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Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle
Coxiella burnetii is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465334/ https://www.ncbi.nlm.nih.gov/pubmed/32823774 http://dx.doi.org/10.3390/microorganisms8081235 |
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author | Stellfeld, Mareike Gerlach, Claudia Richter, Ina-Gabriele Miethe, Peter Fahlbusch, Dominika Polley, Birgitta Sting, Reinhard Pfeffer, Martin Neubauer, Heinrich Mertens-Scholz, Katja |
author_facet | Stellfeld, Mareike Gerlach, Claudia Richter, Ina-Gabriele Miethe, Peter Fahlbusch, Dominika Polley, Birgitta Sting, Reinhard Pfeffer, Martin Neubauer, Heinrich Mertens-Scholz, Katja |
author_sort | Stellfeld, Mareike |
collection | PubMed |
description | Coxiella burnetii is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity must be developed. For this purpose, the C. burnetii outer membrane protein Com1 was cloned and expressed in Escherichia coli. The His-tagged recombinant protein was purified and used in an indirect enzyme-linked immunosorbent assay (ELISA). Assay performance was tested with more than 400 positive and negative sera from sheep, goats and cattle from 36 locations. Calculation of sensitivity and specificity was undertaken using receiver operating characteristic (ROC) curves. The sensitivities and specificities for sheep were 85% and 68% (optical density at 450nm, OD(450) cut-off value 0.32), for goats 94% and 77% (OD(450) cut-off value 0.23) and for cattle 71% and 70% (OD(450) cut-off value 0.18), respectively. These results correspond to excellent, outstanding and acceptable discrimination of positive and negative sera. In summary, recombinant Com1 can provide a basis for more sensitive and specific diagnostic tools in veterinary medicine. |
format | Online Article Text |
id | pubmed-7465334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74653342020-09-04 Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle Stellfeld, Mareike Gerlach, Claudia Richter, Ina-Gabriele Miethe, Peter Fahlbusch, Dominika Polley, Birgitta Sting, Reinhard Pfeffer, Martin Neubauer, Heinrich Mertens-Scholz, Katja Microorganisms Article Coxiella burnetii is the causative agent of Q fever, a zoonosis infecting domestic ruminants and humans. Currently used routine diagnostic tools offer limited sensitivity and specificity and symptomless infected animals may be missed. Therefore, diagnostic tools of higher sensitivity and specificity must be developed. For this purpose, the C. burnetii outer membrane protein Com1 was cloned and expressed in Escherichia coli. The His-tagged recombinant protein was purified and used in an indirect enzyme-linked immunosorbent assay (ELISA). Assay performance was tested with more than 400 positive and negative sera from sheep, goats and cattle from 36 locations. Calculation of sensitivity and specificity was undertaken using receiver operating characteristic (ROC) curves. The sensitivities and specificities for sheep were 85% and 68% (optical density at 450nm, OD(450) cut-off value 0.32), for goats 94% and 77% (OD(450) cut-off value 0.23) and for cattle 71% and 70% (OD(450) cut-off value 0.18), respectively. These results correspond to excellent, outstanding and acceptable discrimination of positive and negative sera. In summary, recombinant Com1 can provide a basis for more sensitive and specific diagnostic tools in veterinary medicine. MDPI 2020-08-13 /pmc/articles/PMC7465334/ /pubmed/32823774 http://dx.doi.org/10.3390/microorganisms8081235 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stellfeld, Mareike Gerlach, Claudia Richter, Ina-Gabriele Miethe, Peter Fahlbusch, Dominika Polley, Birgitta Sting, Reinhard Pfeffer, Martin Neubauer, Heinrich Mertens-Scholz, Katja Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title | Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title_full | Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title_fullStr | Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title_full_unstemmed | Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title_short | Evaluation of the Diagnostic Potential of Recombinant Coxiella burnetii Com1 in an ELISA for the Diagnosis of Q Fever in Sheep, Goats and Cattle |
title_sort | evaluation of the diagnostic potential of recombinant coxiella burnetii com1 in an elisa for the diagnosis of q fever in sheep, goats and cattle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465334/ https://www.ncbi.nlm.nih.gov/pubmed/32823774 http://dx.doi.org/10.3390/microorganisms8081235 |
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