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Proteomic Signatures of Corals from Thermodynamic Reefs

Unlike most parts of the world, coral reefs of Taiwan’s deep south have generally been spared from climate change-induced degradation. This has been linked to the oceanographically unique nature of Nanwan Bay, where intense upwelling occurs. Specifically, large-amplitude internal waves cause shifts...

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Autor principal: Mayfield, Anderson B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465421/
https://www.ncbi.nlm.nih.gov/pubmed/32752238
http://dx.doi.org/10.3390/microorganisms8081171
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author Mayfield, Anderson B.
author_facet Mayfield, Anderson B.
author_sort Mayfield, Anderson B.
collection PubMed
description Unlike most parts of the world, coral reefs of Taiwan’s deep south have generally been spared from climate change-induced degradation. This has been linked to the oceanographically unique nature of Nanwan Bay, where intense upwelling occurs. Specifically, large-amplitude internal waves cause shifts in temperature of 6–9 °C over the course of several hours, and the resident corals not only thrive under such conditions, but they have also been shown to withstand multi-month laboratory incubations at experimentally elevated temperatures. To gain insight into the sub-cellular basis of acclimation to upwelling, proteins isolated from reef corals (Seriatopora hystrix) featured in laboratory-based reciprocal transplant studies in which corals from upwelling and non-upwelling control reefs (<20 km away) were exposed to stable or variable temperature regimes were analyzed via label-based proteomics (iTRAQ). Corals exposed to their “native” temperature conditions for seven days (1) demonstrated highest growth rates and (2) were most distinct from one another with respect to their protein signatures. The latter observation was driven by the fact that two Symbiodiniaceae lipid trafficking proteins, sec1a and sec34, were marginally up-regulated in corals exposed to their native temperature conditions. Alongside the marked degree of proteomic “site fidelity” documented, this dataset sheds light on the molecular mechanisms underlying acclimatization to thermodynamically extreme conditions in situ.
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spelling pubmed-74654212020-09-04 Proteomic Signatures of Corals from Thermodynamic Reefs Mayfield, Anderson B. Microorganisms Article Unlike most parts of the world, coral reefs of Taiwan’s deep south have generally been spared from climate change-induced degradation. This has been linked to the oceanographically unique nature of Nanwan Bay, where intense upwelling occurs. Specifically, large-amplitude internal waves cause shifts in temperature of 6–9 °C over the course of several hours, and the resident corals not only thrive under such conditions, but they have also been shown to withstand multi-month laboratory incubations at experimentally elevated temperatures. To gain insight into the sub-cellular basis of acclimation to upwelling, proteins isolated from reef corals (Seriatopora hystrix) featured in laboratory-based reciprocal transplant studies in which corals from upwelling and non-upwelling control reefs (<20 km away) were exposed to stable or variable temperature regimes were analyzed via label-based proteomics (iTRAQ). Corals exposed to their “native” temperature conditions for seven days (1) demonstrated highest growth rates and (2) were most distinct from one another with respect to their protein signatures. The latter observation was driven by the fact that two Symbiodiniaceae lipid trafficking proteins, sec1a and sec34, were marginally up-regulated in corals exposed to their native temperature conditions. Alongside the marked degree of proteomic “site fidelity” documented, this dataset sheds light on the molecular mechanisms underlying acclimatization to thermodynamically extreme conditions in situ. MDPI 2020-08-01 /pmc/articles/PMC7465421/ /pubmed/32752238 http://dx.doi.org/10.3390/microorganisms8081171 Text en © 2020 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mayfield, Anderson B.
Proteomic Signatures of Corals from Thermodynamic Reefs
title Proteomic Signatures of Corals from Thermodynamic Reefs
title_full Proteomic Signatures of Corals from Thermodynamic Reefs
title_fullStr Proteomic Signatures of Corals from Thermodynamic Reefs
title_full_unstemmed Proteomic Signatures of Corals from Thermodynamic Reefs
title_short Proteomic Signatures of Corals from Thermodynamic Reefs
title_sort proteomic signatures of corals from thermodynamic reefs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465421/
https://www.ncbi.nlm.nih.gov/pubmed/32752238
http://dx.doi.org/10.3390/microorganisms8081171
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