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Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation

Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (P...

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Autores principales: Vishwakarma, Sushma, Gupta, Rishikesh Kumar, Jakati, Saumya, Tyagi, Mudit, Pappuru, Rajeev Reddy, Reddig, Keith, Hendricks, Gregory, Volkert, Michael R., Khanna, Hemant, Chhablani, Jay, Kaur, Inderjeet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465764/
https://www.ncbi.nlm.nih.gov/pubmed/32717933
http://dx.doi.org/10.3390/antiox9080654
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author Vishwakarma, Sushma
Gupta, Rishikesh Kumar
Jakati, Saumya
Tyagi, Mudit
Pappuru, Rajeev Reddy
Reddig, Keith
Hendricks, Gregory
Volkert, Michael R.
Khanna, Hemant
Chhablani, Jay
Kaur, Inderjeet
author_facet Vishwakarma, Sushma
Gupta, Rishikesh Kumar
Jakati, Saumya
Tyagi, Mudit
Pappuru, Rajeev Reddy
Reddig, Keith
Hendricks, Gregory
Volkert, Michael R.
Khanna, Hemant
Chhablani, Jay
Kaur, Inderjeet
author_sort Vishwakarma, Sushma
collection PubMed
description Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-α (HIF1-α), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation.
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spelling pubmed-74657642020-09-04 Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation Vishwakarma, Sushma Gupta, Rishikesh Kumar Jakati, Saumya Tyagi, Mudit Pappuru, Rajeev Reddy Reddig, Keith Hendricks, Gregory Volkert, Michael R. Khanna, Hemant Chhablani, Jay Kaur, Inderjeet Antioxidants (Basel) Article Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-α (HIF1-α), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation. MDPI 2020-07-23 /pmc/articles/PMC7465764/ /pubmed/32717933 http://dx.doi.org/10.3390/antiox9080654 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vishwakarma, Sushma
Gupta, Rishikesh Kumar
Jakati, Saumya
Tyagi, Mudit
Pappuru, Rajeev Reddy
Reddig, Keith
Hendricks, Gregory
Volkert, Michael R.
Khanna, Hemant
Chhablani, Jay
Kaur, Inderjeet
Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title_full Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title_fullStr Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title_full_unstemmed Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title_short Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
title_sort molecular assessment of epiretinal membrane: activated microglia, oxidative stress and inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465764/
https://www.ncbi.nlm.nih.gov/pubmed/32717933
http://dx.doi.org/10.3390/antiox9080654
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