. Espin, Eloy Martí, Marc Fernández-Veledo, Sonia Vendrell, Joan J Clin Med Article Crohn’s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. MDPI 2020-07-31 /pmc/articles/PMC7465250/ /pubmed/32751800 http://dx.doi.org/10.3390/jcm9082448 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Serena, Carolina Queipo-Ortuño, Maribel Millan, Monica Sanchez-Alcoholado, Lidia Caro, Aleidis Espina, Beatriz Menacho, Margarita Bautista, Michelle Monfort-Ferré, Diandra Terrón-Puig, Margarida Núñez-Roa, Catalina Maymó-Masip, Elsa Rodriguez, M. Mar Tinahones, Francisco J. Espin, Eloy Martí, Marc Fernández-Veledo, Sonia Vendrell, Joan Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title | Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title_full | Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title_fullStr | Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title_full_unstemmed | Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title_short | Microbial Signature in Adipose Tissue of Crohn’s Disease Patients |
title_sort | microbial signature in adipose tissue of crohn’s disease patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465250/ https://www.ncbi.nlm.nih.gov/pubmed/32751800 http://dx.doi.org/10.3390/jcm9082448 |
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