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Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation
Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (P...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465764/ https://www.ncbi.nlm.nih.gov/pubmed/32717933 http://dx.doi.org/10.3390/antiox9080654 |
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author | Vishwakarma, Sushma Gupta, Rishikesh Kumar Jakati, Saumya Tyagi, Mudit Pappuru, Rajeev Reddy Reddig, Keith Hendricks, Gregory Volkert, Michael R. Khanna, Hemant Chhablani, Jay Kaur, Inderjeet |
author_facet | Vishwakarma, Sushma Gupta, Rishikesh Kumar Jakati, Saumya Tyagi, Mudit Pappuru, Rajeev Reddy Reddig, Keith Hendricks, Gregory Volkert, Michael R. Khanna, Hemant Chhablani, Jay Kaur, Inderjeet |
author_sort | Vishwakarma, Sushma |
collection | PubMed |
description | Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-α (HIF1-α), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation. |
format | Online Article Text |
id | pubmed-7465764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74657642020-09-04 Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation Vishwakarma, Sushma Gupta, Rishikesh Kumar Jakati, Saumya Tyagi, Mudit Pappuru, Rajeev Reddy Reddig, Keith Hendricks, Gregory Volkert, Michael R. Khanna, Hemant Chhablani, Jay Kaur, Inderjeet Antioxidants (Basel) Article Fibrocellular membrane or epiretinal membrane (ERM) forms on the surface of the inner limiting membrane (ILM) in the inner retina and alters the structure and function of the retina. ERM formation is frequently observed in ocular inflammatory conditions, such as proliferative diabetic retinopathy (PDR) and retinal detachment (RD). Although peeling of the ERM is used as a surgical intervention, it can inadvertently distort the retina. Our goal is to design alternative strategies to tackle ERMs. As a first step, we sought to determine the composition of the ERMs by identifying the constituent cell-types and gene expression signature in patient samples. Using ultrastructural microscopy and immunofluorescence analyses, we found activated microglia, astrocytes, and Müller glia in the ERMs from PDR and RD patients. Moreover, oxidative stress and inflammation associated gene expression was significantly higher in the RD and PDR membranes as compared to the macular hole samples, which are not associated with inflammation. We specifically detected differential expression of hypoxia inducible factor 1-α (HIF1-α), proinflammatory cytokines, and Notch, Wnt, and ERK signaling pathway-associated genes in the RD and PDR samples. Taken together, our results provide new information to potentially develop methods to tackle ERM formation. MDPI 2020-07-23 /pmc/articles/PMC7465764/ /pubmed/32717933 http://dx.doi.org/10.3390/antiox9080654 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vishwakarma, Sushma Gupta, Rishikesh Kumar Jakati, Saumya Tyagi, Mudit Pappuru, Rajeev Reddy Reddig, Keith Hendricks, Gregory Volkert, Michael R. Khanna, Hemant Chhablani, Jay Kaur, Inderjeet Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title | Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title_full | Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title_fullStr | Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title_full_unstemmed | Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title_short | Molecular Assessment of Epiretinal Membrane: Activated Microglia, Oxidative Stress and Inflammation |
title_sort | molecular assessment of epiretinal membrane: activated microglia, oxidative stress and inflammation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465764/ https://www.ncbi.nlm.nih.gov/pubmed/32717933 http://dx.doi.org/10.3390/antiox9080654 |
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