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The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva
Neurodegenerative diseases are generally characterized by the presence of neurotoxic amyloid aggregates underlying progressive neuronal death. Since ancient times, natural compounds have been used as curative agents for human health. Amyloid research is constantly looking for safe natural molecules...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465926/ https://www.ncbi.nlm.nih.gov/pubmed/32784616 http://dx.doi.org/10.3390/ph13080185 |
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author | Vasarri, Marzia Ramazzotti, Matteo Tiribilli, Bruno Barletta, Emanuela Pretti, Carlo Mulinacci, Nadia Degl’Innocenti, Donatella |
author_facet | Vasarri, Marzia Ramazzotti, Matteo Tiribilli, Bruno Barletta, Emanuela Pretti, Carlo Mulinacci, Nadia Degl’Innocenti, Donatella |
author_sort | Vasarri, Marzia |
collection | PubMed |
description | Neurodegenerative diseases are generally characterized by the presence of neurotoxic amyloid aggregates underlying progressive neuronal death. Since ancient times, natural compounds have been used as curative agents for human health. Amyloid research is constantly looking for safe natural molecules capable of blocking toxic amyloid aggregates’ formation. From the marine environment, seaweeds are recognized as rich reservoirs of molecules with multiple bioactivities, including the anti-amyloidogenic activity. Here, hydroalcoholic extracts of two seasonal samples of the Mediterranean red seaweed Halophytis incurva (HIEs) were characterized by the HPLC-DAD-MS analysis. The H. incurva anti-amyloidogenic role was explored by incubating both HIEs with hen egg white lysozyme (HEWL), a well-known protein model widely used in amyloid aggregation experiments. The aggregation kinetics and morphological analysis of amyloid aggregates were performed by ThT and AFM analysis, respectively, while their cytotoxicity on SH-SY5Y human neuroblastoma cells was examined by MTT assay. HIEs showed a different efficacy, probably dependent on their metabolic composition, both in inhibiting amyloid fibrillation and in obtaining short and less toxic pre-fibrillary aggregates. Overall, this work sheds light, for the first time, on a Mediterranean red seaweed as a promising renewable resource of bioactive compounds, potentially useful in preventing the formation of toxic amyloid aggregates. |
format | Online Article Text |
id | pubmed-7465926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74659262020-09-04 The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva Vasarri, Marzia Ramazzotti, Matteo Tiribilli, Bruno Barletta, Emanuela Pretti, Carlo Mulinacci, Nadia Degl’Innocenti, Donatella Pharmaceuticals (Basel) Article Neurodegenerative diseases are generally characterized by the presence of neurotoxic amyloid aggregates underlying progressive neuronal death. Since ancient times, natural compounds have been used as curative agents for human health. Amyloid research is constantly looking for safe natural molecules capable of blocking toxic amyloid aggregates’ formation. From the marine environment, seaweeds are recognized as rich reservoirs of molecules with multiple bioactivities, including the anti-amyloidogenic activity. Here, hydroalcoholic extracts of two seasonal samples of the Mediterranean red seaweed Halophytis incurva (HIEs) were characterized by the HPLC-DAD-MS analysis. The H. incurva anti-amyloidogenic role was explored by incubating both HIEs with hen egg white lysozyme (HEWL), a well-known protein model widely used in amyloid aggregation experiments. The aggregation kinetics and morphological analysis of amyloid aggregates were performed by ThT and AFM analysis, respectively, while their cytotoxicity on SH-SY5Y human neuroblastoma cells was examined by MTT assay. HIEs showed a different efficacy, probably dependent on their metabolic composition, both in inhibiting amyloid fibrillation and in obtaining short and less toxic pre-fibrillary aggregates. Overall, this work sheds light, for the first time, on a Mediterranean red seaweed as a promising renewable resource of bioactive compounds, potentially useful in preventing the formation of toxic amyloid aggregates. MDPI 2020-08-07 /pmc/articles/PMC7465926/ /pubmed/32784616 http://dx.doi.org/10.3390/ph13080185 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vasarri, Marzia Ramazzotti, Matteo Tiribilli, Bruno Barletta, Emanuela Pretti, Carlo Mulinacci, Nadia Degl’Innocenti, Donatella The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title | The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title_full | The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title_fullStr | The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title_full_unstemmed | The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title_short | The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva |
title_sort | in vitro anti-amyloidogenic activity of the mediterranean red seaweed halopithys incurva |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465926/ https://www.ncbi.nlm.nih.gov/pubmed/32784616 http://dx.doi.org/10.3390/ph13080185 |
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