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Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress
Some lichens provide the resources of common traditional medicines and show anticancer effects. However, the anticancer effect of Usnproliea barbata (U. barbata) is rarely investigated, especially for oral cancer cells. The aim of this study was to investigate the cell killing function of methanol e...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465944/ https://www.ncbi.nlm.nih.gov/pubmed/32756347 http://dx.doi.org/10.3390/antiox9080694 |
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author | Tang, Jen-Yang Wu, Kuang-Han Wang, Yen-Yun Farooqi, Ammad Ahmad Huang, Hurng-Wern Yuan, Shyng-Shiou F. Jian, Ru-In Tsao, Li-Yi Chen, Po-An Chang, Fang-Rong Cheng, Yuan-Bin Hu, Hao-Chun Chang, Hsueh-Wei |
author_facet | Tang, Jen-Yang Wu, Kuang-Han Wang, Yen-Yun Farooqi, Ammad Ahmad Huang, Hurng-Wern Yuan, Shyng-Shiou F. Jian, Ru-In Tsao, Li-Yi Chen, Po-An Chang, Fang-Rong Cheng, Yuan-Bin Hu, Hao-Chun Chang, Hsueh-Wei |
author_sort | Tang, Jen-Yang |
collection | PubMed |
description | Some lichens provide the resources of common traditional medicines and show anticancer effects. However, the anticancer effect of Usnproliea barbata (U. barbata) is rarely investigated, especially for oral cancer cells. The aim of this study was to investigate the cell killing function of methanol extracts of U. barbata (MEUB) against oral cancer cells. MEUB shows preferential killing against a number of oral cancer cell lines (Ca9-22, OECM-1, CAL 27, HSC3, and SCC9) but rarely affects normal oral cell lines (HGF-1). Ca9-22 and OECM-1 cells display the highest sensitivity to MEUB and were chosen for concentration effect and time course experiments to address its cytotoxic mechanisms. MEUB induces apoptosis of oral cancer cells in terms of the findings from flow cytometric assays and Western blotting, such as subG1 accumulation, annexin V detection, and pancaspase activation as well as poly (ADP-ribose) polymerase (PARP) cleavage. MEUB induces oxidative stress and DNA damage of oral cancer cells following flow cytometric assays, such as reactive oxygen species (ROS)/mitochondrial superoxide (MitoSOX) production, mitochondrial membrane potential (MMP) depletion as well as overexpression of γH2AX and 8-oxo-2′deoxyguanosine (8-oxodG). All MEUB-induced changes in oral cancer cells were triggered by oxidative stress which was validated by pretreatment with antioxidant N-acetylcysteine (NAC). In conclusion, MEUB causes preferential killing of oral cancer cells and is associated with oxidative stress, apoptosis, and DNA damage. |
format | Online Article Text |
id | pubmed-7465944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74659442020-09-04 Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress Tang, Jen-Yang Wu, Kuang-Han Wang, Yen-Yun Farooqi, Ammad Ahmad Huang, Hurng-Wern Yuan, Shyng-Shiou F. Jian, Ru-In Tsao, Li-Yi Chen, Po-An Chang, Fang-Rong Cheng, Yuan-Bin Hu, Hao-Chun Chang, Hsueh-Wei Antioxidants (Basel) Article Some lichens provide the resources of common traditional medicines and show anticancer effects. However, the anticancer effect of Usnproliea barbata (U. barbata) is rarely investigated, especially for oral cancer cells. The aim of this study was to investigate the cell killing function of methanol extracts of U. barbata (MEUB) against oral cancer cells. MEUB shows preferential killing against a number of oral cancer cell lines (Ca9-22, OECM-1, CAL 27, HSC3, and SCC9) but rarely affects normal oral cell lines (HGF-1). Ca9-22 and OECM-1 cells display the highest sensitivity to MEUB and were chosen for concentration effect and time course experiments to address its cytotoxic mechanisms. MEUB induces apoptosis of oral cancer cells in terms of the findings from flow cytometric assays and Western blotting, such as subG1 accumulation, annexin V detection, and pancaspase activation as well as poly (ADP-ribose) polymerase (PARP) cleavage. MEUB induces oxidative stress and DNA damage of oral cancer cells following flow cytometric assays, such as reactive oxygen species (ROS)/mitochondrial superoxide (MitoSOX) production, mitochondrial membrane potential (MMP) depletion as well as overexpression of γH2AX and 8-oxo-2′deoxyguanosine (8-oxodG). All MEUB-induced changes in oral cancer cells were triggered by oxidative stress which was validated by pretreatment with antioxidant N-acetylcysteine (NAC). In conclusion, MEUB causes preferential killing of oral cancer cells and is associated with oxidative stress, apoptosis, and DNA damage. MDPI 2020-08-03 /pmc/articles/PMC7465944/ /pubmed/32756347 http://dx.doi.org/10.3390/antiox9080694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Jen-Yang Wu, Kuang-Han Wang, Yen-Yun Farooqi, Ammad Ahmad Huang, Hurng-Wern Yuan, Shyng-Shiou F. Jian, Ru-In Tsao, Li-Yi Chen, Po-An Chang, Fang-Rong Cheng, Yuan-Bin Hu, Hao-Chun Chang, Hsueh-Wei Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title | Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title_full | Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title_fullStr | Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title_full_unstemmed | Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title_short | Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress |
title_sort | methanol extract of usnea barbata induces cell killing, apoptosis, and dna damage against oral cancer cells through oxidative stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465944/ https://www.ncbi.nlm.nih.gov/pubmed/32756347 http://dx.doi.org/10.3390/antiox9080694 |
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