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Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates

Dendrobium officinale, a precious herbal medicine, has been used for a long time in Chinese history. The metabolites of D. officinale, regarded as its effective components to fight diseases, are significantly affected by cultivation substrates. In this study, ultra-performance liquid chromatography...

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Autores principales: Zuo, Si-Min, Yu, Hai-Dong, Zhang, Weimin, Zhong, Qiuping, Chen, Wenxue, Chen, Weijun, Yun, Yong-Huan, Chen, Haiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465462/
https://www.ncbi.nlm.nih.gov/pubmed/32785071
http://dx.doi.org/10.3390/metabo10080325
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author Zuo, Si-Min
Yu, Hai-Dong
Zhang, Weimin
Zhong, Qiuping
Chen, Wenxue
Chen, Weijun
Yun, Yong-Huan
Chen, Haiming
author_facet Zuo, Si-Min
Yu, Hai-Dong
Zhang, Weimin
Zhong, Qiuping
Chen, Wenxue
Chen, Weijun
Yun, Yong-Huan
Chen, Haiming
author_sort Zuo, Si-Min
collection PubMed
description Dendrobium officinale, a precious herbal medicine, has been used for a long time in Chinese history. The metabolites of D. officinale, regarded as its effective components to fight diseases, are significantly affected by cultivation substrates. In this study, ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS) was conducted to analyze D. officinale stems cultured in three different substrates: pine bark (PB), coconut coir (CC), and a pine bark: coconut coir 1:1 mix (PC). A total of 529 metabolites were identified. Multivariate statistical analysis methods were employed to analyze the difference in the content of metabolites extracted from different groups. By the criteria of variable importance in projection (VIP) value ≥1 and absolute log(2) (fold change) ≥1, there were a total of 68, 51, and 57 metabolites, with significant differences in content across groups being filtrated out between PB and PC, PB and CC, and PC and CC, respectively. The comparisons among the three groups revealed that flavonoids were the metabolites that fluctuated most. The results suggested the D. officinale stems from the PB group possessed a higher flavonoid content. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that the significantly regulated metabolites were mainly connected with flavonoid biosynthesis. A comprehensive profile of the metabolic differentiation of D. officinale planted in different substrates was provided, which supports the selection of an optimum cultivation substrate for a higher biomass yield of D. officinale.
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spelling pubmed-74654622020-09-04 Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates Zuo, Si-Min Yu, Hai-Dong Zhang, Weimin Zhong, Qiuping Chen, Wenxue Chen, Weijun Yun, Yong-Huan Chen, Haiming Metabolites Article Dendrobium officinale, a precious herbal medicine, has been used for a long time in Chinese history. The metabolites of D. officinale, regarded as its effective components to fight diseases, are significantly affected by cultivation substrates. In this study, ultra-performance liquid chromatography mass spectrometry (UPLC-MS/MS) was conducted to analyze D. officinale stems cultured in three different substrates: pine bark (PB), coconut coir (CC), and a pine bark: coconut coir 1:1 mix (PC). A total of 529 metabolites were identified. Multivariate statistical analysis methods were employed to analyze the difference in the content of metabolites extracted from different groups. By the criteria of variable importance in projection (VIP) value ≥1 and absolute log(2) (fold change) ≥1, there were a total of 68, 51, and 57 metabolites, with significant differences in content across groups being filtrated out between PB and PC, PB and CC, and PC and CC, respectively. The comparisons among the three groups revealed that flavonoids were the metabolites that fluctuated most. The results suggested the D. officinale stems from the PB group possessed a higher flavonoid content. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that the significantly regulated metabolites were mainly connected with flavonoid biosynthesis. A comprehensive profile of the metabolic differentiation of D. officinale planted in different substrates was provided, which supports the selection of an optimum cultivation substrate for a higher biomass yield of D. officinale. MDPI 2020-08-10 /pmc/articles/PMC7465462/ /pubmed/32785071 http://dx.doi.org/10.3390/metabo10080325 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zuo, Si-Min
Yu, Hai-Dong
Zhang, Weimin
Zhong, Qiuping
Chen, Wenxue
Chen, Weijun
Yun, Yong-Huan
Chen, Haiming
Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title_full Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title_fullStr Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title_full_unstemmed Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title_short Comparative Metabolomic Analysis of Dendrobium officinale under Different Cultivation Substrates
title_sort comparative metabolomic analysis of dendrobium officinale under different cultivation substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465462/
https://www.ncbi.nlm.nih.gov/pubmed/32785071
http://dx.doi.org/10.3390/metabo10080325
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